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Preparation method and application of a gene recombinant cell based on MSC (mesenchymal stem cells)

A technology of genetic recombination, MSCD12235, applied in the field of preparation of genetically recombined cells, can solve the problem of not reaching the level of drug withdrawal

Active Publication Date: 2021-07-23
深圳明杰生物工程有限公司
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Problems solved by technology

The current status of using MSC to treat diabetes has the effect of improving complications to a certain extent, and can reduce the dosage of other hypoglycemic drugs, but it is still not enough to stop the drug

Method used

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  • Preparation method and application of a gene recombinant cell based on MSC (mesenchymal stem cells)
  • Preparation method and application of a gene recombinant cell based on MSC (mesenchymal stem cells)
  • Preparation method and application of a gene recombinant cell based on MSC (mesenchymal stem cells)

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Embodiment Construction

[0083] The following will clearly and completely describe the technical solutions in the embodiments of the present invention with reference to the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of them. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without creative efforts fall within the protection scope of the present invention.

[0084] It should be noted that all directional indications (such as up, down, left, right, front, back...) in the embodiments of the present invention are only used to explain the relationship between the components in a certain posture (as shown in the accompanying drawings). Relative positional relationship, movement conditions, etc., if the specific posture changes, the directional indication will also change accordingly.

[0085] In addition, the ...

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Abstract

The invention discloses a preparation method of a gene recombinant cell based on MSC (mesenchymal stem cells), which comprises the steps of: S101: respectively cloning coding genes of IL-35, IL-22 and FGF1 into an eukaryotic expression vector pDisplay with a transmembrane region structure to form cell membrane surface display plasmids (pDIL35, pDIL22 and pDFGF1) of the IL-35, the IL-22 and the FGF1; S102: preparing an hUC-MSC cell; and S103: equivalently mixingthe pDIL35 plasmid, the pDIL22 plasmid and the pDFGF1 plasmid and transfected into the MSC so as to generate an MSCd12235 cell. According to the invention, a plurality of cell factors with the known biological activity are expressed on the surface of one functional cell to form the gene recombinant cell with a novel biological function. The functional cell provides an immobilized action surface for the active factor, and the active factor endows the original cell with a new function and the functional cell is applied to treatment of diabetes mellitus.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a preparation method and application of MSC-based genetically recombined cells. Background technique [0002] Diabetes is a group of metabolic diseases characterized by hyperglycemia. It is also a common endocrine system disease. It is caused by the lack of insulin in the body, or the quality of insulin itself and other reasons that cause it to fail to play its normal physiological functions, resulting in the three major substances of sugar, fat, and protein mainly in sugar metabolism. A syndrome of metabolic disorders. The long-term high blood sugar in diabetes leads to chronic damage and dysfunction of various tissues, especially the eyes, kidneys, heart, blood vessels, and nerves. [0003] Diabetes is mainly divided into four types: type I diabetes (T1DM), type II diabetes (T2DM), gestational diabetes and special types of diabetes. [0004] In the stem cell therapy of diabetes,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/10C12N15/85C12N15/24C12N15/12A61K35/28A61K35/51A61P3/10
CPCC12N5/0665C12N15/85C07K14/54C07K14/501A61K35/28A61K35/51A61P3/10C12N2510/00
Inventor 张明杰马云坤
Owner 深圳明杰生物工程有限公司
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