Automatic separation device for extracellular vesicles

An automatic separation and vesicle technology, applied in biochemical cleaning devices, enzymology/microbiology devices, stress-stimulated microbial growth methods, etc., can solve the problems of difficult elution and separation of extracellular vesicles, expensive antibodies, etc. Achieve automatic separation of extracellular vesicles, improve collection efficiency, and enhance the activity of extracellular vesicles

Pending Publication Date: 2021-07-30
上海富劢生物技术有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the extracellular vesicles obtained by this method are of high purity, the extracellular vesicles bound by immunomagnetic beads are not easy to be eluted and separated, and the antibody is expensive, so it is not suitable for the enrichment of a large number of extracellular vesicles

Method used

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  • Automatic separation device for extracellular vesicles
  • Automatic separation device for extracellular vesicles
  • Automatic separation device for extracellular vesicles

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Embodiment Construction

[0023] The following description serves to disclose the present invention to enable those skilled in the art to carry out the present invention. The preferred embodiments described below are only examples, and those skilled in the art can devise other obvious variations. The basic principles of the present invention defined in the following description can be applied to other embodiments, variations, improvements, equivalents and other technical solutions without departing from the spirit and scope of the present invention.

[0024] Those skilled in the art should understand that in the disclosure of the present invention, the terms "vertical", "transverse", "upper", "lower", "front", "rear", "left", "right", " The orientation or positional relationship indicated by "vertical", "horizontal", "top", "bottom", "inner", "outer", etc. is based on the orientation or positional relationship shown in the drawings, which are only for the convenience of describing the present invention...

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Abstract

The invention provides an automatic separation device for biological and plant extracellular vesicles. The automatic separation device comprises an incubation container, an elution container, a manipulator assembly and a tool assembly, the tool assembly comprises a configuration frame, a stirring part and a magnetic part, the stirring part and the magnetic part are arranged on the configuration frame, the incubation container and the elution container are located around the configuration frame, the incubation container accommodates a sample and adsorbs magnetic beads of extracellular vesicles in the sample, the manipulator assembly can grab the stirring part and the magnetic part and move among the configuration frame, the incubation container and the elution container, the stirring part applies stirring force to the incubation container and the elution container, and the magnetic part adsorbs magnetic beads with extracellular vesicles in the incubation unit and transfers and releases the magnetic beads to the elution unit, so that the elution container collects the extracellular vesicles, the extracellular vesicles are automatically separated, and the collection efficiency is effectively improved.

Description

technical field [0001] The invention relates to the field of biological detection equipment, in particular to an automatic separation device for extracellular vesicles. Background technique [0002] Extracellular vesicles (EVs) are small vesicles secreted by cells with a diameter of 30-200 nm that are shed from the membrane when cells are activated, injured or apoptotic. Contains a variety of RNA, cholesterol, and protein substances, which can be actively secreted by various cells such as immune cells, stem cells, cardiovascular cells, and tumor cells, and participate in processes such as cell communication, cell migration, and tumor growth. Because extracellular vesicles reflect the physiological and functional state of their parental cells, have high abundance in blood, urine, saliva, ascites and other body fluids, and are easy to obtain non-invasively, they play an important role in disease diagnosis, treatment and prognosis. Therefore, it is of great significance to the...

Claims

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Application Information

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IPC IPC(8): C12M1/42C12M1/02C12M1/00
CPCC12M23/48C12M27/02C12M29/06C12M35/06
Inventor 刘冠宏
Owner 上海富劢生物技术有限公司
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