African swine fever virus chimeric protein, vaccine composition, preparation method and application thereof

A technology of African swine fever virus and vaccine composition, which is applied in the direction of biochemical equipment and methods, viruses, vaccines, etc., and can solve problems such as unsatisfactory vaccine immune effects

Active Publication Date: 2021-09-14
PU LIKE BIO ENG
View PDF5 Cites 4 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In order to solve the deficiencies in the prior art, the present invention provides a chimeric protein of African swine fever virus. When the chimeric protein of African swine fever v

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • African swine fever virus chimeric protein, vaccine composition, preparation method and application thereof
  • African swine fever virus chimeric protein, vaccine composition, preparation method and application thereof
  • African swine fever virus chimeric protein, vaccine composition, preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0057] Example 1 Expression of African swine fever virus p72 full-length and truncated proteins

[0058] The nucleotide sequence of the amino acid sequence of the p72 protein shown in SEQ ID NO.1 of the corresponding coding sequence table was synthesized by Suzhou Jinweizhi Biotechnology Co., Ltd., and the synthesized nucleotide was cloned into the pET28a vector, named as the recombinant plasmid pET28a-p72 .

[0059] Similarly, the African swine fever virus p72 domain I shown in the sequence table SEQ ID NO.2, the African swine fever virus p72 domain II shown in the sequence table SEQ ID NO.3, and the African swine fever virus shown in the sequence table SEQ ID NO.4 Pestivirus p72 domain III is connected by a flexible linker peptide, in which African swine fever virus p72 domain I is located at the N-terminus, domain III is located at the C-terminus, and domain II is located between domains I and III. Weizhi Biotechnology Co., Ltd. synthesized the corresponding coding nucleot...

Embodiment 2

[0063] Embodiment 2 The impact of African swine fever virus p72 truncated protein on immunogenicity

[0064] The p72 full-length protein and p72 truncated protein prepared in Example 1 were added to the adjuvant, and the process of adding was continuously stirred with an emulsifier at a speed of 800 rpm for 12 minutes, mixed, and stored at 4°C, that is, the p72 full-length protein or p72 truncated protein Vaccine compositions of p72 truncated proteins. The adjuvants suitable for the present invention may be adjuvants known to those skilled in the art. In the present invention, the adjuvant selected is a biphasic adjuvant (water-in-oil-in-water emulsion), for example, the adjuvant ISA206 (Seppic, France). The specific ratio of each component in the prepared vaccine is shown in Table 1.

[0065] Table 1 African swine fever virus p72 full-length and truncated protein vaccine composition composition ratio

[0066] components Vaccine 1 Vaccine 2 p72 protein (μ...

Embodiment 3

[0080] Embodiment 3 African swine fever virus chimeric protein backbone and antigenic protein selection

[0081] The inventor analyzed the structure of the African swine fever virus p72 protein and creatively truncated it, which still maintained the relative stability of the protein space structure, and its immunogenicity was not affected. Therefore, the p72 truncated protein was determined as the embedding protein of the present invention. The skeleton of the protein.

[0082] At the same time, after a large number of experimental studies, the inventors have screened the following sequence in combination with the antigenic epitope analysis of the African swine fever virus immunogenic protein. Fragments of at least one, to produce a higher immunogenic response:

[0083] The p54 protein fragment is selected from any one or more of SEQ ID NO.5, 6, 7, 8, 9, 10, 11, 12;

[0084] The p30 protein fragment is selected from any one or more of SEQ ID NO.13, 14, 15, 16, 17, 18, 19;

...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention provides an African swine fever virus chimeric protein. The chimeric protein comprises: (1) an African swine fever virus p72 structural domain I; (2) an African swine fever virus p72 structural domain II; (3) an African swine fever virus p72 structural domain III; and (4) an African swine fever virus antigenic protein. According to the chimeric protein provided by the invention, the African swine fever virus p72 protein is used as a skeleton for the first time, antigenic epitopes of African swine fever virus antigenic proteins p54, p30, CD2v and p12 are well displayed, the immune effect is good, and remarkable body fluid and cellular immunity can be generated.

Description

technical field [0001] The invention belongs to the technical field of veterinary biological products, and in particular relates to an African swine fever virus chimeric protein, a vaccine composition containing the chimeric protein, a preparation method and an application thereof. Background technique [0002] African swine fever (African swine fever, ASF) is an acute, severe and highly contagious infectious disease caused by African swine fever virus (ASFV), with a high incidence and a mortality rate of up to 100%. It is a kind of animal disease. The disease was first confirmed in Kenya in Africa in 1921. Since 2007, African swine fever has occurred, spread, and spread in many countries around the world. It was introduced to my country in 2018, causing huge direct and indirect economic losses. It has been nearly a hundred years since the disease was discovered, but there is currently no approved vaccine or specific drug in the world. [0003] The difficulty in the resear...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C07K19/00C12N15/62A61K39/295A61K39/12A61P31/20
CPCC07K14/005A61K39/12A61P31/20C07K2319/55C12N2710/12022C12N2710/12034A61K2039/70C12N15/62C07K19/00C12N15/63Y02A50/30A61K2039/55566A61K2039/5252C07K14/01A61K2039/552A61K2039/57C07K2319/00C12N2710/12052C12N2710/12071
Inventor 田克恭逄文强张许科
Owner PU LIKE BIO ENG
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap