47 results about "Aromatic hydrocarbon receptor" patented technology

Uses of ethyl 5,11-dihydroindolo[3,2-b]carbazole-6-carboxylate as a fluorescent ligand probe, preferably in a binding assay for quantitative analysis in combination with a recombinant aryl hydrocarbon receptor (AhR) protein. A method for detection or quantitative analysis of suspected aryl hydrocarbon receptor (AhR) ligands in a sample, the method comprising the steps of: (a) providing a sample possibly containing at least one known or unknown AhR ligand; (b) mixing said sample with a composition comprising a recombinant AhR protein bound to ethyl 5,11-dihydroindolo[3,2-b]carbazole-6-carboxylate; and (c) determining the presence or the total amount of said at least one known or unknown AhR ligand in the sample by fluorescence spectroscopy. Use e.g. for screening of unknown compounds to determine their binding ability to AhR; for analyzing unknown chemical mixtures to assess the presence of ligands able to bind to AhR; for screening of Polycyclic Aromatic Hydrocarbon (PAHs) from water, food or sediment; for screening of PAHs or dioxin-like compounds in water, food or sediment; for testing for pollutant removal in water and waste water treatment plants; for testing for water reuse; for testing the presence of dioxin-like compounds in incinerator plants; or for testing of bodily fluids of humans or animals exposed to PAHs or dioxin-like compounds.

The present invention provides a new application of aryl hydrocarbon receptor (AhR) in predicting the effect of immunotherapy, specifically, the present invention provides the application of AhR as a target in the development, screening and / or preparation of drugs for treating tumors , also provides the application of the reagents for inhibiting, silencing and / or knocking out AhR in the preparation of drugs for treating tumors, and also provides the use of reagents for detecting the level of AhR in the preparation for predicting the effect of immune checkpoint inhibitors in treating tumors application in agents.

The invention discloses a compound 5-(4-hydroxyl-3-methoxyl benzylidene)-2-(2-nitryl phenylimino) thiazolidone, HMNT for short, and an application thereof in an aromatic hydrocarbon receptor stimulant, wherein the chemical structure is as shown in a formula (I). Experiments verify that the compound disclosed by the invention effectively causes up-regulation of genetic expression and protein expression of a metabolic enzyme CYP1A1 of a related allogenic material of a downstream target gene of an aromatic hydrocarbon receptor in a mouse hepatoma carcinoma cell at the concentration of 10 mu M and prompts that the compound is the aromatic hydrocarbon receptor stimulant, is expected to become a potential drug by taking the aromatic hydrocarbon receptor as a tumor treating target, and is wide in clinical application prospect.