A specific T cell receptor targeting cytomegalovirus antigen and its application
A cytomegalovirus and cell receptor technology, which is applied in the field of specific T cell receptors targeting cytomegalovirus antigens, can solve the problems of lack of TCR pairing information, complicated screening process, etc., and achieve the effect of simple and easy-to-operate preparation methods.
Active Publication Date: 2022-05-31
SHENZHEN HUADA GENE INST
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Problems solved by technology
However, its complex screening process and lack of TCR pairing information have become obstacles to its wide application
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[0060] In this case, the antigen CMV pp65 antigen NLV epitope polypeptide NLVPMVATV based on HLA-A*0201 type was induced to expand antigen-specific cytotoxic T lymphocytes in vitro, and the positive T cells were sorted by single-cell sequencing and MHC tetramer. According to the TCR pairing information, TCR was screened according to the clone sequence frequency, TCR-T was constructed, and the function of TCR-T was verified in vitro, and the function of TCR-T was evaluated, and finally the specific T cell receptor of this case was determined. Details are as follows:
[0061] 1. Induction and functional detection of CMV pp65 NLV-specific cytotoxic T lymphocytes
[0062] 1.1 Induction of CMV pp65 NLV-specific cytotoxic T lymphocytes
[0063] 50 mL of HLA-A*0201-typed healthy human peripheral blood was collected, and Ficoll density gradient centrifugation was used to obtain peripheral blood mononuclear cells (PBMC). From the isolated PBMCs, CD14 was sorted using anti-CD14 antibo...
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The present application discloses a specific T cell receptor targeting cytomegalovirus antigen and its application. This application targets the specific T cell receptor of cytomegalovirus antigen, which can specifically target and bind cytomegalovirus phosphoprotein pp65. The α chain and β chain of the specific T cell receptor each have three complementarity determining regions. The three complementarity determining regions of the α chain are sequences shown in SEQ ID No.4, 5, and 2 in sequence; the three complementarity determining regions of the β chain are sequences shown in SEQ ID No.6, 7, and 3 in sequence. The specific T cell receptor of the present application can specifically target the epitope polypeptide that binds cytomegalovirus phosphoprotein pp65, and provides a new solution for TCR-T treatment of cytomegalovirus-related diseases; and, The preparation method of the specific T cell receptor of the present application is simple and easy to operate, which lays a foundation for the popularization and application of TCR-T therapy based on the specific T cell receptor.
Description
technical field [0001] The present application relates to the field of cytomegalovirus, in particular to a specific T cell receptor targeting cytomegalovirus antigen and its application. Background technique [0002] Cytomegalovirus (cytomegalovirus, CMV) is a member of the herpes virus family. The symptoms of cytomegalovirus infection vary greatly, from asymptomatic to general malaise and fever. The immunocompromised person and the fetus may have severe symptoms, such as blindness, brain and internal organs damage. The virus can be transmitted through sexual or non-sexual body fluids. Meanwhile, CMV infection is also common in organ transplantation and bone marrow transplantation, and is an important factor causing disease and death after allogeneic hematopoietic stem cell transplantation (allo-HSCT). However, current antiviral drugs, such as acyclovir, valganciclovir, ganciclovir, often cause severe side effects, and with the development of drug-resistant virus strains, ...
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IPC IPC(8): C07K14/725C12N15/12C12N15/867C12N5/10A61K38/17A61K35/17A61P31/22
CPCC07K14/7051C12N15/86C12N5/0636A61K35/17A61P31/22C12N2740/15043C12N2800/107C12N2510/00A61K38/00
Inventor 徐曲苗朱琳楠王飞赵正琦林玉琪李子怡顾颖侯勇
Owner SHENZHEN HUADA GENE INST