InDel molecular marker closely linked with major QTLPm3.1 for powdery mildew resistance in bitter gourd and application

Abstract

The invention discloses an InDel molecular marker closely linked with a major QTLPm3.1 for powdery mildew resistance in bitter gourd. The molecular marker is InDel0362 and has a base sequence as shown in SEQ. ID. NO.1 in a sequence table. The major QTLPm3.1 for powdery mildew resistance in bitter gourd and the InDel molecular marker are both located on chromosome 3 of bitter gourd, the genetic distance of the major QTLPm3.1 for powdery mildew resistance in bitter gourd is 14 cM, and the physical distance is 2.9 Mb. Therefore, a primer pair for amplifying the InDel molecular marker and a kit thereof are also developed. The InDel molecular marker can be used for simple, rapid and high-throughput detection of powdery mildew resistance in bitter gourd, which can accelerate the excavation and utilization of key genes of powdery mildew resistance and promote molecular breeding of powdery mildew resistance in bitter gourd. Researches show that the identification result of the molecular marker is consistent with the field inoculation identification result, and molecular marker-assisted selection can be carried out on bitter gourd breeding materials before powdery mildew in the seedling stage.

Description

technical field [0001] The invention belongs to the field of molecular biology, and in particular relates to an InDel molecular marker closely linked with the main effect QTL Pm3.1 of bitter gourd powdery mildew resistance and its application. Background technique [0002] Bitter melon (Momordica charantia L.) is an annual climbing herb belonging to Cucurbitaceae Momordica charantia, which has high nutritional value and therapeutic effect. In recent years, the planting area of ​​bitter gourd has been increasing continuously, and it has become the main vegetable variety and characteristic industry of "South Vegetable Transport North". Powdery mildew is a worldwide disease of melon vegetables, which can occur from seedling stage to harvest stage. In recent years, with the increase in the planting area and large-scale development of melon vegetables, powdery mildew has become one of the main diseases that cause yield reduction and fruit quality decline, and hinder green produc...

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Problems solved by technology

[0007] However, the research on the fine mapping of the main QTL for powdery mildew of bitter gourd and its related molecular markers is still incomplete.

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