Breeding method for high-yield disease-resistant strong gluten wheat in middle and lower reaches of Yangtze River
A high-yield, disease-resistant, mid- and downstream technology, applied in the fields of botanical equipment and methods, biochemical equipment and methods, plant genetic improvement, etc. Problems such as lack of points and large segregation of offspring traits
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Embodiment 1
[0047] Embodiment 1, a kind of breeding method of high-yielding, disease-resistant and strong-gluten wheat in the middle and lower reaches of the Yangtze River
[0048] Parental selection: most of the main varieties with excellent comprehensive agronomic traits and good comprehensive disease resistance in the middle and lower reaches of the Yangtze River and their derived varieties (lines) were screened for the scab resistance locus QFhs.crc-2DL, and Yangmai 158 As a positive control, Yangmai 17 was selected as parent 1.
[0049] 1) Planted in January 2006, from April to May, the wheat variety Yangmai 17 carrying the scab resistance locus QFhs.crc-2DL in the middle and lower reaches of the Yangtze River was used as the male parent, and the intermediate material "Amigo" resistant to powdery mildew was selected As the female parent, hybridize in the greenhouse, and harvest the hybrid F1 at the end of June;
[0050] Amig is a powdery mildew resistant line (References: Zhang Xu, ...
Embodiment 2
[0065] Example 2. Establishment of molecular marker detection method for scab resistance locus QFhs.crc-2DL
[0066] The primer set of molecular marker Xgwm539 was used to detect whether Yangmai 17, leaves of F3 single plant, and leaves of F4 mixed row carried the disease-resistant genotype of QFhs.crc-2DL, the scab resistance site.
[0067] 1. Genomic DNA was extracted from the leaves of Yangmai 17, individual F3 plants, and mixed leaves of F4 lines by CTAB method, and was diluted to obtain a template solution with a DNA concentration of about 30 ng / μL.
[0068] 2. Using the genomic DNA extracted in step 1 as a template, the scab resistance marker Xgwm539 for detecting wheat described in Example 1 was used for PCR amplification to obtain the amplified product.
[0069] The primer sequences used in this study to identify the scab resistance locus QFhs.crc-2DL are shown in Table 2:
[0070] Table 2 Sequence information of QFhb-YM17 linked marker primers
[0071]
[0072] T...
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