Selective breeding method of cladosporium cucumerinum and corynespora cassiicola resistant sicyos cucumber hybrids

Abstract

The invention discloses a selective breeding method of cladosporium cucumerinum and corynespora cassiicola resistant sicyos cucumber hybrids, and cladosporium cucumerinum resistant InDel molecular marker auxiliary selection. A cladosporium cucumerinum and corynespora cassiicola resistant sicyos pure line obtained through multi-generation backcrossing transferring breeding is female parent, and thecorynespora cassiicola resistant excellent inbred line based on conventional breeding inbred homozygosity is a male parent, so that the cladosporium cucumerinum and corynespora cassiicola resistant sicyos cucumber hybrids is obtained through crossbreeding. According to the method disclosed by the invention, sicyos cucumber hybrid I new varieties with high cladosporium cucumerinum and corynesporacassiicola resistance, low temperature resistance, poor light resistance, wide adaptability, excellent comprehensive features and high yield are selectively bred.

Description

Technical field [0001] The invention belongs to the technical field of plant breeding, and specifically relates to a method for selecting and breeding dense thorn cucumber hybrids resistant to scab and coryneform leaf spot. Background technique [0002] Cucumber (Cucumis sativus L.) is an important economic vegetable crop cultivated on a global scale. my country is the country with the largest cucumber cultivation area and the highest total yield in the world, and the protected area cultivation area ranks first among all kinds of vegetables. Cucumber scab, commonly known as "gum disease" or "scab disease", is a low-temperature, devastating disease that seriously harms cucumber production in Europe, North America, Southeast Asia and other places. It began to occur in my country in the late 1980s. It was listed as a quarantine disease in 1991. The disease is prone to epidemics and disasters in protected areas. It can occur during the entire growth period. It can infect any part exc...

AI Technical Summary

Problems solved by technology

When the traditional breeding method is used to introduce the scab resistance gene into the recurrent parent to breed the inbred line, it is necessary to manually inoculate and identify the individual plants of the backcross population to select the scab resistant individual plants and continue to backcross until the backcross multi-generation inheritance The background is basically the same as that of the recurrent parent, and the scab-resistant inbred line with stable traits can be obtained after selfing for 2-3 generations; since this trait is controlled by a dominant single gene, segregation will occur in the selfing generation, and the heterozygous strains will show resistance to scab. disease, heterozygous disease-resistant strains and homozygous disease-resistant strains cannot be distinguished by artificial inoculation identification methods

Moreover, the traditional backcross breeding method requires professional plant protection personnel and breeding experts, and the workload is heavy, and the selection is inaccurate

It can be seen that using the conventional backcross transfer method, for the traits controlled by a dominant single gene, the last selfed generation needs to use the artificial inoculation identification method of the seedling stage phenotype, and at the same time, it needs 2-3 generations of selfing and inoculation to achieve the target trait. Basically accurate selection, long breeding time, poor accuracy and low efficiency

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