35 results about "Apoptosis related genes" patented technology

The invention discloses an application of mannitol in preparation of an anti-stomach cancer drug, and in particular relates to a use of a mannitol injection with a specific concentration of 10%-19% for treating stomach cancer. Traditionally, mannitol is only used as a diuretic, a high-permeability hypotensor and the like, while the inventor discovers through scientific experiments that mannitol has anti-tumor activity, and through several cell apoptosis detection technologies and by detecting apoptosis-related genes bcl-2, and bax, proves that the specific-concentration mannitol can induce various human cancer cell strains to generate obvious cancer cell apoptosis, grasps the effect relation with concentration and time, and first illustrates the possible mechanism; furthermore, the inventor has used the research result for tumor patient treatment and has achieved obvious curative effect, which provides theoretical basis and practical cases for the application of mannitol in the preparation of the anti-tumor drug and the clinical generalization thereof.

Provided are novel tetrazolo hydrazone derivatives or pharmaceutically acceptable salts thereof. The derivatives and the salts may inhibit the proliferation of cancer cells in a low molar concentration and inhibit the activity of cancer cells through the regulation of expression of apoptosis-related genes or the like, thus expressing excellent anti-cancer activity, and therefore, can be used effectively in preventing or treating cancer.

The application of theabrownin in the preparation of drugs for treating liver cancer belongs to the technical field of theabrownin use. Specifically include: the application of theabrownin in the preparation of drugs for treating liver cancer, theabrownin promotes the apoptosis of hepatocellular carcinoma Huh7 cells by activating the JNK signaling pathway, and inhibits the growth of liver cancer xenografts. The present invention proves through various tests that theabrownin has significant anti-proliferation and pro-apoptotic effects on Huh7 cells, and has dose-dependent effects. Theabrownin promotes the apoptosis of Huh7 cells and inhibits the growth of zebrafish liver cancer xenografts by activating the JNK signaling pathway. Theabrownin can inhibit tumor by 48.1%. Moreover, theabrownin regulates downstream apoptosis-related genes by activating the P53 pathway PUMA, NOXA, Bax, and Bcl‑2 and so on to promote the apoptosis of P53 wild Sk‑hep1 cells.

The invention relates to siRNA for inhibiting caspase-3 gene expression. The sequence thereof is as follows: the sense strand: 5'-GAGTCTGACTGGAAAGCCGAA-3'and the anti-sense strand: 5'-TTCGGCTTTCCAGGAAGACTC-3'. When entering nerve cells, the siRNA can obviously improve the vitality of nerve cells, obviously reduce the expression of apoptosis-related genes, i.e. caspase-3, of the nerve cells, lowerthe apoptosis and necrosis rate of the nerve cells and obviously reduce the expression of Alzheimer marker protein APP and that of Tau protein. The siRNA is capable of efficiently and specifically inhibiting the expression of disease-related gene caspase-3 so as to cause the silencing of relevant disease genes, and carrying out effective knockout on the expression of target genes to achieve the therapeutic purpose. The invention can be used for preparing medicines for inhibiting the apoptosis of the nerve cells or curing neurodegenerative diseases.