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A breeding method for high-yield, disease-resistant and strong-gluten wheat in the middle and lower reaches of the Yangtze River

A high-yield, disease-resistant, mid- and downstream technology, applied in the fields of botanical equipment and methods, biochemical equipment and methods, and plant genetic improvement, can solve the problems of long breeding cycle, stability, and large separation of progeny traits, and accelerate the breeding process. , Shorten the breeding years, the effect of comprehensive disease resistance is good

Active Publication Date: 2022-07-29
JIANGSU LIXIAHE REGION AGRI RES INST
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0010] (10) In summary, the existing problems in the prior art are: lack of germplasm resources and genes / loci for excellent disease resistance or strong gluten, long breeding cycle, unreliable selection of low-generation phenotypes, and large segregation of offspring traits , the target traits of breeding selection are difficult to stabilize in a short period of time, and the workload of disease resistance identification and yield identification of the advanced generation is relatively large, which is not conducive to artificial selection, and the effect is difficult to achieve the expected goal

Method used

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  • A breeding method for high-yield, disease-resistant and strong-gluten wheat in the middle and lower reaches of the Yangtze River
  • A breeding method for high-yield, disease-resistant and strong-gluten wheat in the middle and lower reaches of the Yangtze River
  • A breeding method for high-yield, disease-resistant and strong-gluten wheat in the middle and lower reaches of the Yangtze River

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Effect test

Embodiment 1

[0047] Embodiment 1, a kind of high-yield, disease-resistant and strong-gluten wheat breeding method in the middle and lower reaches of the Yangtze River

[0048] Parental selection: Most of the main cultivars with excellent comprehensive agronomic traits and good comprehensive disease resistance and their derived cultivars (lines) in the middle and lower reaches of the Yangtze River were screened for the scab resistance locus QFhs.crc-2DL. As a positive control, Yangmai 17 was screened as parent 1.

[0049] 1) Planted in January 2006, and from April to May, the wheat variety Yangmai 17 carrying the scab resistant locus QFhs.crc-2DL in the middle and lower reaches of the Yangtze River was used as the male parent, and the intermediate material "Amigo" resistant to powdery mildew was selected As the female parent, hybridize in the greenhouse and harvest the hybrid F1 at the end of June;

[0050] Amig is a powdery mildew resistant strain (References: Zhang Xu, Zang Yuhui, Liu Zh...

Embodiment 2

[0065] Example 2. Establishment of Molecular Markers to Detect the Anti-Gibberella Disease Site QFhs.crc-2DL Method

[0066] The primer set of molecular marker Xgwm539 was used to detect whether Yangmai 17, leaves of F3 single plant, and mixed leaves of F4 lines carried the resistance genotype QFhs.crc-2DL.

[0067] 1. The genomic DNA of Yangmai 17, F3 individual leaves, and F4 mixed leaves were extracted by CTAB method, and the template solution with DNA concentration of about 30ng / μL was obtained by dilution.

[0068] 2. Using the genomic DNA extracted in step 1 as a template, PCR amplification was carried out using Xgwm539, an anti-head blight marker for detecting wheat as described in Example 1, to obtain an amplification product.

[0069] The primer sequences used in this study to identify the scab-resistant locus QFhs.crc-2DL are shown in Table 2:

[0070] Table 2 QFhb-YM17 linked marker primer sequence information

[0071]

[0072] A PCR amplification method was us...

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Abstract

The invention discloses a high-yield, disease-resistant and strong-gluten wheat breeding method for the middle and lower reaches of the Yangtze River. The wheat variety is parent 1, and the powdery mildew resistant wheat variety (line) or intermediate material is used as parent 2. Parent 2 is crossed with parent 1 once and then backcrossed for several times to fix the offspring. The comprehensive agronomic characters are good and the comprehensive disease resistance is good. In the process of backcrossing, each generation of artificial inoculation was used to identify powdery mildew resistance, and the powdery mildew-resistant progeny were crossed with the parents of the strong-gluten or medium-strong gluten wheat varieties (lines) with high yield and resistance to powdery mildew in the middle and lower reaches of the Yangtze River. Combined with the selection of molecular markers for resistance to head blight, the phenotypic selection of comprehensive disease resistance will be strengthened, the yield of high-generation, flour and bread quality will be evaluated, and high-yield, disease-resistant and strong-gluten wheat varieties in the middle and lower reaches of the Yangtze River will be bred. This method greatly reduces the workload of identification of low- and high-generation field head blight.

Description

technical field [0001] The invention belongs to the technical field of wheat breeding methods, and relates to a high-yield, disease-resistant and strong-gluten wheat breeding method in the middle and lower reaches of the Yangtze River. Background technique [0002] (1) The middle and lower reaches of the Yangtze River are the largest rice-wheat rotation areas in my country, and the total output of rice and wheat accounts for more than 1 / 3 of the country's total. Since the 1990s, with the continuous expansion of the area of ​​rice machine transplanting and direct seeding, the harvest period has been continuously delayed, resulting in the gradual delay of the wheat sowing period. Delayed sowing makes wheat prone to high temperature and humidity from flowering to maturity, increasing the risk of encountering various diseases and adversity: if there is a big outbreak of scab, it will not only seriously affect the yield and quality of wheat, but also the toxin DON will be harmful...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6895A01H1/02A01H1/04A01H6/46
CPCA01H1/02A01H1/04A01H6/4678C12Q1/6895C12Q2600/13C12Q2600/156C12Q2600/166C12Q2600/172
Inventor 胡文静高德荣吴荣林张勇陆成彬吴宏亚
Owner JIANGSU LIXIAHE REGION AGRI RES INST
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