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Liquid drop type PCR chip and liquid drop type PCR detection system

A detection system and droplet technology, applied in the field of microfluidic droplet digital polymerase chain reaction, can solve problems such as easy formation of bubbles

Pending Publication Date: 2021-07-06
中山百慧生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] This application provides a droplet-type PCR chip and a droplet-type PCR detection system to solve the technical problem that the micro-droplet chip is easy to form bubbles in the PCR reaction process

Method used

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  • Liquid drop type PCR chip and liquid drop type PCR detection system
  • Liquid drop type PCR chip and liquid drop type PCR detection system
  • Liquid drop type PCR chip and liquid drop type PCR detection system

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Embodiment Construction

[0021] The technical solutions in the embodiments of the present application will be clearly and completely described below with reference to the drawings in the embodiments of the present application. Obviously, the described embodiments are only a part of the embodiments of the present application, but not all of the embodiments. Based on the embodiments in the present application, all other embodiments obtained by those of ordinary skill in the art without creative efforts shall fall within the protection scope of the present application.

[0022] see Figure 1 to Figure 2 , figure 1 It is a schematic diagram of the overall structure of an embodiment of the droplet PCR chip of the present application; figure 2 It is a schematic structural diagram of a side view of an embodiment of the droplet type PCR chip of the present application.

[0023] An embodiment of the present application provides a droplet type PCR chip 10, such as figure 1 and figure 2 As shown, at least ...

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Abstract

The invention discloses a liquid drop type PCR (Polymerase Chain Reaction) chip and a liquid drop type PCR detection system. The liquid drop type PCR chip comprises at least one unit, wherein each unit comprises an oil phase liquid storage tank, a PCR reagent liquid storage tank, a liquid drop tiling cavity and an outlet liquid storage tank; the PCR reagent liquid storage tank and the oil phase liquid storage tank are converged through a fluid flow path and converge to a droplet generation channel; one end of the liquid drop tiling cavity communicates with the liquid drop generation channel so as to receive the generated liquid drops, and the liquid drop tiling cavity provides a PCR amplification reaction place for the liquid drops; the outlet liquid storage tank is connected to the other end of the liquid drop tiling cavity so as to discharge air extruded by the liquid drops in the liquid drop tiling cavity; and each liquid storage tank is also used for receiving mineral oil so as to seal the liquid drop tiling cavity. Therefore, liquid drops are prevented from being squeezed out of the liquid drop tiling cavity due to leakage of bubbles generated by heating in oil phase; and in addition, the vapor pressure generated by heating in the oil phase is retained in the liquid drop type PCR chip after sealing, so that the vapor pressure in the liquid drop tiling cavity is improved, and bubbles generated in the liquid drop type PCR chip can be reduced or avoided.

Description

technical field [0001] The present application belongs to the technical field of microfluidic droplet digital polymerase chain reaction (PCR), and in particular relates to a droplet PCR chip and a droplet PCR detection system. Background technique [0002] The microdroplet chip is developed on the basis of the traditional single-phase microfluidic chip technology. Compared with the single-phase microfluidic system, due to its characteristics of water / oil two-phase separation, it has the advantages such as consumption of samples and reagents. Less volume, faster mixing speed, less likely to cause cross-contamination, easy to handle and other advantages. [0003] The droplets of the existing micro-droplet chips are stored in the PCR reaction area after being generated, and the boiling point of the commonly used oil for droplet generation is about 130°C. Therefore, in the conventional PCR reaction, when the heating temperature is 95°C, the There will be bubble formation, and t...

Claims

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Application Information

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IPC IPC(8): C12M1/00C12Q1/686
CPCB01L3/50851C12Q1/686C12Q2563/159
Inventor 于浩洋李彦虎吴慧君
Owner 中山百慧生物科技有限公司
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