Novel coronavirus COVID-19 fluorescent quantitative PCR detection primer and probe, kit and detection method
A technology for COVID-19, detection kits, applied in biochemical equipment and methods, microbial determination/inspection, DNA/RNA fragments, etc., to achieve the effect of good quantitative linear range, reduced risk of contamination, and reduced risk of contamination
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[0044] A novel coronavirus COVID-19 fluorescent quantitative PCR detection kit for mouse experiments, the main components of the kit are shown in Table 1.
[0045] Table 1
[0046]
[0047] The primer pair set in Table 1 is composed of the first primer pair and the internal standard primer pair, the first primer pair is the detection primer designed for the N gene of the new coronavirus COVID-19, including the forward sequence shown in SEQ ID NO.1 Primer and the reverse primer shown in SEQ ID NO.2, the internal standard primer pair is the primer designed for the mouse internal reference gene Beta actin, including the forward primer shown in SEQ ID NO.4 and the primer shown in SEQ ID NO.5 Reverse primers indicated. The probe set is composed of the first probe SEQ ID NO.3 and the internal control probe SEQ ID NO.6, the first probe is a probe designed for the amplified sequence of the first primer pair, and the internal control probe It is a probe designed for the amplified ...
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