LAMP detection method for influenza H3N2 and hand-foot-and-mouth disease CVA6

Abstract

The invention discloses an LAMP (loop-mediated isothermal amplification) detection method for influenza H3N2 and hand-foot-and-mouth disease CVA6, relates to the technical field of virus detection, and comprises the following steps: step A, designing LAMP primer groups for the influenza H3N2 and the hand-foot-and-mouth disease CVA6; step B, screening an effective LAMP primer group of tge influenza H3N2 and tge hand-foot-mouth virus CVA6; and step C, evaluating the effective LAMP primer group by adopting multiple samples, and carrying out LAMP detection experiment. The RT-LAMP method for detecting the influenza H3N2 and the hand-foot-and-mouth disease CVA6 has feasibility, compared with a PCR amplification detection method, the RT-LAMP method is low in instrument and equipment requirement, easy and convenient to operate and short in consumed time, has the potential of developing rapid detection, and in addition, in multi-sample screening, the RT-LAMP method also has high positive detection rate, which comprehensively improves the prevention and control capability of infectious diseases.

Description

technical field [0001] The invention relates to the technical field of virus detection, in particular to a LAMP detection method for influenza H3N2 and hand, foot and mouth disease CVA6. Background technique [0002] Influenza Virus (Influenza Virus) is a single-stranded negative-sense RNA virus that can infect humans and animals. According to the antigenicity of viral nucleoprotein and matrix protein, influenza virus is divided into 4 types, A, B, C, and D Virus. The antigens of influenza B, C and D viruses are very stable and seldom mutate, but the surface antigens hemagglutinin (HA) and neuraminidase (Neuraminidase, NA) on the surface of influenza A virus are easy to mutate and produce Many subtypes. Currently known HA has 18 subtypes (H1-H18), NA has 11 subtypes (N1-N11), and random combinations of HA and NA can form various subtypes. Due to the strong variability of the virus antigen and the rapid spread, it has repeatedly caused worldwide pandemics and seriously end...

AI Technical Summary

Problems solved by technology

[0013] The diagnosis of hand, foot and mouth disease needs to rely on the PCR method to detect viral nucleic acid. However, due to the limitations of equipment and technology in primary hospitals, the PCR method is more complicated to operate, requires professional PCR equipment, and takes a long time, making it difficult to popularize.

[0014] Due to the high sensitivity and specificity of the PCR method, in the process of extracting and amplifying nucleic acids, nucleic acid products are likely to contaminate reagents, equipment and ventilation systems in the laboratory, resulting in laboratory contamination and false positive results.

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