Aflatoxin detecting and sorting device and aflatoxin detecting and sorting method

Abstract

The invention discloses an aflatoxin detecting and sorting device, which comprises a material sliding way; ultraviolet light sources are arranged on the two sides of the tail end of the material sliding way; light splitting devices are arranged on the two sides of the ultraviolet light sources; a visible light gray level line-scan digital camera set is arranged beside the light splitting devices; the visible light gray level line-scan digital camera set is composed of two visible light gray level line-scan digital cameras with optical axes perpendicular to each other; a central wavelength lambda2 optical filter is arranged in front of one visible light gray level line-scan digital camera; and a central wavelength lambda1 optical filter is arranged in front of the other visible light gray level line-scan digital camera. Compared with the prior art, the aflatoxin detecting and sorting device provided by the invention has the advantages that when the visible light gray level line-scan digital camera set collects fluorescence signals, the fluorescence signals are in the same light path in front of the light splitting device, that is, fluorescence images obtained by two cameras always come from the same position of a material, it is ensured that a calculated normalized difference fluorescence index can accurately reflect the degree that the material is polluted by aflatoxin, and the aflatoxin detection and sorting precision and efficiency can be improved.

Description

technical field [0001] The invention relates to the field of detection and sorting devices, in particular to an aflatoxin detection and sorting device and a detection and sorting method. Background technique [0002] Aflatoxin is a heterocyclic compound produced by Aspergillus flavus and Aspergillus parasiticus. Its toxicity is much higher than that of cyanide, arsenic and organic pesticides, and it has a strong carcinogenic effect. Aflatoxin is easy to contaminate grain and oil and other agricultural products, food and feed, and directly or indirectly enters the human food chain. It is one of the major issues affecting the quality of agricultural products and food safety, and has attracted great attention from all over the world. [0003] The detection of aflatoxin generally adopts chemical methods: thin-layer chromatography (TLC), high-performance liquid chromatography (HPLC) and enzyme-linked immunosorbent assay (ELISA). The number of samples tested was limited, resultin...

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Problems solved by technology

[0007] The TDI CCD image sensor has high sensitivity and signal-to-noise ratio, and can effectively detect weak fluorescent signals on the surface of the material, but the TDI CCD image sensor is expensive, and the line scanning frequency is strictly synchronized with the movement rate of the target when used.

At the same time, the synthetic RGB image analysis accuracy is not high, and the analysis results can only detect the presence or absence of aflatoxin, and cannot establish a direct relationship with the aflatoxin content of the material

[0008] Since the fluorescent signal generated on the surface of the material is very weak, and the spectral range of the visible LED covers the fluorescent spectrum, when the visible LED and the ultraviolet LED are used to illuminate together, it will interfere with the collection of the fluorescent signal by the camera, and even cover up the fluorescent signal; at the same time, for two different For the acquisition of wavelength fluorescence images, two flat mirrors are used on the optical path to reflect the fluorescence signals at the same position on the material. This detection method is related to the spatial position of the material falling, and it cannot be guaranteed that the acquired fluorescence images of two wavelengths are from the material. In the same position on the above, there is an error in the normalized difference fluorescence index calculated in this way, which will affect the detection of whether the material is contaminated by aflatoxin

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