Anti-tumor pharmaceutical composition, and preparation method and application thereof
A composition and anti-tumor technology, applied in the field of anti-tumor drugs, can solve the problems of methotrexate and pimozide tumors that have not yet been seen, and achieve synergistic anti-tumor tumor metastasis, prevent tumor metastasis, and good safety Effect
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Embodiment 1
[0026] Example 1, Pimozide and MTX combined to inhibit MKN45 cells
[0027] 1. Method
[0028] MKN45 cell line (human gastric cancer cell line) was cultured in vitro, the medium was RPMI 1640 complete medium (RPMI1640 basal medium + 10% newborn bovine serum), and the cells were placed at 37°C, 5% CO 2 cultured in a cell culture incubator. After the cell confluency reached 80%, the adherent cells were digested with 0.25% trypsin. After the cells were digested, RPMI 1640 complete medium was added to stop the digestion. After centrifugation at 300g×5min, the cells were collected. Resuspend the cell pellet in complete medium to make a single cell suspension, and perform cell counting. Cells were diluted with complete medium and seeded in a 96-well plate at a density of 12,000 cells per well, with a volume of 100 μL per well. Set blank control wells: only contain 100 μL complete medium; cell control wells: only contain cells and complete medium, without drug treatment; drug tr...
Embodiment 2
[0041] Example 2, Pimozide combined with MTX inhibits MKN74 cells
[0042] 1. Method
[0043] Taking the MKN74 cell line (human gastric cancer highly metastatic cell line) as the test object, it was treated by the method in Example 1 (the cells were cultured in a 96-well plate at a density of 8000 / well, and the medium was also RPMI 1640 complete culture medium).
[0044] 2. Results
[0045]The results of using Pimozide (pimozide) or MTX (methotrexate) alone are shown in Table 3. When Pimozide is used alone, the dose of Pimozide is too small (1μM) has almost no inhibitory effect on MKN74 cells, only large doses of Pimozide (2 ~ 4μM) can inhibit MKN74 cells (survival rate <70%), with the increase of Pimozide concentration , the inhibitory effect increases. When MTX was used alone, the inhibitory effect on MKN74 cells was poor.
[0046] The results of the combined use of Pimozide (pimozide) and MTX (methotrexate) are shown in Table 3. It can be seen from Table 3 that the co...
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