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Detection method of tetracycline

A detection method, the technology of tetracycline, applied in the detection field, can solve the problems of expensive equipment, time-consuming and complicated, and unfriendly organic solvents, and achieve the effect of high detection sensitivity and fast detection method

Active Publication Date: 2021-06-25
TCL CORPORATION
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In view of the above-mentioned deficiencies in the prior art, the object of the present invention is to provide a detection method for tetracycline, which aims to solve the problem that the existing method requires expensive instruments and equipment, time-consuming and complicated pretreatment procedures, and uses some organic compounds in the detection process. The problem of solvents being unfriendly to the environment

Method used

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  • Detection method of tetracycline
  • Detection method of tetracycline

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Experimental program
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Embodiment 1

[0034] Example 1: Next, MOS 2 The nanoflakes are described in a detailed description of the above synthesis.

[0035] Preparation of a piece of MOS in 6 layers 2 Nanoflane, dissolved in Tris-HCl buffer solution;

[0036] A surface modified CDSE quantum dot is modified;

[0037] Preparation of a tetracycline adapter single-strand DNA: -HOOC-CGT ACG ATG CAG-COOH;

[0038] -HOOC-CGT ACG ATG CAG-COOH is dissolved in pH = 6.0, Tris-HCl buffer solution, and stirred for 2 hours, resulting in quantum dots - adapter solution;

[0039] Wrap the laminate MOS 2 The solution is added to the quantum dot - adapter solution, quench the fluorescence of the quantum dots - adapter;

[0040] Further, a tetracycline solution was added to the above solution, and the fluorescence intensity of the system was measured after 10 minutes of incubation for 30 minutes, and the system was fluorescence.

Embodiment 2

[0041] Example 2: The following is VTE 2 The nanoflakes are described in a detailed description of the above synthesis.

[0042] Preparation of a piece of VTE for 10 layers 2 Nanoflane, dissolved in phosphate buffered solution;

[0043] A surface modified INASP quantum dot is modified;

[0044] Preparation of a tetracycline adapter single-stranded RNA: -HOOC-CGT ACG CUA UAG CGU CAG-COOH;

[0045] Plus -HOOC-CGT ACG ATG CAG-COOH and the surface modified an amino-based INASP quantum dot is dissolved in pH = 7.0, phosphate buffered solution, stirred for 20 hours;

[0046] Wrap the sheet-like VTE 2 The solution is added to the quantum dot - adapter solution, quench the fluorescence of the quantum dots - adapter;

[0047] Then, a tetracycline solution was added to the above solution, and the fluorescence intensity of the system was measured after incubation at room temperature, and the detection was fluorescence.

[0048] In summary, the method of detecting a tetracycline provided by t...

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Abstract

The invention discloses a detection method of tetracycline. The detection method of tetracycline comprises the steps that a quantum dot-aptamer solution is provided, quantum dots in a quantum dot-aptamer are combined with an aptamer, and the aptamer is single-stranded DNA or single-stranded RNA; the quantum dot-aptamer is combined with a layered transition metal compound; and a to-be-detected solution is added into the solution, and it is detected that the to-be-detected solution contains tetracycline if fluorescence appears in the solution. According to the method provided by the invention, the tetracycline is detected by taking a transition metal compound with a layered structure as a biological switch and a quantum dot as a fluorescent marker. The detection method is rapid and high in detection sensitivity.

Description

Technical field [0001] The present invention relates to the field of detection, and more particularly to a method of detecting a tetracycline. Background technique [0002] TetracyCline (TET) is a common antibiotic due to its advantages such as good antibacterial, low price, low side effects, and is widely used in human diseases and drug additives. However, due to the excessive abuse of Tet and Tet is highly soluble in water, it has caused very serious pollution on the surface water and groundwater. The residual tetracycline can be transmitted to the human body through the food chain. With the accumulation of tetracycline in the human body, a series of side effects, such as bacterial resistance, causing the liver damage, affecting the development of the bones, and weakening the function of the human immune system, etc. . [0003] In recent years, a two-dimensional (2D) transition metal nano-quarantine point material having a layer structure, including transition metal disulfide (...

Claims

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Application Information

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IPC IPC(8): G01N21/64
CPCG01N21/6428G01N2021/6432
Inventor 邓承雨芦子哲
Owner TCL CORPORATION