Sperm counting method in egg zona pellucida binding test

Abstract

The invention provides an optimized sperm counting method for an egg zona pellucida binding test. The method is easy and convenient to operate, used reagents are easy to obtain, the test cost can be remarkably reduced, and the technical problem that clinical popularization is limited due to the fact that operation is complex and the requirement for detection personnel is too high is solved.

Description

technical field

[0001] The invention relates to an optimized zona pellucida binding experiment method, more specifically, the invention relates to a sperm counting method in the egg zona pellucida binding test. Background technique

[0002] Infertility is defined as the failure of a couple to achieve a clinical pregnancy after normal and frequent unprotected intercourse for 12 months or more. In developed countries, the incidence of infertility is 5.0-8.0%, while in developing countries it is as high as 5.8-44.2%, of which infertility due to male factors accounts for 40-50%, and about 2% of the sperm parameters of pregnant men Not up to par. At present, the clinical laboratory diagnosis of male infertility is mainly based on the "WHO Human Semen Examination and Processing Laboratory Manual (5th Edition)", using computer-aided sperm analysis (CASA) for routine semen analysis. Combining computer processing technology and advanced image processing technology, through a compre...

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