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Stem cell exosome freeze-dried powder-based kit and application

An exosome and stem cell technology, applied in artificial cell constructs, cell culture active agents, animal cells, etc., can solve the problems of undisclosed cell culture effects and no stem cell extracts.

Pending Publication Date: 2021-08-13
博品(上海)生物医药科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this prior art does not involve the combination of stem cell extract and conventional medium, nor does it disclose the influence of the combined culture system on cell culture

Method used

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  • Stem cell exosome freeze-dried powder-based kit and application
  • Stem cell exosome freeze-dried powder-based kit and application
  • Stem cell exosome freeze-dried powder-based kit and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] A kit based on stem cell exosome freeze-dried powder, consisting of DMEM / F12 medium (Gibco), human serum albumin (Jet Behring), stem cell exosome freeze-dried powder, and ultrapure water, wherein, The volume of human albumin is 5% of the volume of DMEM / F12 medium, and the dosages of stem cell exosome freeze-dried powder, DMEM / F12 medium, and ultrapure water are 5 mg, 100 mL, and 5 mL, respectively.

Embodiment 2

[0047] A kit based on stem cell exosome freeze-dried powder, consisting of DMEM / F12 medium (Gibco), human serum albumin (Jet Behring), stem cell exosome freeze-dried powder, and ultrapure water, wherein, The volume of human albumin is 4% of the volume of DMEM / F12 medium, and the dosages of stem cell exosome freeze-dried powder, DMEM / F12 medium, and ultrapure water are 5 mg, 100 mL, and 5 mL, respectively. According to the experimental method of the application example, the cells grow to the 7th day, and the cells can be subcultured.

Embodiment 3

[0049] A kit based on stem cell exosome freeze-dried powder, consisting of DMEM / F12 medium (Gibco), human serum albumin (Jet Behring), stem cell exosome freeze-dried powder, and ultrapure water, wherein, The volume of human albumin is 6% of the volume of DMEM / F12 medium, and the dosages of stem cell exosome freeze-dried powder, DMEM / F12 medium, and ultrapure water are 5 mg, 100 mL, and 5 mL, respectively. According to the experimental method of the application example, the cells grow to the 7th day, and the cells can be subcultured.

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PUM

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Abstract

The invention discloses a stem cell exosome freeze-dried powder-based kit and application. The kit comprises a DMEM / F12 culture medium, human serum albumin and stem cell exosome freeze-dried powder. According to the invention, the stem cell exosome freeze-dried powder is obtained by drying a stem cell extracting solution; stem cells are induced by normal saline to prepare the extracting solution; the extracting solution are diversified in ingredient and contain abundant various stem cell exosome cytokines, extracellular matrixes, exosome vesicles and the like; and an unexpected technical effect can be achieved by culturing endometrial cells through composite components.

Description

technical field [0001] The invention belongs to biotechnology, and in particular relates to a kit and application based on stem cell exosome freeze-dried powder. Background technique [0002] Studies suggest that the decrease in the number or function of endometrial cells will lead to thin endometrium (endometrial thickness <7mm) or endometrial dysfunction, which will affect embryo implantation (Schwab KE, Gargett CE. Co-expression of two perivascular cell markers isolates mesenchymal stem-like cells from human endometrium). The etiology of endometrial injury includes postpartum curettage, spontaneous abortion, induced abortion, endometrial ablation, pelvic tuberculosis, infection, etc., resulting in destruction of the endometrial basal layer and myometrium. When the basal layer of the endometrium is damaged, it may result in loss or damage of endometrial cells. In the prior art, the primary stem cells are inoculated in the culture medium, the primary culture is perform...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/071
CPCC12N5/0682C12N2500/84C12N2501/998
Inventor 董健伸徐汝强孙永沛程洪斌王晓东孟凡江
Owner 博品(上海)生物医药科技有限公司
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