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Protein chip for detecting coronavirus, preparation method of protein chip and detection kit

A detection kit and coronavirus technology, applied in the biological field, can solve the problems of increasing the difficulty of detection, increasing the affinity of RBD and human ACE2, and achieving the effect of low sample consumption

Inactive Publication Date: 2021-07-30
RAYBIOTECH INC GUANGZHOU +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The study found that these mutations may increase the affinity of RBD and human ACE2, thereby increasing the difficulty of detection

Method used

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  • Protein chip for detecting coronavirus, preparation method of protein chip and detection kit
  • Protein chip for detecting coronavirus, preparation method of protein chip and detection kit
  • Protein chip for detecting coronavirus, preparation method of protein chip and detection kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0120] A protein chip detecting a new crown virus (SARS-COV-2), the chip antigen as a polypeptide fragment in different regions of Sars-Cov-2Spike protein, the polypeptide fragment, and the human IgG or IgM specific binding and is selected from The polypeptide fragment sequence in Table 1 above, the polypeptide fragment is arranged on the slide, as shown in Table 2 above, and the protein chip is prepared by:

[0121] The chip antigen package is arranged and arranged on the carrier: a polypeptide fragment of the crown SARS-COV-2Spike protein that can be arranged with a package of the human IgG or IgM-specific binding to form a protein microarray chip on the carrier; The package was bsa: 0.4 g, mannitol: 2.5 g, trehalose: 10g, glycine: 0.3 g, PRO-Clin300: 0.1 g, 0.01M PBS: 80g, the pH of the package was adjusted to 9.0; 0.01M PBS is added to 100 g;

[0122] Dry: The protein microarray chip is placed in a vacuum degree of -0.09Pa, the temperature of <30 ° C, humidity <30% is dried in...

Embodiment 2

[0126] The protein chip of the SARS virus is detected, wherein the chip antigen is a polypeptide fragment in different regions of the SARS viral SpiKe protein, which can be specifically bind to human IgG or IgM, and the polypeptide fragment is arranged on the slide, such as Table 2 Design, the protein chip preparation method is the same as in Example 1; wherein the difference is that the package is bsa: 0.2 g, mannitol: 1g, trehalose: 5g, glycine: 0.5g, PRO-Clin300: 0.1 g, 0.01 M PBS: 80g, the pH of the package was adjusted to 9.0; 0.01 M PBS was added to 100 g.

Embodiment 3

[0128] The protein chip of the MERS virus is detected, wherein the chip antigen is a polypeptide fragment in different regions of the MERS viral SpiKe protein, which can be bonded to human IgG or IgM, and the polypeptide fragment is arranged on a slide such as Table 2 above. As shown, the protein chip preparation method and conditions are the same as in Example 1.

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Abstract

The invention discloses a protein chip for detecting coronavirus. Theprotein chipcomprises a carrier and chip antigens, wherein the chip antigens are polypeptide fragments in different regions in coronavirus Spike protein, and the polypeptide fragments are arranged on the carrier to form a protein microarray chip. The invention further discloses a preparation method of the protein chip for detecting the coronavirus and a coronavirus detection kit. According to the invention, polypeptide fragments in different regions of Spike protein are fixed on a carrier, so that high-throughput screening of a plurality of targets in one-time determination is realized, and the protein chip can be used for detecting a plurality of coronaviruses.

Description

Technical field [0001] The present invention relates to the field of biotechnology, and more particularly to a protein chip detecting a coronavirus and a preparation method thereof and a detection kit. Background technique [0002] In the 21st century, infectious diseases caused by coronaviruses have emerged. For example, Severe Acute RespiratorySyndrome-Coronavirus, Sars-CoVs caused by atwy-type pneumonia and the 2012 Middle Eastern respiratory syndrome-Coronavirus, Mers-CoVs, the Middle East respiratory syndrome, It causes about 10% and 36% of the mortality. [0003] The coronary virus family is a virus, coronavirus. The family can be further subdivided into α, β, γ and Δ4. SARS-COV-2 belongs to the beta genome, and its genomic sequence is 79.5% of Similarity with SARS-COV, 40% similarity with MERS-COV, with Bat-sl-Covzc45 more than 85 % Similarity [9-10]. In evolution, another branch different from SARS-COV and MERS-COV is formed. The diameter of the SARS-COV-2 viral particles...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/569G01N33/68G01N33/543
CPCG01N33/56983G01N33/6854G01N33/54306G01N33/54393G01N2333/165G01N2469/20
Inventor 黄若磐方建民罗树红朱思为谢社火易玉华
Owner RAYBIOTECH INC GUANGZHOU