Preparation method and application of NDM-1 monoclonal antibody

A monoclonal antibody, NDM-1 technology, applied in the field of molecular biology, can solve the problems of low purity titer, poor specificity, and long time consumption, and achieve the effect of high purity, high sensitivity, and strong specificity
CN113150137AActive Publication Date: 2021-07-23深圳市龙华区疾病预防控制中心
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
深圳市龙华区疾病预防控制中心
Publication Date
2021-07-23

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Abstract

The invention discloses a preparation method and application of an NDM-1 monoclonal antibody. The preparation method comprises the following steps: synthesizing a Klebsiella pneumoniae NDM-1 gene, expressing and purifying NDM-1 protein, taking recombinant NDM-1 protein as immunogen, immunizing Balb / c mice, taking spleen cells of the immunized mice to fuse with myeloma NS-1 cells of the mice, screening hybridoma cells secreting specific McAb through an indirect ELISA method, inducing the mice to generate ascites by using a hybridoma cell strain, purifying antibodies by using protein G affinity chromatography to finally obtain seven NDM-1 protein monoclonal antibodies, and sequencing to obtain amino acid sequences of four monoclonal antibodies. A foundation is laid for establishing an NDM-1 detection method.
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Description

technical field

[0001] The invention relates to the field of molecular biology, in particular to a preparation method and application of an NDM-1 monoclonal antibody. Background technique

[0002] New Delhi metallo-beta-lactamase (NDM-1) is a newly discovered metalloenzyme (MBL) in 2008. It was named after it was first discovered in New Delhi, India. It was isolated from an Indian patient in Sweden. Carrier strains were Klebsiella pneumoniae and Escherichia coli. Subsequently, reports of NDM-1 drug-resistant bacteria appeared in more than 20 countries around the world. More and more data show that NDM-1-producing drug-resistant bacteria have spread globally and may become a threat to human health. The huge hidden dangers caused a global panic. According to reports, NDM-1 widely exists in Gram-negative bacilli, mainly distributed in Enterobacteriaceae and Acinetobacter bacteria, and has been found in Enterobacteriaceae Escherichia, Citrobacter, Salmonella NDM-1 resistant b...

Claims

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