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Method for carrying out colored hair dyeing based on enzymatic oxidation of tyrosine derivatives

A technology of tyrosine and derivatives, applied in the field of chemistry, can solve problems such as unpleasant olfactory experience, inability to achieve other color dyeing, difficulty, etc., and achieve the effects of convenient and fast dyeing process, good biological adaptability, and easy operation.

Active Publication Date: 2021-08-13
天津北洋康肽生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the oxidation process of the selected PDA requires the addition of ammonia, H 2 o 2 and metal ions, which cannot meet the requirements of green chemistry and practical applications, and will also bring unpleasant olfactory experience during the coloring process, which is still difficult to promote and use in practice
At the same time, the colors that can be produced in the existing reports are all darker colors such as black, and it is still impossible to dye other colors, such as yellow, red, purple, etc. The preparation of rich colors with a full spectrum has always been the long-term goal of people.

Method used

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  • Method for carrying out colored hair dyeing based on enzymatic oxidation of tyrosine derivatives
  • Method for carrying out colored hair dyeing based on enzymatic oxidation of tyrosine derivatives
  • Method for carrying out colored hair dyeing based on enzymatic oxidation of tyrosine derivatives

Examples

Experimental program
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Effect test

Embodiment 1

[0043] A method for coloring hair based on enzymatic oxidation of tyrosine derivatives, comprising the steps of:

[0044] (1) washing human hair with water (the hair is white);

[0045] (2) dissolving the tyrosine derivative in PBS buffer solution with pH=7 to obtain a tyrosine derivative-PBS buffer solution with a concentration of 10 mg / mL;

[0046] (3) dissolving polyphenol oxidase in PBS buffer solution with pH=7 to obtain polyphenol oxidase-PBS buffer solution;

[0047] (4) Soak the hair after washing with water in the solution obtained in step (2); under stirring, add the polyphenol oxidase-PBS buffer solution obtained in step (3), so that the final concentration of polyphenol oxidase is 10U / mL, react at 37°C for 3 hours;

[0048] (5) Rinse with water, blot dry the surface moisture of the hair, and bake at 50°C for 8 minutes to fix the color. The resulting hair color is reddish brown, see figure 1 Sample No. 2.

[0049] Tyrosine derivatives are: N-tert-butoxycarbonyl...

Embodiment 2

[0053] A method for coloring hair based on enzymatic oxidation of tyrosine derivatives, comprising the steps of:

[0054] (1) washing human hair with water (the hair is white);

[0055] (2) Dissolving the tyrosine derivative in PBS buffer solution with pH=6.5 to obtain a tyrosine derivative-PBS buffer solution with a concentration of 1 mg / mL;

[0056] (3) dissolving polyphenol oxidase in PBS buffer solution with pH=6.5 to obtain polyphenol oxidase-PBS buffer solution;

[0057] (4) Soak the hair after washing with water in the solution obtained in step (2); under shaking, add the polyphenol oxidase-PBS buffer solution obtained in step (3), so that the final concentration of polyphenol oxidase is 8U / mL, react at 40°C for 3 hours;

[0058] (5) Rinse with water, blot dry the surface moisture of the hair, and dry at 40°C for 10 minutes to fix the color. The resulting hair color is light pink, see figure 1 Sample No. 3.

[0059] The tyrosine derivative is: 9-fluorenylmethoxycar...

Embodiment 3

[0063] A method for coloring hair based on enzymatic oxidation of tyrosine derivatives, comprising the steps of:

[0064] (1) washing human hair with water (the hair is white);

[0065] (2) Dissolving the tyrosine derivative in PBS buffer solution with pH=7 to obtain a tyrosine derivative-PBS buffer solution with a concentration of 20 mg / mL;

[0066] (3) dissolving polyphenol oxidase in PBS buffer solution with pH=7 to obtain polyphenol oxidase-PBS buffer solution;

[0067] (4) Soak the hair after washing with water in the solution obtained in step (2); under stirring, add the polyphenol oxidase-PBS buffer solution obtained in step (3), so that the final concentration of polyphenol oxidase is 12U / mL, react at 25°C for 2 hours;

[0068] (5) Rinse with water, blot dry the surface moisture of the hair, and bake at 60°C for 5 minutes to fix the color. The resulting hair color is auburn, see figure 1 Sample No. 4.

[0069] The tyrosine derivatives are: benzyloxycarbonyl-L-tyro...

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Abstract

The invention discloses a method for carrying out colored hair dyeing based on enzymatic oxidation of tyrosine derivatives. The method comprises the following steps: (1) washing human hair with water; (2) dissolving the tyrosine derivatives in PBS (Phosphate Buffer Solution) to obtain tyrosine derivative-PBS solution; (3) dissolving polyphenol oxidase in the PBS to obtain polyphenol oxidase-PBS solution; (4) soaking the hair washed with water in the solution obtained in the step (2), adding the polyphenol oxidase-PBS solution obtained in the step (3) under the shaking or stirring condition , and performing a reaction; and (5) washing with water, absorbing moisture on the surface of the hair, and carrying out drying and color fixing. The method disclosed by the invention is simple and convenient to operate, green, friendly, safe, non-toxic, mild in reaction and stable. The method is carried out under the chemical enzymatic reaction, alkali and additional additives are not used, a dye cannot permeate into the hair cortex, and the method is a green dyeing technology which does not damage the hair.

Description

technical field [0001] The invention belongs to the technical field of chemistry, and in particular relates to a method for coloring hair based on enzymatic oxidation of tyrosine derivatives. Background technique [0002] As the saying goes, everyone has the love of beauty. Rich and changeable hair color has long been the standard for trendy people, and with age, the fading and changing of hair color is also a problem for many people. Since ancient times, hair coloring has been the oldest among people. One of the most popular grooming practices reflects a long-standing dissatisfaction with natural hair color. Since Hofmann observed 150 years ago that p-phenylenediamine produces shades of brown on a variety of substrates when exposed to oxidizing agents, including air, it has been the basis for the most commonly used hair coloring process, although chemical The field is constantly advancing and developing, but chemical hair dye fixation is still the most commonly used hair...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K8/44A61K8/66A61Q5/10A61Q17/04
CPCA61K8/44A61K8/66A61Q5/10A61Q17/04
Inventor 王跃飞齐崴申雨禾苏荣欣
Owner 天津北洋康肽生物科技有限公司
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