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Application of wheat germ agglutinin to preparation of product for inhibiting coronavirus

A technology of wheat germ agglutinin and coronavirus, applied in antiviral agents, medical preparations containing active ingredients, and resistance to vector-borne diseases, etc., can solve the problems that have not yet been applied to wheat germ agglutinin, and achieve good application value Effect

Active Publication Date: 2021-08-13
NAT INST FOR FOOD & DRUG CONTROL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no application of wheat germ agglutinin in the treatment of infections caused by coronaviruses SARS-CoV, MERS-CoV, SARS-CoV-2 and SARS-CoV-2 mutants

Method used

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  • Application of wheat germ agglutinin to preparation of product for inhibiting coronavirus
  • Application of wheat germ agglutinin to preparation of product for inhibiting coronavirus
  • Application of wheat germ agglutinin to preparation of product for inhibiting coronavirus

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0082] Example 1 Detection of plant agglutin on SARS-COV-2 s protein binding activity and antiviral activity

[0083] 1, experimental materials

[0084] Lentil lectin, maternal germ (WGA), non-infarcting hydrocogene (SSL), peanut lectin, Huiguogeli (MAL) is purchased from Wako (Japan) ; Mandalage group (DSL), succinoyl-accompanied kidnoamin A (Succ-con a), Snowdotin (GNL), plant aggregate (PHA-E), leukocytin (PHA-L) ), Plants from red kidney beans - P (PHA-M) and plant aggregate - P (PHA-P) are purchased from Sigma Aldrich; HEK293 cell expressed SARS-COV-2 S trimer At Acro Biosystems (China, Beijing); 293T cells are provided by the American Typical Culture Preservation Center (ATCC); HuH7 cells are provided by the Japanese Research Basic Resources Savings Center (JCRB); buhhan-hu-2spike 1, Mn908947; HCOV-19 / South Africa / Krisp-K007869 / 2020, B.1.351, Epi_ISL_860630; HCOV-19 / ENGLAND / QEUH-F56F0F / 2021, B.1.1.7, EPI_ISL_852526; HCOV-19 / Brazil / AM -991 / 2020, P.1, Epi_ISL...

Embodiment 2

[0097] Example 2 Inhibitory effect of plant agglutin on SARS-COV-2 s protein mutant and SARS-COV-2 epidemic mutant infection

[0098] In this example, various plant aggregates are detected in inhibitory effects of SARS-COV-2 S protein mutant and SARS-COV-2 epidemic mutant infection, including British epidemic strains B.1.1.7. , South African epidemic mutant strain B.1.351, Brazilian epidemic mutant strain P.1, combined mutant strain D614G + I472V, combined mutant strain D614G + A435S, A222V, K417N, S477N, E484K, N501Y, combined mutant A222V + D614G, combined mutation The strain K417N + D614G, combined mutant strain S477N + D614G, combined mutant strain E484K + D614G, combined mutant strain N501Y + D614G and other mutants;

[0099] The agglutin is mainly directed to the glycosyl portion of the glycoprotein. In the present embodiment, the inventors have studied whether the changes in the saccharification site of the SARS-COV-2 s protein will affect the neutralization of the concentr...

Embodiment 3

[0109] Example 3 Detection of blood cell agglutivity and cytotoxicity of plant lectin

[0110] Since many natural lectins cause blood cell agglutination, in the present embodiment, the inventors have studied blood cell agglutination activity of different plant aggregates, and the cytotoxicity of HUH7 and 293T cells was studied.

[0111] 1. Detection of blood cell agglutination activity of plant lectin

[0112] The blood cell agglutination activity of plant lectin was detected by a chicken blood specimen, and the cock red blood cells were washed with PBS, and 50 μl of 2 times series diluted plant agglutinum solution and an equivalent of red blood cells were resuspended. The solution was mixed in a 96-well round bottom plate, and the flu antigen (B / Maryland / 15 / 2016, NIBSC-UK-EN63QG, NIBSC code: 18 / 104, HA: 69μg / ml) was diluted with a positive control. The PBS solution was used as a negative control and incubated the plate for 1 hour under room temperature, and the blood cell a...

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Abstract

The invention discloses application of wheat germ agglutinin to preparation of a product for inhibiting a coronavirus, and it is first time for the invention to finds that the wheat germ agglutinin has relatively high antiviral activity on SARS-CoV, MERS-CoV, SARS-CoV-2 and SARS-CoV-2 mutant strains, and also shows certain antiviral activity on vesicular stomatitis virus (VSV), can be used for treating and preventing infection caused by the coronavirus, can be used as a candidate medicine for preventing and treating a coronavirus infection, and has relatively high application value.

Description

Technical field [0001] The present invention belongs to the field of biopharmaceutical technology, in particular, involving the use of wheat germ gerotimin in the preparation of products inhibiting coronaviruses. Background technique [0002] Coronaviruses, Covs is one of the major viral pathogens that lead to respiratory infections (fehr ar, perlman s.coronaviruses: an overview of their replication andpathogenesis [j] .coronaviruses, 2015: 1-23.), Coronavirus belongs to Nested virus, coronavirus, coronavirus genomic and subgenuys contain at least 6 open reading frames (ORF), generally 5 'cap (Leader) and 3' end (ENDS) sequence, 5 ' Hat open reading box codes produce multiple non-structural proteins, non-structural proteins involved in virus transcription and replication; coronaviruses have at least 4 main structural proteins, including spikeprotein, s protein, envelope protein (Envelope Protein, E protein), membrane protein (M protein) and Nucleocapsid Protein, N protein), these...

Claims

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Application Information

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IPC IPC(8): A61K38/16A61P31/14A61P31/12A61P31/22
CPCA61K38/168A61P31/14A61P31/12A61P31/22Y02A50/30
Inventor 王佑春王兰黄维金王文波李倩倩吴佳静武刚于传飞郭璐韵郭莎
Owner NAT INST FOR FOOD & DRUG CONTROL
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