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Method for extracting licoflavone by using R19

A technology of licorice flavonoids and R19, which is applied in the field of medicine and chemical industry, can solve the problems of low glycyrrhizic acid content, environmental pollution, large output, etc., and achieve the effect of simple process, clear process and high yield

Inactive Publication Date: 2021-06-29
JIANGSU TIANSHENG PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] R19 is the glycyrrhizic acid powder with a glycyrrhizic acid content of about 26% after extraction, ammoniation, decolorization, and crystallization. A lot of R19, due to the large yield, low glycyrrhizic acid content and enrichment of other impurities, if the glycyrrhizic acid is re-extracted, the cost will be too high, but if it is discarded, it will not only be wasteful, but also pollute the environment. Therefore, there is an urgent need for a suitable R19 Approach
[0003] By consulting relevant literature, R19 was analyzed by liquid phase, and it was found that R19 contained more flavonoids. At present, the research on flavonoids in licorice and flavonoids in licorice residue has been relatively mature, but the treatment of R19 The application is relatively rare, because a certain flavonoid in licorice has phytoestrogens properties, which can inhibit the growth of some cancer cells. At the same time, flavonoids in licorice generally have the ability to scavenge free radicals and have antiviral and antibacterial effects

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] In this embodiment, the specific operation steps are as follows:

[0026] S1: Preparation of Solution: R19 1 kg is taken as a raw material, and 2.5 l50% ethanol is added thereto, stirred at 40 ° C, stirred, dissolved, and place the chamber to prepare a solution.

[0027] S2: Macroporous resin treatment: Soak the AB-8 macroporous resin for 24 h, packed column, flush the column with pure water, to the free soy saving, the large pore resin is 15 kg of the sample weight, the resin column is high The ratio is 1:10.

[0028] S3: Sample: Add the sample solution to the resin column, and turn on the plunger, the control flow rate is 1 column volume per hour. After the sample solution is added, the plunger is closed.

[0029] S4: Elution: Use water as the eluent to elution, the amount is 3 column volume, and then 20% ethanol, 40% ethanol, 50% ethanol, 70% ethanol and 95% ethanol, using TLC to elution Monitoring, 20% ethanol and 40% ethanol were used as 2.5 column volume, 50% ethanol ...

Embodiment 2

[0033] In this embodiment, the specific operation steps are as follows:

[0034] S1: Substituted Solution: Take R19 2 kg, 6L50% ethanol, stirred at 40 ° C, dissolved, dissolved, and place the chamber to give a solution.

[0035] S2: Macole resin treatment: XAD-1 macroporous resin is soaked in 95% ethanol 24h, column, rinse the column with pure water, to no alcohol, large pore resin is 35kg of the sample weight, the resin column is high The ratio is 1:13.

[0036] S3: Sample: Add the sample solution to the resin column, and turn on the plunger, the control flow rate is 1 column volume per hour. After the sample solution is added, the plunger is closed. The use of water as the eluent begins to elution, the amount is 3 column volume, and then 20% ethanol, 40% ethanol, 50% ethanol, 70% ethanol and 95% ethanol are seated, and each eluate liquid is monitored by TLC, 20 % Ethanol and 40% ethanol are used as 3 column volume, 50% ethanol and 70% ethanol amount are 3 column volume, and 95% ...

Embodiment 3

[0039] In this embodiment, the specific operation steps are as follows:

[0040] S1: Sample Solution Preparation: Take R19 2 kg, adding 6.5 L 50% ethanol, stirred at 50 ° C for water dissolving, after dissolving, the chamber is placed on a solution.

[0041] S2: Macole Resin Treatment: Soaked the model of 95% ethanol for 24 h with 95% ethanol, pure water, and the amount of the large pore resin is 37kg, the resin post High ratio is 1: 15.

[0042]S3: Sample: Add the sample solution to the resin column, and turn on the plunger, the control flow rate is 1 column volume per hour. After the sample solution is added, the plunger is closed. The water was eluted as the eluent, and the amount was 4 column volumes, and then 20% ethanol, 40% ethanol, 50% ethanol, 70% ethanol and 95% ethanol were subjected to monitoring of each eluent using TLC. % Ethanol and 40% ethanol were used as 3 column volume, 50% ethanol and 70% ethanol amount of 4 column volume, 95% ethanol amount of 2 column volume,...

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Abstract

The invention discloses a method for extracting licoflavone by using R19. The preparation method comprises the following steps of: taking R19 as a raw material, dissolving the R19 in water to obtain a sample loading solution, then treating macroporous resin, soaking the macroporous resin with an ethanol-water solution, packing the macroporous resin, and washing the macroporous resin with pure water until no alcohol smell exists; eluting by using water as an eluent, and detecting through TLC to obtain a flavonoid compound; and then purifying the flavonoid compound by using a polyamide column to finally obtain the high-content flavonoid compound. The method for extracting licoflavone by using R19 is clear in process, simple in process, low in cost and high in yield, and provides a more efficient way for preparing licoflavone.

Description

Technical field [0001] The present invention relates to a method of extracting a total flavonoid, in particular to a method of extracting total flavonoids in R19 (ammonium glycyrrhizine parents), which belongs to the technical field of medical chemical technology. Background technique [0002] R19 is a glycyrrhizic acid content of about 26%, and the diaphragm having a crystalline, a glycyrrhizic acid, glycyrrhizine, glycyrrhid, glycyrrhizen, and ammonium glycyrrhiza, a large amount of glycyrrhizic acid. Maximum R19, due to high yield, low glycyrrhizic acid content and other impurities, if the cost of glycyrrhizal acid is too high, but if abandoned, the environment is not only very wasteful, and will pollute the environment, there is an urgent need for R19 Approach. [0003] By reviewing the relevant literature, the ingredient analysis of R19 is performed by liquid phase, and it is found that there is more flavonoid compounds in R19. At present, the study of flavonoids in licorice...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/484
CPCA61K36/484A61K2236/55
Inventor 季浩胡亚京阚建伟刘佳窦长清孔繁博
Owner JIANGSU TIANSHENG PHARMA
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