Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Breeding method for high-yield disease-resistant strong gluten wheat in middle and lower reaches of Yangtze River

A high-yield, disease-resistant, mid- and downstream technology, applied in the fields of botanical equipment and methods, biochemical equipment and methods, plant genetic improvement, etc. Problems such as lack of points and large segregation of offspring traits

Active Publication Date: 2021-07-30
JIANGSU LIXIAHE REGION AGRI RES INST
View PDF6 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0010] (10) In summary, the existing problems in the prior art are: lack of germplasm resources and genes / loci for excellent disease resistance or strong gluten, long breeding cycle, unreliable selection of low-generation phenotypes, and large segregation of offspring traits , the target traits of breeding selection are difficult to stabilize in a short period of time, and the workload of disease resistance identification and yield identification of the advanced generation is relatively large, which is not conducive to artificial selection, and the effect is difficult to achieve the expected goal

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Breeding method for high-yield disease-resistant strong gluten wheat in middle and lower reaches of Yangtze River
  • Breeding method for high-yield disease-resistant strong gluten wheat in middle and lower reaches of Yangtze River
  • Breeding method for high-yield disease-resistant strong gluten wheat in middle and lower reaches of Yangtze River

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] Embodiment 1, a kind of breeding method of high-yielding, disease-resistant and strong-gluten wheat in the middle and lower reaches of the Yangtze River

[0048] Parental selection: most of the main varieties with excellent comprehensive agronomic traits and good comprehensive disease resistance in the middle and lower reaches of the Yangtze River and their derived varieties (lines) were screened for the scab resistance locus QFhs.crc-2DL, and Yangmai 158 As a positive control, Yangmai 17 was selected as parent 1.

[0049] 1) Planted in January 2006, from April to May, the wheat variety Yangmai 17 carrying the scab resistance locus QFhs.crc-2DL in the middle and lower reaches of the Yangtze River was used as the male parent, and the intermediate material "Amigo" resistant to powdery mildew was selected As the female parent, hybridize in the greenhouse, and harvest the hybrid F1 at the end of June;

[0050] Amig is a powdery mildew resistant line (References: Zhang Xu, ...

Embodiment 2

[0065] Example 2. Establishment of molecular marker detection method for scab resistance locus QFhs.crc-2DL

[0066] The primer set of molecular marker Xgwm539 was used to detect whether Yangmai 17, leaves of F3 single plant, and leaves of F4 mixed row carried the disease-resistant genotype of QFhs.crc-2DL, the scab resistance site.

[0067] 1. Genomic DNA was extracted from the leaves of Yangmai 17, individual F3 plants, and mixed leaves of F4 lines by CTAB method, and was diluted to obtain a template solution with a DNA concentration of about 30 ng / μL.

[0068] 2. Using the genomic DNA extracted in step 1 as a template, the scab resistance marker Xgwm539 for detecting wheat described in Example 1 was used for PCR amplification to obtain the amplified product.

[0069] The primer sequences used in this study to identify the scab resistance locus QFhs.crc-2DL are shown in Table 2:

[0070] Table 2 Sequence information of QFhb-YM17 linked marker primers

[0071]

[0072] T...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a breeding method for high-yield disease-resistant strong gluten wheat in the middle and lower reaches of Yangtze River. The breeding method comprises the following steps: a wheat variety which has excellent comprehensive agronomic characters and good comprehensive disease resistance and carries a gibberellic disease-resistant site QFhs.crc-2DL in the middle and lower reaches of Yangtze River is taken as a parent 1, a powdery mildew-resistant wheat variety (line) or an intermediate material is taken as a parent 2, after the parent 2 and the parent 1 are hybridized once, backcrossing is carried out for multiple times to fix the genetic background of excellent comprehensive agronomic characters and good comprehensive disease resistance of offspring, in the backcrossing process, each generation is artificially inoculated to identify powdery mildew resistance, a powdery mildew-resistant offspring single plant is hybridized with a powdery mildew-resistant and high-yield strong gluten or medium strong gluten wheat variety (line) parent 3 in the middle and lower reaches of the Yangtze River, and the high-yield disease-resistant strong gluten wheat variety in the middle and lower reaches of the Yangtze River is bred by combining the selection of a gibberellic disease-resistant molecular marker, the phenotype selection for enhancing comprehensive disease resistance, the high-generation identification of yield and the evaluation of the quality of flour and bread. The method greatly reduces the workload of identification of low-generation and high-generation field gibberellic disease.

Description

technical field [0001] The invention belongs to the technical field of wheat breeding methods and relates to a method for breeding high-yield, disease-resistant, strong-gluten wheat in the middle and lower reaches of the Yangtze River. Background technique [0002] (1) The middle and lower reaches of the Yangtze River is the largest rice-wheat rotation area in my country, and the total output of rice and wheat accounts for more than 1 / 3 of the country's total. Since the 1990s, with the continuous expansion of the area of ​​rice machine transplanting and direct seeding, the harvest period has been continuously delayed, resulting in a gradual delay in the wheat sowing period. Delayed sowing causes wheat to be prone to high temperature and humidity from flowering stage to maturity stage, which increases the risk of encountering various diseases and adversity: if there is a large outbreak of head blight, it will not only seriously affect the yield and quality of wheat, but the t...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/02A01H1/04A01H6/46C12Q1/6895
CPCA01H1/02A01H1/04A01H6/4678C12Q1/6895C12Q2600/13C12Q2600/156C12Q2600/166C12Q2600/172
Inventor 胡文静高德荣吴荣林张勇陆成彬吴宏亚
Owner JIANGSU LIXIAHE REGION AGRI RES INST
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products