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Low-molecular-weight polysaccharide with antithrombotic activity

A technology with antithrombotic activity and low molecular weight, applied in the field of low molecular weight polysaccharides, can solve problems such as low bioavailability, high viscosity, and weakened biological activity, and achieve the effects of improving antithrombotic activity, increasing degradation rate, and increasing extraction rate

Inactive Publication Date: 2021-08-20
杭州鹿扬科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Generally, the extracted fucoidan sulfate polysaccharides have relatively large molecular weight, high viscosity, poor solubility, poor absorption, and low bioavailability, so the polysaccharides are degraded into small molecular weight polysaccharides.
It is generally believed that polysaccharides with smaller molecular weights are easier to reach the active site through tissue cells, and their effective parts are more likely to combine with the reacting ligands, thereby making them more active. However, if the molecular weight is too small, its biological activity may be reduced. weakened

Method used

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  • Low-molecular-weight polysaccharide with antithrombotic activity
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  • Low-molecular-weight polysaccharide with antithrombotic activity

Examples

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Effect test

Embodiment 1

[0038] A preparation method of antithrombotic low molecular weight sargassum polysaccharide,

[0039] (1) The operating conditions for pretreatment are: wash Sargassum with water, dry at 50° C., add acetone after crushing, soak at 25° C. for 2 hours, remove acetone to decolorize and degrease to obtain Sargassum powder.

[0040] (2) The operating conditions of the purification process are as follows: add Sargassum powder to hydrochloric acid solution, add additives sodium sulfonate and ammonium acetate, extract at a pressure of 100kPa and a temperature of 50°C for 3 hours, adjust the pH to 6.5 after the extraction, diatoms The soil is filtered and concentrated, and calcium chloride is added to degumming to obtain the crude liquid of sargassum polysaccharide; the solid-liquid ratio of sargassum powder and hydrochloric acid solution is 1:10 (g / mL), the concentration of hydrochloric acid solution is 0.05mol / L, sulfonic acid The added amount of sodium is 0.3wt% of the hydrochloric ...

Embodiment 2

[0044] A preparation method of antithrombotic low molecular weight sargassum polysaccharide,

[0045] (1) The operating conditions for pretreatment are: wash Sargassum with water, dry at 50° C., add acetone after crushing, soak at 25° C. for 2 hours, remove acetone to decolorize and degrease to obtain Sargassum powder.

[0046] (2) The operating conditions of the purification process are as follows: add Sargassum powder to hydrochloric acid solution, add additives sodium sulfonate and ammonium acetate, extract at a pressure of 100kPa and a temperature of 50°C for 3 hours, adjust the pH to 6.5 after the extraction, diatoms The soil is filtered and concentrated, and calcium chloride is added to degumming to obtain the crude liquid of sargassum polysaccharide; the solid-liquid ratio of sargassum powder and hydrochloric acid solution is 1:10 (g / mL), the concentration of hydrochloric acid solution is 0.05mol / L, sulfonic acid The addition amount of sodium is 3wt% of hydrochloric aci...

Embodiment 3

[0050] A preparation method of antithrombotic low molecular weight sargassum polysaccharide,

[0051] (1) The operating conditions for pretreatment are: wash Sargassum with water, dry at 50° C., add acetone after crushing, soak at 25° C. for 2 hours, remove acetone to decolorize and degrease to obtain Sargassum powder.

[0052] (2) The operating conditions of the purification process are as follows: add Sargassum powder to hydrochloric acid solution, add additives sodium sulfonate and ammonium acetate, extract at a pressure of 100kPa and a temperature of 50°C for 3 hours, adjust the pH to 6.5 after the extraction, diatoms The soil is filtered and concentrated, and calcium chloride is added to degumming to obtain the crude liquid of sargassum polysaccharide; the solid-liquid ratio of sargassum powder and hydrochloric acid solution is 1:10 (g / mL), the concentration of hydrochloric acid solution is 0.05mol / L, sulfonic acid The addition amount of sodium is 3wt% of hydrochloric aci...

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Abstract

The invention discloses a low-molecular-weight polysaccharide with antithrombotic activity. The low-molecular-weight polysaccharide is prepared by the following steps of: pretreating gulfweed to obtain pretreated gulfweed powder; extracting the pretreated gulfweed powder to obtain a gulfweed polysaccharide crude solution; purifying the gulfweed polysaccharide crude solution to obtain purified gulfweed polysaccharide; and degrading the purified gulfweed polysaccharide to obtain the small-molecular-weight gulfweed polysaccharide. In the extraction process, an extraction aid sodium sulfonate and ammonium acetate are added to assist a hydrochloric acid solution to extract the gulfweed polysaccharide. According to the low-molecular-weight polysaccharide with the antithrombotic activity, the extraction rate of the gulfweed polysaccharide is increased, the retaining amount of sulfate radicals is increased, the antithrombotic activity is improved, the gulfweed polysaccharide has small molecular weight, and the degradation effect is improved.

Description

[0001] This application is a divisional application of Chinese patent application 2020104223979 submitted on May 19, 2020. technical field [0002] The invention belongs to the field of biomedicine, and in particular relates to a low-molecular-weight polysaccharide with antithrombotic activity. Background technique [0003] Sargassum resources are very rich, widely distributed in warm water and warm waters, such as the Sea of ​​Okhotsk, Thousand Islands, Japan and North Korea abroad, as well as Hong Kong, Shantou, Xuwen, Zhanjiang and other places in China. There are many kinds of Sargassum, and hundreds of them have been named in the world. There are about 100 kinds of Sargassum along the coast of mainland my country, and more than 50 kinds have been named. Sargassum is a perennial algae that mostly grows on rocky marshes or rocks in low tidal zones. It has a large yield and is easy to obtain. The active polysaccharide rich in brown algae, fucoidan sulfate is a water-solub...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00A61K31/715A61P7/02
CPCA61K31/715A61P7/02C08B37/0003
Inventor 不公告发明人
Owner 杭州鹿扬科技有限公司
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