Method for preparing acetylated sodium hyaluronate
A technology for hyaluronic acid and hyaluronic acid salt, applied in the field of preparing acetylated sodium hyaluronate, can solve the problems of long production cycle, environmental pollution by organic reagents, complicated production steps of acetylated hyaluronic acid, etc. The process is simple and easy to operate, and the effect of water retention and moisturizing effect is improved
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0023] 1) Prepare materials: prepare 1 g of potassium hyaluronate with a molecular weight of 10 kDa, 15 ml of formamide solvent, 5 ml of alkaline solution, 10 ml of acetic anhydride, and 5% sodium bicarbonate solution;
[0024] 2) Dissolving: Add the formamide solvent prepared in step 1) into a container, then add the sodium hyaluronate prepared in step 1) into the container containing the formamide solvent, stir at 30°C for 30 minutes to dissolve;
[0025] 3) Provide an alkaline environment: add the alkaline solution in step 1) into the container in step 2) and stir at 20°C for 30 minutes;
[0026] 4) Addition: Add the acetic anhydride in step 1) and stir in the container at 20°C for 24h;
[0027] 5) Neutralization: add 5% sodium bicarbonate solution in step 1) to the container of step 4), neutralize the excess acidic solution in the container, and continue stirring for 30 min;
[0028] 6) Water washing: wash the solution with a large amount of water to make the solution neu...
Embodiment 2
[0032] 1) Prepare materials: prepare 1g of sodium hyaluronate with a molecular weight of 3000kDa, 20ml of formamide solvent, 8ml of alkaline solution, 15ml of acetic anhydride, and 5% sodium bicarbonate solution;
[0033] 2) Dissolving: put the formamide solvent prepared in step 1) into a container, then add the sodium hyaluronate prepared in step 1) into the container containing the formamide solvent, and stir at 50°C for 50 minutes to dissolve;
[0034] 3) Provide an alkaline environment: add the alkaline solution in step 1) into the container and stir for 60 minutes at 25°C;
[0035] 4) Addition: Add the acetic anhydride in step 1) to the container at 25°C and stir for 24h;
[0036] 5) Neutralization: add 5% sodium bicarbonate solution in step 1) to the container of step 4), neutralize the excess acidic solution in the container, and continue stirring for 30 min;
[0037] 6) Water washing: wash the solution with a large amount of water to make the solution neutral;
[003...
Embodiment 3
[0041] 1) Prepare materials: prepare 1g of magnesium hyaluronate with a molecular weight of 130kDa, 25ml of formamide solvent, 10ml of alkaline solution, 20ml of acetic anhydride, and 5% sodium bicarbonate solution;
[0042] 2) Dissolving: put the formamide solvent prepared in step 1) into a container, then add the sodium hyaluronate prepared in step 1) into the container containing the formamide solvent, and stir at 60°C for 60 minutes to dissolve;
[0043] 3) Provide an alkaline environment: add the alkaline solution in step 1) into the container and stir at 40°C for 90 minutes;
[0044] 4) Addition: Add the acetic anhydride in step 1) to the container at 40°C and stir for 24h;
[0045] 5) Neutralization: add 5% sodium bicarbonate solution in step 1) to the container of step 4), neutralize the excessive acidic solution in the container and continue to stir for 30min;
[0046] 6) Water washing: wash the solution with a large amount of water to make the solution neutral;
[...
PUM
| Property | Measurement | Unit |
|---|---|---|
| molecular weight | aaaaa | aaaaa |
Abstract
Description
Claims
Application Information
Login to View More