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Method for determining enrichment rate of organochlorine pesticides of human body

A technology of organochlorine and enrichment rate, applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problem that the intake of pesticides cannot accurately represent or correspond to the accumulation of pesticides or the accumulation of pesticides in the body, and it is difficult to ensure that the concentration of representative pesticides is consistent Problems such as stability and data stability, cumulative error of calculation results, etc., to achieve the effect of small measurement error, improved solvent system, and fewer experimental steps

Pending Publication Date: 2021-08-24
NAT RESERACH CENT OF GEOANALYSIS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The shortcomings of this type of method are: 1. It is necessary to investigate the concentration of OCPs in a variety of foods and air, and the workload is large, and because this is an indirect calculation method, resulting in cumulative errors in calculation results; 2. It is difficult to ensure The representativeness of the samples taken orally and the consistency of the pesticide concentration present in it and the stability of the data; 3. The pesticide intake cannot accurately represent or correspond to the accumulation or enrichment of the pesticide in the body, which is likely to further cause large error

Method used

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  • Method for determining enrichment rate of organochlorine pesticides of human body
  • Method for determining enrichment rate of organochlorine pesticides of human body
  • Method for determining enrichment rate of organochlorine pesticides of human body

Examples

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Effect test

Embodiment 1

[0024] Breast milk samples were collected from lactating women during two consecutive lactation periods. Collect breast milk samples within 30 days after the start of each lactation period and within 30 days before the end of each lactation period. A total of four breast milk samples were collected, about 20 mL were collected in brown glass bottles, sealed, and stored in a freezer at -20°C. Before sample analysis, take the sample out of the refrigerator, return to room temperature, and shake well for later use.

[0025] Accurately pipette 10 mL of the above four samples respectively, record the accurate mass m of each sample, and transfer the sample to a 100 mL separating funnel without damage. Add U.S.EPA 8081 / 8270 substitute 2,4,5,6-tetrachloro-m-xylene and dibutyl chlorendate mixture (40μL×1.0μg / mL); shake thoroughly with 5mL ammonia water and 10mL ethanol Mix well, 15mL of diethyl ether is fully shaken for mixing, 25mL of n-hexane is fully shaken for 100 times, and then t...

Embodiment 2

[0028]Breast milk samples were collected from lactating women during two consecutive lactation periods. Collect breast milk samples within 30 days after the start of each lactation period and within 30 days before the end of each lactation period. A total of four breast milk samples were collected, about 20 mL were collected in brown glass bottles, sealed, and stored in a freezer at -20°C. Before sample analysis, take the sample out of the refrigerator, return to room temperature, and shake well for later use.

[0029] Accurately pipette 10 mL of the above four samples respectively, record the accurate mass m of each sample, and transfer the sample to a 100 mL separating funnel without damage. Add the U.S.EPA 8081 / 8270 substitute 2,4,5,6-tetrachloro-m-xylene and dibutyl chlorhexenate in sequence (40μL×1.0μg / mL); 50mL of n-hexane shake well to extract 100 After the second time, let it stand for layering. The upper organic phase was collected and the aqueous breast milk sample...

Embodiment 3

[0032] Breast milk samples were collected from lactating women during two consecutive lactation periods. Collect breast milk samples within 30 days after the start of each lactation period and within 30 days before the end of each lactation period. A total of four breast milk samples were collected, about 20 mL were collected in brown glass bottles, sealed, and stored in a freezer at -20°C. Before sample analysis, take the sample out of the refrigerator, return to room temperature, and shake well for later use.

[0033] Accurately pipette 10 mL of the above four samples respectively, record the accurate mass m of each sample, and transfer the sample to a 100 mL separating funnel without damage. Add the U.S.EPA 8081 / 8270 substitute 2,4,5,6-tetrachloro-m-xylene and the mixed standard of dibutyl chlorendate (40μL×1.0μg / mL); 75mL n-hexane / acetone (2: 1, volume ratio) After fully shaking and extracting for 100 times, let it stand for stratification. Collect the upper organic phas...

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Abstract

The invention provides a method for determining the annual enrichment rate of organochlorine pesticides (OCPs) of a human body, and belongs to the field of analysis technologies and evaluation methods. The invention aims to calculate and evaluate the enrichment speed of the human body OCPs in a certain period of time so as to provide more comprehensive, more direct and more accurate evaluation data for the exposure level and toxicity risk evaluation of the human body OCPs. The method comprises the following steps: collecting breast milk samples at the end and start of two continuous lactation periods of a female in the lactation period, analyzing the concentration and difference value of the OCPs in unit fat of the breast milk, calculating the enrichment speed of the human body OCPs every year between the two lactation periods, and improving the extraction rate of measured components in the samples through a specific solvent system. Therefore, the measurement accuracy is improved and the measurement error is reduced. The method can be used for different individuals and can also be used for evaluating the enrichment speed, the accumulation level, the exposure risk and the trend change of the overall OCPs of regional crowds.

Description

technical field [0001] The invention relates to a method for measuring the enrichment rate of human body organochlorine pesticides (organic chlorine pesticides, OCPs), preferably the annual enrichment rate, belonging to the field of pesticide detection and analysis. Background technique [0002] Organochlorine pesticides are organic compounds containing chlorine in the components used to prevent and control plant diseases and insect pests. It plays an important role in crop yield increase and disease control, but its biological toxicity and difficult to degrade characteristics make it a serious environmental pollutant. Human beings are in an environment where organochlorine pesticides are used, and it is inevitable that they will ingest organochlorine pesticides through various channels. They are further enriched in the body, thereby affecting human health. [0003] Methods exist in the prior art for measuring the daily intake or intake rate of OCPs in humans. Current res...

Claims

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Application Information

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IPC IPC(8): G01N30/88
CPCG01N30/88G01N2030/884
Inventor 宋淑玲佟玲潘萌
Owner NAT RESERACH CENT OF GEOANALYSIS
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