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Method for evaluating soothing efficacy of cosmetic raw materials

A technology for cosmetics and raw materials, applied in the analysis of materials, material analysis by optical means, instruments, etc., can solve the problems of high-throughput screening that cannot be efficiently, quickly and in large quantities, subjective factors in evaluation results, and long test periods, etc. Achieve objective and reliable evaluation results, meet high-throughput screening, and achieve the effect of short test period

Active Publication Date: 2021-08-03
清保(广州)生物科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] To sum up, there are at least one of the following limitations in the existing cosmetic soothing efficacy evaluation methods: long test period, high cost, inability to efficiently and quickly carry out large-scale high-throughput screening, selection of evaluation indicators and subjectivity of processing Strong, with subjective factors in the evaluation results

Method used

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  • Method for evaluating soothing efficacy of cosmetic raw materials
  • Method for evaluating soothing efficacy of cosmetic raw materials
  • Method for evaluating soothing efficacy of cosmetic raw materials

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] (1) Set up a blank control group: take zebrafish embryos fertilized for 16 hours, put them into a 96-well plate, 5 embryos per well, set 3 replicate wells in each group, add 200 μL of H-Buffer solution to each well, and culture for 1 Hour;

[0034] (2) Set up the model control group: take zebrafish embryos fertilized for 16 hours, put them into a 96-well plate, 5 embryos per well, set 3 duplicate wells for each group, add 200 μL of dodecane to each well in the form of a solution Sodium sulfonate was added at a concentration of 200 μmol / L and incubated for 1 hour. The concentration of the added sodium dodecylsulfonate solution is above the minimum percutaneous stimulation concentration.

[0035] (3) Set the sample group to be tested: take zebrafish embryos fertilized for 16 hours, put them into a 96-well plate, 5 embryos per well, set 3 duplicate wells for each group, and add 200 μL of glycyrrhizic acid to each well in the form of a solution A mixture of dipotassium and ...

Embodiment 2

[0038] (1) Set up a blank control group: take zebrafish embryos fertilized for 24 hours, put them into a 96-well plate, 5 embryos per well, set 3 replicate wells for each group, add 200 μL of H-Buffer solution to each well, and culture for 1 Hour;

[0039] (2) Set up the model control group: take zebrafish embryos fertilized for 24 hours, put them into a 96-well plate, 5 embryos per well, set 3 duplicate wells for each group, and add 200 μL of dodecane to each well in the form of a solution Sodium sulfonate was added at a concentration of 200 μmol / L and incubated for 1 hour;

[0040] (3) Set the sample group to be tested: take zebrafish embryos fertilized for 24 hours, put them into a 96-well plate, 5 embryos per well, set 3 duplicate wells for each group, add 200 μL of snow to each well in the form of a solution The mixture of glucoside and sodium dodecylsulfonate was incubated for 1 hour. The concentration of sodium dodecylsulfonate was 200 μmol / L, and the concentration of...

Embodiment 3

[0043] (1) Set up a blank control group: take zebrafish embryos fertilized for 36 hours, put them into a 96-well plate, 5 embryos per well, set 3 replicate wells for each group, add 200 μL of H-Buffer solution to each well, and culture for 1 Hour;

[0044] (2) Set up the model control group: take zebrafish embryos fertilized for 36 hours and place them in a 96-well plate, with 5 embryos per well, and set 3 duplicate wells for each group, and add 200 μL of dodecane to each well in the form of a solution Sodium sulfonate was added at a concentration of 200 μmol / L and incubated for 1 hour;

[0045] (3) Set up the sample group to be tested: take the zebrafish embryos fertilized for 36 hours, put them into a 96-well plate, and set up 3 replicate wells for each group, and add 200 μL of proanthocyanidins and The mixture of sodium dodecylsulfonate was incubated for 1 hour. The concentration of sodium dodecylsulfonate was 200 μmol / L, and the concentration of proanthocyanidins was wit...

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Abstract

The invention relates to the technical field of efficacy evaluation of cosmetic raw materials, and particularly discloses a method for evaluating the soothing efficacy of cosmetic raw materials. The method comprises the following steps: setting a blank control group: taking a zebra fish embryo as a tested object, and adding a non-irritant culture solution; setting a model control group: taking the zebra fish embryo as a tested object, and adding the cosmetic raw materials for inducing skin stimulation in a solution manner; setting a to-be-tested sample group: taking the zebra fish embryo as a tested object, and simultaneously adding a cosmetic raw material for inducing skin stimulation and a to-be-tested cosmetic raw material in a solution manner; and after the groups are cultured for the same time, observing and counting the times of zebrafish embryo spasm rolling in the blank control group, the model control group and the to-be-detected sample group. Compared with an existing method for evaluating the soothing effect of cosmetic raw materials, the method is short in test period, low in cost, capable of meeting high-throughput screening of the cosmetic raw materials, simple in experiment operation and objective and reliable in evaluation result.

Description

technical field [0001] The invention relates to the technical field of efficacy evaluation of cosmetic raw materials, in particular to a method for evaluating the soothing efficacy of cosmetic raw materials. Background technique [0002] At present, there are some cosmetics on the market that claim to contain raw materials that have a soothing effect on the skin, and the evaluation methods for the soothing effect of cosmetics include: physical and chemical methods, biochemical methods, cell biology methods, three-dimensional reconstruction skin model replacement methods, and animal experiments. and human experiments. [0003] (1) Physicochemical method is a convenient method to evaluate the effect of cosmetics by testing their physical properties and the content of specific chemical components. At present, the evaluation of soothing efficacy of cosmetics is mostly qualitative and quantitative testing of specific functional components. Ceramide is an important component of h...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/00G01N21/84
CPCG01N33/00G01N21/84
Inventor 赵海山何健华郭清泉王雅馨何露露罗小雨张艺瀚
Owner 清保(广州)生物科技有限公司
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