Mycoplasma pneumoniae drug sensitivity rapid detection kit and preparation method thereof

Abstract

The invention discloses a mycoplasma pneumoniae drug sensitivity rapid detection kit and a preparation method thereof. The kit comprises mycoplasma pneumoniae culture solution, a drug sensitive plate and a drug for treating mycoplasma pneumoniae; the mycoplasma pneumoniae culture solution comprises the following components: 7 to 10 g of agar powder, 5 to 8 g of peptone, 2 to 3 g of short-chain peptide, 700 to 800 mg of morroniside, 3 to 5 g of yeast powder, 2 to 4 g of glucose, 2.5 to 3.5 g of sodium pyruvate, 90 to 120 mL of MEM culture medium, 2 mL of 1% (w / v) phenol red solution, 140 to 180 mL of horse serum, 300 thousand units of penicillin and 750 to 850 mL of distilled water; and the short-chain peptide is composed of 3 to 5 amino acid residues. The kit is stable in performance and high in specificity, and can accurately detect which antibiotic in various clinically common antibiotics is sensitive to a patient when the patient is infected with mycoplasma pneumoniae, so that medication is accurately guided, blind medication and excessive medication are avoided, and the recovery rate is increased.

Description

technical field [0001] The invention belongs to the technical field of clinical medical detection, and in particular relates to a rapid detection kit for drug sensitivity of Mycoplasma pneumoniae and a preparation method thereof. Background technique [0002] Mycoplasma pneumoniae is the causative agent of human mycoplasma pneumonia. Mycoplasma pneumonia is mainly transmitted through droplets. Children and adolescents are the most susceptible to infection. The incubation period can reach 2 to 3 weeks. It can occur in all seasons of the year, but it is more likely to be infected in autumn and winter. Therefore, it is of great significance to establish a sensitive and stable detection method for the detection, monitoring and epidemiological investigation of Mycoplasma pneumoniae. [0003] The incubation period of Mycoplasma pneumoniae infection is long, the disease develops slowly, and the symptoms are not obvious. They are similar to respiratory symptoms caused by common ba...

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Problems solved by technology

[0003] The incubation period of Mycoplasma pneumoniae infection is long, the disease develops slowly, and the symptoms are not obvious. It is similar to the respiratory symptoms caused by common bacterial and viral infections. Only by clinical symptoms and medication, it is impossible to accurately judge whether Mycoplasma pneumoniae infection has occurred

In addition, since mycoplasma has no cell wall, most antibiotics have no therapeutic effect on mycoplasma pneumoniae, and irregular drug use also makes a few effective antibiotics also have drug-resistant strains

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