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Biocontrol bacterial strain g68 for preventing and controlling plant diseases and preparation method and application thereof

A technology for plant disease control and bio-control strains, applied in the field of agricultural biology, can solve problems such as environmental pollution and pesticide residues, and achieve the effects of reducing production costs, broad antibacterial spectrum, and reducing pesticide residues

Active Publication Date: 2017-04-05
INST OF AGRI RESOURCES & ENVIRONMENT GUANGDONG ACADEMY OF AGRI SCI
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  • Abstract
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In order to solve the above problems, the present invention provides a kind of high-efficiency, broad-spectrum, stable effect and environment-friendly plant disease biocontrol bacteria, aiming at the large-scale use of chemical fungicides in the prevention and control of plant diseases in agricultural production, resulting in pesticide residues and environmental pollution. Strain G68

Method used

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  • Biocontrol bacterial strain g68 for preventing and controlling plant diseases and preparation method and application thereof
  • Biocontrol bacterial strain g68 for preventing and controlling plant diseases and preparation method and application thereof
  • Biocontrol bacterial strain g68 for preventing and controlling plant diseases and preparation method and application thereof

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Embodiment 1

[0024] 1. Isolation, screening and identification of biocontrol strain G68

[0025] A plant disease biocontrol strain G68, named Bacillus subtilis G68, was isolated from the compost of the organic solid waste treatment base of the Institute of Agricultural Resources and Environment, Guangdong Academy of Agricultural Sciences. The specific separation, screening and identification methods are as follows:

[0026] (1) Isolation of biocontrol strain G68

[0027] Weigh 10.0 g of evenly mixed compost sample, add 90 ml of sterile water, shake for 30 min at 30°C, 180 r / min, and dilute the bacterial solution by 10 -1 、10 -2 、10 -3 、10 -4 、10 -5 、10 -6 、10 -7 times, draw the appropriate diluted bacterial suspension (diluted 10 -5 、10 -6 、10 -7 times the bacterial solution) 100 µl was evenly spread on the nutrient agar plate, each dilution was repeated three times, and cultured in a constant temperature incubator at 30°C for 1-2 days. According to the different physiological ...

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Abstract

The invention discloses a biocontrol strain G68 for preventing and treating plant diseases, and a preparation method and application of a microbial inoculant thereof. The biocontrol strain is named Bacillus subtilis G68, and the collection number is CGMCC No.8978. The preparation method of the biocontrol strain microbial inoculant comprises the following steps: inoculating the activated strain G68 in an NYBE culture fluid, culturing for 22-26 hours to obtain a seed fluid, inoculating the seed fluid in an NYBE fermentation medium containing 0.08-0.12 mM MnSO4, and culturing for 70-74 hours to obtain a fermentation liquid which is the biocontrol strain microbial inoculant. The biocontrol microbial inoculant containing the strain G68 can promote the germination of plant seeds and accelerate the plant growth, and the prevention effects for seedling blight and early blight of tomato respectively reach 53.8% and 60.1%. The strain G68 has the characteristics of wide antimicrobial spectrum, stable effect, low preparation cost, simple application method, environment friendliness, no toxicity or harm and the like, and has favorable application prospects in the aspect of prevention and treatment of plant diseases.

Description

technical field [0001] The invention belongs to the field of agricultural biotechnology, and in particular relates to a biocontrol bacterium for preventing and controlling plant diseases and a preparation method and application of the bacterium agent. Background technique [0002] As a large agricultural country in my country, the problem of crop disease control is very important in agricultural planting. According to reports, at least 10% to 15% of my country's food crops are lost each year due to disease problems. The occurrence of crop diseases often causes serious losses to the national economy and people's lives. Plant disease control methods include plant quarantine, disease resistance breeding, agricultural control, chemical control, physical and mechanical control, and biological control. [0003] The method of selection and utilization of disease-resistant breeding is an effective way to prevent and control the occurrence of diseases. However, disease-resistant v...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01N63/00A01P3/00A01P1/00C12R1/125
Inventor 顾文杰卢钰升杨少海解开治李文英蒋瑞萍孙丽丽
Owner INST OF AGRI RESOURCES & ENVIRONMENT GUANGDONG ACADEMY OF AGRI SCI
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