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Gene chip for detecting differential expression of human mitochondria gene

A mitochondrial gene and gene chip technology, applied in the direction of microbial measurement/testing, biochemical equipment and methods, etc., can solve the problems of expensive experiment costs and prohibitive

Inactive Publication Date: 2009-05-27
THE SECOND AFFILIATED HOSPITAL ARMY MEDICAL UNIV
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

But at present, people focus on the development of high-throughput gene chips, and its expensive experiment costs make people discouraged in scientific research.
At the same time, people's research on mitochondrial gene expression is limited to individual genes, lacking an overall understanding of the differential expression of mtDNA in different states and an analysis of its internal relationship

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  • Gene chip for detecting differential expression of human mitochondria gene
  • Gene chip for detecting differential expression of human mitochondria gene
  • Gene chip for detecting differential expression of human mitochondria gene

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Embodiment 1

[0048] Firstly, 13 kinds of mRNA encoded by mtDNA, 2 kinds of rRNA, 9 kinds of tRNA and 5 kinds of apoptosis-related genes encoded by nuclear gene (nDNA) were selected. Specifically, NADH-CoQ reductase subunit 1 (ND1), NADH-CoQ reductase subunit 2 (ND2), NADH-CoQ reductase subunit 3 (ND3), NADH-CoQ reductase subunit 4 (ND4) , NADH-CoQ reductase subunit 4L (ND4L), NADH-CoQ reductase subunit 5 (ND5), NADH-CoQ reductase subunit 6 (ND6), cytochrome b (Cytb) of complex III, complex Cytochrome oxidase subunit I (Cox I), cytochrome oxidase subunit II (Cox II), cytochrome oxidase subunit III (CoxIII) of IV, and ATPase subunit 6 of complex V (ATPase6) and ATPase subunit 8 (ATPase8 or ND6L), alanine transfer RNA (tRNA-Ala), day radon amide transfer RNA (tRNA-Asn), cysteine ​​transfer RNA (tRNA-Cys), glutamate transporter RNA (tRNA-Glu), serine transfer RNA (tRNA-Ser), heavy chain-encoded arginine transfer RNA (tRNA-Arg), aspartate transfer RNA (tRNA-Asp), glycine transfer RNA (tRNA-Gly...

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Abstract

The invention discloses a gene chip for detecting the differential expression of human mitochondrial genes. The gene chip is to a glass substrate incubated with probes for detecting the transcriptional abundance of 9 tRNAs, 2 rRNAs and 13 mRNAs encoded by human mitochondrial genes, as well as 5 apoptosis-associated genes encoded by nuclear DNA(nDNA). The gene chip provided by the invention is capable of detecting simultaneously the differential expression of the whole human mitochondrial mtDNA and nDNA under two different conditions, and analyzing the inherent correlation; and the invention represents the minimization and specialization of gene chip technology, and accomplishes the detection on expression of human mitochondrial mtDNA and nDNA efficiently and conveniently.

Description

technical field [0001] The invention belongs to an experimental tool for detecting the expression of human mitochondrial genes, in particular to an experimental tool for detecting the differential expression of human mitochondrial genes (mtDNA) and (nDNA) in two states. Background technique [0002] At present, the expression profile chip is currently the most widely used gene chip, which immobilizes as few as dozens to as many as thousands of specific probes on a carrier, which can be used to detect the transcription of many genes in the whole genome difference in abundance. The expression profile chip can analyze the differences in the abundance of mRNA transcripts from two or more groups of different sources, and obtain the information of differentially expressed genes by calculating the signal ratio and statistical analysis. But at present, people focus on the development of high-throughput gene chip, and its expensive experiment cost makes people daunted in scientific ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68
Inventor 胡义德孙恒文孙玉兰钱海洪
Owner THE SECOND AFFILIATED HOSPITAL ARMY MEDICAL UNIV
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