Application of saikosaponin in resisting colon cancer
A saikosaponin, colon cancer technology, applied in the field of medicine, can solve the problems of unacceptable, long radiation therapy cycle and high cost for elderly patients
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[0091] Preparation of SDS-PAGE gel: Take a dry and clean glass plate, assemble the gel frame, add various ingredients according to the following table to prepare the lower separation gel:
[0092] Table 2 Prepare different volumes of separation gel components
[0093] Table 2 preparation of separate gel composition tables of different volumes
[0094]
[0095]
[0096] Wait for 30 minutes, after the separation gel is completely solidified, prepare the upper stacking gel according to the following table:
[0097] Table 3 prepares different volumes of stacking gel components
[0098] Table 3 preparation of concentrated gum composition tables of different volumes
[0099]
[0100] After adding the corresponding ingredients, insert the 10-hole comb quickly (no air bubbles can be generated), and wait for the upper layer of concentrated gel to solidify before proceeding to the next step.
[0101] ② Electrophoresis: add protein samples and markers into the sample wells ac...
Embodiment 1
[0124] Example 1 Detection of cell proliferation by CCK-8 method
[0125] Treat SW480 and SW620 cells with saikosaponin B4 solutions obtained above at 200 μg / mL, 100 μg / mL, 50 μg / mL, 25 μg / mL, 12.5 μg / mL, and 6.25 μg / mL, with 2 μL / mL of DMSO as the control; After 24h of SW480 cells, iffigure 1 As shown, in the concentration range of 25-200 μg / mL, the cell survival rate was significantly reduced, and the difference was significant compared with the normal control group (P<0.01). Compared with the control group, the cell survival rate was significantly lower (P<0.01), and the specific values are shown in Table 6; after saikosaponin B4 treated SW620 cells for 24h, 48h, and 72h, within the concentration range of 12.5-200μg / mL, compared with the normal control group, the cells The survival rate decreased significantly, and the difference was statistically significant (P<0.01). The specific values are shown in Table 7.
[0126] According to the experimental results of CCK8, the...
Embodiment 2
[0135] Example 2 Cell Morphology Observation
[0136] Observe the morphological changes of SW480 and SW620 cells treated with 25 μg / mL saikosaponin B4 (SSB4) for 24 hours. Such as figure 2 -a, Normal SW480 cells are irregular polygons, which fit closely to the bottom of the 6-well plate. When the 6-well plate is shaken, the cells do not shake with the medium. The cell outline is clear, the cytoplasm is clear, and the nucleus is clearly visible. SSB4( figure 2 -b) After treatment, the SW480 cells lose their polygonal shape and become round or oval. The cytoplasm becomes darker and no longer translucent. The cells will change from an adherent state to a semi-adherent or floating state. Shake the 6-well plate Suspended cells will shake with the culture medium.
[0137] Such as figure 2 -d, normal SW620 cells are in a regular spindle shape, the cell body is thick in the middle, narrow and slender at both ends, and also in an adherent state. SSB4( figure 2 -e) The SW620 c...
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