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Gene composition, kit and method used for detecting pulmonary adenocarcinoma KRAS mutation

A technique for lung adenocarcinoma and composition is applied in the field of gene composition for detecting KRAS mutation of lung adenocarcinoma, which can solve the problems of high cost, low accuracy, cumbersome process and the like, and achieves high accuracy, good sensitivity and specificity, Easy-to-use effects

Active Publication Date: 2021-07-23
ZHONGSHAN HOSPITAL FUDAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Currently, direct clinical detection of KRAS mutations is costly, cumbersome, and low in accuracy, and detection methods with high sensitivity, specificity, and application value are still needed as a reference for formulating individualized targeted therapy plans for patients with lung adenocarcinoma

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  • Gene composition, kit and method used for detecting pulmonary adenocarcinoma KRAS mutation

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Effect test

Embodiment 1

[0028] Example 1: Sensitivity and specificity of gene composition for detecting KRAS mutation in lung adenocarcinoma

[0029] To construct a scoring model for detecting KRAS mutations in lung adenocarcinoma, the steps are as follows:

[0030] 1. The gene expression data of 510 cases of lung adenocarcinoma samples were obtained from the TCGA database, which were divided into KRAS mutation, EGFR mutation and wild group, and the differential genes of the three groups were screened out through the R language limma package.

[0031] 2. Select the 56 genes with the strongest correlation with KRAS mutation among the modules most related to KRAS gene mutation through WGCNA.

[0032] 3. Screen 25 genes by Lasso regression: ELN, PODN, LAMC3, COL5A3, CRYAB, SOX18, AKT3, FAM101B, TIE1, SH3PXD2A, EXOC3L2, NOTCH1, NDST1, MMRN2, SH3RF3, ZMIZ1, RNF122, FLT4, F10, TLN1 , SOX17, PIP5K1C, TMEM255B, WSCD1, PCDHB3.

[0033] 4. Determine the threshold value of each gene through the ROC curve, as ...

Embodiment 2

[0041] Embodiment 2 clinical trials

[0042] 47 samples of lung adenocarcinoma were collected from the Thoracic Surgery Department of Zhongshan Hospital affiliated to Fudan University for gene sequencing, and the gene expression data were divided into two groups according to the Score scoring model. The cut-off value of cancer is non-KRAS mutation group, and its sensitivity and specificity are shown in Table 3.

[0043] table 3

[0044] Clinical detection of KRAS mutation+ Clinical detection of KRAS mutations - Predicted KRAS mutation+ 9 4 Predicted KRAS mutation- 1 33

[0045] It can be seen from Table 3 that the sensitivity is 0.90 and the specificity is 0.89. Clinical trials have verified that the lung adenocarcinoma KRAS mutation scoring model constructed by the genetic composition of the present invention has high accuracy, good sensitivity and specificity for detecting lung adenocarcinoma KRAS mutations .

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Abstract

The invention discloses a gene composition, a kit and a method used for detecting pulmonary adenocarcinoma KRAS mutation, and belongs to the technical field of molecular diagnosis. The gene composition consists of the following 25 genes, including ELN, PODN, LAMC3, COL5A3, CRYAB, SOX18, AKT3, FAM101B, TIE1, SH3PXD2A, EXOC3L2, NOTCH1, NDST1, MMRN2, SH3RF3, ZMIZ1, RNF122, FLT4, F10, TLN1, SOX17, PIP5K1C, TMEM255B, WSCD1 and PCDHB3. In the invention, through the above gene composition, a Score scoring model is constructed for detecting the pulmonary adenocarcinoma KRAS mutation, and therefore, the gene composition has the advantages of high accuracy and good sensitivity and specificity, is convenient in operation, can carry out quantitative analysis and has a wide application prospect.

Description

technical field [0001] The invention belongs to the technical field of molecular diagnosis, and in particular relates to a gene composition, a kit and a method for detecting KRAS mutation of lung adenocarcinoma. Background technique [0002] As the most common and deadly tumor, lung cancer ranks first among all tumors in terms of morbidity and mortality. Lung adenocarcinoma is the most common subtype of lung cancer, accounting for more than 40% of lung cancer cases. Kirsten murine sarcoma virus oncogene (Kirstenrat sarcoma viral oncogene, KRAS) is the most important gene in the RAS family, and KRAS mutation is one of the common carcinogenic factors of lung adenocarcinoma, and the mutation rate in lung cancer is 32%. Once KRAS is mutated, it will lose the activity of GTP hydrolase, and then continue to activate, which will promote the continuous proliferation of cells and become cancerous. The most common way of KRAS gene mutation is point mutation, common mutation forms are...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6886C12Q1/6851C12N15/11
CPCC12Q1/6886C12Q1/6851C12Q2600/156C12Q2600/158C12Q2537/165
Inventor 卞赟艺毕国澍梁嘉琪郑元生单光耀薛亮张毅姚光宇詹成范虹
Owner ZHONGSHAN HOSPITAL FUDAN UNIV
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