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Fruit phytophthora infestans detection method and application thereof

A technology of Phytophthora infestans and detection methods, applied in biochemical equipment and methods, microbial determination/inspection, etc., can solve problems such as inability to distinguish closely related species, and achieve simple and reliable principles, high detection efficiency, and rapid detection methods. Effect

Pending Publication Date: 2021-07-16
SHAANXI NORMAL UNIV +1
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  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0007] The purpose of the present invention is to establish an accurate and fast method for detecting Phytophthora infestans strains of fruits, which can detect multiple pathogenic microorganisms at the same time, especially to solve the problem that related species cannot be distinguished in existing detection methods

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  • Fruit phytophthora infestans detection method and application thereof
  • Fruit phytophthora infestans detection method and application thereof
  • Fruit phytophthora infestans detection method and application thereof

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Embodiment Construction

[0039] In the following, the technical solutions of the present invention will be further described in detail in conjunction with the accompanying drawings, but the present invention is not limited to the following embodiments.

[0040] The test strains used in the present invention are from Nanjing Forestry University, Fujian Academy of Agricultural Sciences and China Agricultural Microorganisms Preservation Center, including Chestnut Phytophthora (Phytophthoracambivora), Cedar Phytophthora (P.lateralis), Phaseoli Phytophthora (P.phaseoli), Phytophthora cloves (P.syringae), Phytophthora lychee (P.litchii), Phytophthora strawberry (P.fragariae, P.nicotianane, P.cactorum), Phytophthora melonis (P.melonis), Phytophthora citrus ( P.hibernalis, P.citricola, P.citrophthora), oak sudden death Phytophthora (P.ramorum), raspberry Phytophthora (P.rubi), camphor Phytophthora (P.cinnamomi) and palm Phytophthora (P.palmivora ).

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Abstract

The invention provides a fruit phytophthora infestans detection method and an application thereof. The fruit phytophthora infestans detection method comprises specific steps of extracting phytophthora strain DNA, and performing target gene Ypt1 amplification; screening general restriction endonuclease for phytophthora strains, and performing enzyme digestion on the target gene Ypt1 to amplify a gene segment; differentiating capillary electrophoretograms of different phytophthora strains; and comparing and analyzing the capillary electrophoretogram of the plant tissue to be detected and the phytophthora strains, and identifying the phytophthora strains infecting the plant tissue to be detected according to the position of a wave crest in the capillary electrophoretogram. The method can be used for simultaneously detecting multiple pathogenic microorganisms, particularly solves the problem that the sibling species cannot be distinguished in the existing detection method, and has the characteristic of high detection efficiency.

Description

technical field [0001] The invention belongs to the technical field of fungal detection, relates to the detection of various fruit Phytophthora infestans, in particular to a detection method of Phytophthora infestans in fruits and an application thereof. Background technique [0002] Phytophthora de Bary (Phytophthora de Bary) is an important plant pathogenic fungus, which belongs to the kingdom Oomycetes, Oomycetes, Peronomycetes, and Pythiumaceae. Most pathogens of Phytophthora can cause devastating diseases of young annual plants, ornamental plants, fully mature fruit and forest trees. According to research reports, Phytophthora not only caused serious economic losses to the production of crops and cash crops, but also caused varying degrees of damage to forest vegetation around the world. Therefore, with the rapid development of international trade in agricultural products, the invasion of Phytophthora has seriously threatened my country's agricultural and forestry ecos...

Claims

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Application Information

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IPC IPC(8): C12Q1/6895C12Q1/686C12Q1/04
CPCC12Q1/6895C12Q1/686C12Q2521/301C12Q2565/125
Inventor 张文梅李明珠陈小玮张睿宁高峰王一鸣
Owner SHAANXI NORMAL UNIV
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