Production process of high-binding-force elisa plate

A production process and microplate technology, which is applied in the field of biological instruments, can solve the problems of increasing the amount of protein and weak binding ability between microplate and protein, and achieve the effect of smooth plate surface and increased rigidity

Pending Publication Date: 2021-06-25
苏州赛普生物科技股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] In order to solve the problem in the prior art that the binding ability between the enzyme label plate and protein is weak and the

Method used

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  • Production process of high-binding-force elisa plate
  • Production process of high-binding-force elisa plate
  • Production process of high-binding-force elisa plate

Examples

Experimental program
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Embodiment 1

[0064] The present embodiment provides a kind of production technique of high-binding force microtiter plate, comprises the following steps:

[0065] S1: The polystyrene beads and steam are continuously mixed in a pre-foaming machine under the stirring of the stirring tank to complete the foaming reaction. The pre-expanding machine is kept open at atmospheric pressure, and the polystyrene beads that have been expanded are overflowed from the top of the pre-expanding machine. After the polystyrene beads are foamed, aging treatment is carried out so that air is mixed into the cells to obtain aged pre-expanded beads. Put the cured pre-expanded beads into the molding equipment for molding treatment to obtain a 12×8 96-well microplate.

[0066] S2: Place the microplate prepared in step S1 in the plasma surface treatment equipment, open the reaction gas inlet valve 2 and the exhaust valve 5, and pass compressed air into the plasma surface treatment equipment, and the compressed air...

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Abstract

The invention relates to a production process of a high-binding-force elisa plate. The process comprises the following steps: S1, foaming, curing and forming expandable polystyrene to prepare a foamed polystyrene elisa plate. S2, placing the elisa plate in plasma surface treatment equipment, introducing first gas, and cleaning the surface of the elisa plate. and S3, supplementing second gas into the plasma surface treatment equipment, ionizing positive and negative charges from the second gas in the plasma surface treatment equipment, and performing surface hydrophilic modification treatment on the elisa plate to obtain the high-binding-force elisa plate. According to the invention, amino groups with positive charges are generated on the surface of the elisa plate after surface modification treatment, the hydrophobic bonds on the surface of the elisa plate are replaced by hydrophilic bonds, the surface of the elisa plate can be combined with proteins with negative charges through ionic bonds under proper buffer solution and pH value, and the elisa plate subjected to surface hydrophilic modification treatment has high sensitivity to protein in the experiment process, so that the concentration and dosage of coated protein can be reduced.

Description

technical field [0001] The invention belongs to the technical field of biological instruments, and in particular relates to a production process of a high-binding enzyme label plate. Background technique [0002] The microtiter plate is mainly used in the laboratory to carry various necessary experimental supplies, and plays a key role in conditions such as antigens, antibodies, labeled antibodies, and buffers involved in immunological reactions. And the solid-phase polystyrene surface as the carrier of the microtiter plate also plays a very important role in the adsorption of antigens, antibodies or their complexes. Antigens, antibodies, and biomolecules are adsorbed to the surface of the microtiter plate, including passive adsorption, covalent binding by introducing other active groups, and binding by hydrophilic bonds. [0003] During the experimental determination of the microplate plate, the tested specimen reacts with the antigen or antibody on the surface of the micr...

Claims

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Application Information

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IPC IPC(8): G01N33/543
CPCG01N33/54393
Inventor 张胜有叶竹青季开文
Owner 苏州赛普生物科技股份有限公司
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