Novel coronavirus COVID-19 fluorescent quantitative PCR detection primer and probe, kit and detection method

A technology for COVID-19, detection kits, applied in biochemical equipment and methods, microbial determination/inspection, DNA/RNA fragments, etc., to achieve the effect of good quantitative linear range, reduced risk of contamination, and reduced risk of contamination

Pending Publication Date: 2021-08-24
ACAD OF MILITARY SCI PLA CHINA ACAD OF MILITARY MEDICAL SCI INST OF MILITARY VETERINARY MEDICINE
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Problems solved by technology

At present, there is no new coronavirus COVID-19 nucleic acid detection kit for detecting experimental mice, so it is necessary to develop a new coronavirus...

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  • Novel coronavirus COVID-19 fluorescent quantitative PCR detection primer and probe, kit and detection method
  • Novel coronavirus COVID-19 fluorescent quantitative PCR detection primer and probe, kit and detection method
  • Novel coronavirus COVID-19 fluorescent quantitative PCR detection primer and probe, kit and detection method

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Embodiment 1

[0044] A novel coronavirus COVID-19 fluorescent quantitative PCR detection kit for mouse experiments, the main components of the kit are shown in Table 1.

[0045] Table 1

[0046]

[0047] The primer pair set in Table 1 is composed of the first primer pair and the internal standard primer pair, the first primer pair is the detection primer designed for the N gene of the new coronavirus COVID-19, including the forward sequence shown in SEQ ID NO.1 Primer and the reverse primer shown in SEQ ID NO.2, the internal standard primer pair is the primer designed for the mouse internal reference gene Beta actin, including the forward primer shown in SEQ ID NO.4 and the primer shown in SEQ ID NO.5 Reverse primers indicated. The probe set is composed of the first probe SEQ ID NO.3 and the internal control probe SEQ ID NO.6, the first probe is a probe designed for the amplified sequence of the first primer pair, and the internal control probe It is a probe designed for the amplified ...

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Abstract

The invention relates to a novel coronavirus COVID-19 fluorescent quantitative PCR (Polymerase Chain Reaction) detection primer, a probe, a kit and a detection method, and the novel coronavirus COVID-19 fluorescent quantitative PCR detection primer and probe comprise specific primers aiming at a novel coronavirus COVID-19 N gene as shown in SEQ ID NO.1-2, and a first probe as shown in SEQ ID NO.3. The novel coronavirus COVID-19 detection kit provided by the invention can rapidly, accurately and sensitively detect the novel coronavirus COVID-19 in mouse tissues, the detection sensitivity is as high as 10 copies/mL, quantitative analysis can be carried out while qualitative analysis is carried out on viruses in the mouse tissues, and the quantitative linear range is good. The movel coronavirus COVID-19 fluorescent quantitative PCR detection primer and probe, the kit and the detection methodare used for detecting the novel coronavirus COVID-19 in laboratory mouse tissues, and have a relatively high application value.

Description

technical field [0001] The invention belongs to the technical field of determination or detection methods including enzymes, nucleic acids or microorganisms, and in particular relates to a novel coronavirus COVID-19 fluorescent quantitative PCR detection primer, probe, kit and detection method for mouse experiments. Background technique [0002] The novel coronavirus COVID-19 can cause respiratory symptoms, fever, cough, shortness of breath, and dyspnea in humans. In more severe cases, infection can lead to pneumonia, severe acute respiratory syndrome, kidney failure, and even death. The novel coronavirus COVID-19 is raging around the world, seriously endangering human health. [0003] At present, the world is stepping up the research and development of vaccines and drugs against the new coronavirus COVID-19. Both vaccine preparation and drug development are inseparable from animal models. Mouse models play an extremely important role in the research of infectious diseases. ...

Claims

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Application Information

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IPC IPC(8): C12Q1/70C12Q1/686C12N15/11
CPCC12Q1/701C12Q1/686C12Q2563/107C12Q2545/114C12Q2521/107Y02A50/30
Inventor 赵宗正刘丽娜郭振东张春茂高玉伟
Owner ACAD OF MILITARY SCI PLA CHINA ACAD OF MILITARY MEDICAL SCI INST OF MILITARY VETERINARY MEDICINE
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