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Macrophage exosome membrane coated bionic nanoparticle as well as preparation method and application thereof

A technology of macrophages and nanoparticles, applied in the direction of pharmaceutical formulas, medical preparations with non-active ingredients, medical preparations containing active ingredients, etc., can solve the problem of fewer genes, etc., to promote gene silencing, effective treatment, reduce The effect of degree of infiltration

Active Publication Date: 2021-08-03
THE FIRST AFFILIATED HOSPITAL OF WANNAN MEDICAL COLLEGE YIJISHAN HOSPITAL OF WANNAN MEDICAL COLLEGE
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Problems solved by technology

However, with conventional transfection of genes, the expression of genes in exosomes is less, and recent studies have also demonstrated that siRNAs are prone to degradation when electroporation induces siRNAs into exosomes, suggesting the need to establish a more reliable protocol

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  • Macrophage exosome membrane coated bionic nanoparticle as well as preparation method and application thereof
  • Macrophage exosome membrane coated bionic nanoparticle as well as preparation method and application thereof
  • Macrophage exosome membrane coated bionic nanoparticle as well as preparation method and application thereof

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Embodiment Construction

[0032] The embodiments of the present invention will be further described below in conjunction with the accompanying drawings and examples.

[0033] In the present invention, we explored biomimetic nanoparticles (EM-PLGA@Dnmt3aos smart silencer ), which is provided by PLGA @Dnmt3aos smart silencer Core and exosome membrane composition. Use EM-PLGA @Dnmt3aos smart silencer Inhibition of M2 macrophage polarization in AA (allergic asthma) was very significant ( figure 1 ), the biomimetic drug efficiently accumulated in the lungs and promoted gene silencing while reducing the degree of inflammatory cell infiltration of the airways. Therefore, we believe that exosomal membrane-based PLGA NPs contribute to the transfer of therapeutic Dnmt3aos smart silencer Delivery to the airway directs its more effective treatment in a mouse model of AA.

[0034] 1. Materials and methods

[0035] 1.1 Mice

[0036] C57BL / 6(H-2d) female mice (6-8 weeks, 20-22 g) were purchased from Qinglo...

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Abstract

The invention belongs to the field of biological medicine, and relates to a macrophage exosome membrane coated bionic nanoparticle as well as a preparation method and application thereof, and the macrophage exosome membrane coated bionic nanoparticle is formed by an exosome membrane coated nanoparticle PLGA (at) Dnmt3aossmartsilencer; the exosome membrane is separated from M2 macrophages from bone marrow; and the nano-particle PLGA (at) Dnmt3aossmartsilencer is formed by co-emulsification of a polylactic acid-glycolic acid copolymer PLGA and Dnmt3aossmartsilencer. Compared with the prior art, the application has the advantages that the EM-PLGA-(at) Dnmt3aossmartsilencer is used for remarkably inhibiting polarization of M2 macrophages in the allergic asthma (AA), the bionic medicine is effectively accumulated in the lung and promotes gene silencing, and meanwhile, the infiltration degree of inflammatory cells to the airway is reduced. Therefore, the PLGA NP based on the M2 macrophage exosome membrane is beneficial to delivery of the therapeutic Dnmt3aossmartsilencer to the airway, and the therapeutic Dnmt3aossmartsilencer is guided to be more effective in treatment in an AA mouse model.

Description

technical field [0001] The invention belongs to the field of biomedicine, and relates to a macrophage exosome membrane-coated biomimetic nanoparticle and a preparation method and application thereof. Background technique [0002] Asthma affects 300 million people worldwide and is estimated to affect 400 million by 2025, with allergic asthma (AA) accounting for 90% of asthma cases in children and 50% of asthma cases in adults. Macrophages are the most abundant immune cells in the lung (approximately 70% of all immune cells), and they play an important role in airway inflammation induced by environmental allergens. Adoptive transfer of M2 macrophages can aggravate allergic airway inflammation in mice, suggesting that the polarization of M2 macrophages plays an important role in AA attacks. Therefore, developing new strategies targeting M2 macrophages is considered a promising approach for the treatment of AA. [0003] In our previous study, thousands of lncRNAs were differen...

Claims

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Application Information

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IPC IPC(8): A61K9/51A61K47/46A61K47/34A61K47/59A61K47/69A61K31/7088A61P11/06A61P37/08
CPCA61K9/5153A61K9/5176A61K9/5192A61K31/7088A61K47/59A61K47/6935A61P11/06A61P37/08
Inventor 吕坤裴纬亚张莺莺
Owner THE FIRST AFFILIATED HOSPITAL OF WANNAN MEDICAL COLLEGE YIJISHAN HOSPITAL OF WANNAN MEDICAL COLLEGE
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