Method for simultaneously extracting arabinogalactan and dihydroquercetin from larch

A technology of arabinogalactan and dihydroquercetin is applied in the field of separation and purification of natural products, and can solve the problems of long extraction time and high energy consumption.

Inactive Publication Date: 2021-09-14
BAISHENG BLUEBERRY TECH EXPLOITURE +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the traditional water bath extraction method has the disadvantages of long extraction time and high energy consumption

Method used

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  • Method for simultaneously extracting arabinogalactan and dihydroquercetin from larch

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] A method for simultaneously extracting arabinogalactan and dihydroquercetin from larch, comprising the following steps:

[0034] (1) pulverize the larch root to 10 meshes;

[0035] (2) Take 400 g of larch root powder, add 4000 g of 30% w / w ethanol, reflux for 1 time at 38 °C for 1.5 h, filter to obtain 3200 g of alcohol extract, and vacuum concentrate at 50 °C to obtain 320 g of concentrated alcohol extract The remaining larch root powder residue was added to 2400g of drinking water, refluxed for 1 time at 38°C for 1.5h, and filtered to obtain 2380g of water extract; the alcohol extract concentrate and the water extract concentrate were mixed to obtain 2700g total extraction liquid;

[0036] (3) 4 L of AB-8 macroporous resin was filled into the chromatography grease column, and the chromatography column was rinsed with 6 L of pure water; 2700 g of the total extract obtained in step (2) was adsorbed on the column, and the sample solution contained dihydroquercetin The ...

Embodiment 2

[0041] A method for simultaneously extracting arabinogalactan and dihydroquercetin from larch, comprising the following steps:

[0042] (1) pulverize the larch root to 10 meshes;

[0043] (2) Take 400g of larch root powder, add 4000g of 30%w / w ethanol, reflux for 1 time at 38°C for 1.5h, filter to obtain 3180g of alcohol extract, and vacuum concentrate at 50°C to obtain 315g of alcohol extract concentrate ; The remaining larch root powder residue was added to 2400g of drinking water, refluxed at 38°C for 1 time extraction, and the time was 1.5h, and filtered to obtain 2375g of water extract; The alcohol extract concentrate and the water extract concentrate were mixed to obtain 2690g total extraction liquid;

[0044] (3) 4 L of D-101 macroporous resin was filled into the chromatographic grease column, and the column was rinsed with 6 L of purified water; 2690 g of the total extract obtained in step (2) was adsorbed on the column, and the sample solution contained dihydroquerce...

Embodiment 3

[0049] A method for simultaneously extracting arabinogalactan and dihydroquercetin from larch, comprising the following steps:

[0050] (1) pulverize the larch root to 10 meshes;

[0051] (2) Take 400 g of larch root powder, add 4000 g of 30% w / w ethanol, reflux for 1 time at 38 °C for 1.5 h, filter to obtain 3220 g of alcohol extract, and concentrate under vacuum at 50 °C to obtain 305 g of concentrated alcohol extract ; The remaining larch root powder residue was added to 2400g of drinking water, refluxed for 1 time at 38°C for 1.5h, and filtered to obtain 2365g of water extract; the alcohol extract concentrate and the water extract concentrate were mixed to obtain 2670g total extraction liquid;

[0052] (3) 4 L of HPD100 macroporous resin was filled into the chromatography grease column, and the chromatography column was rinsed with 6 L of purified water; 2670 g of the total extract obtained in step (2) was adsorbed on the column, and the loading solution contained dihydro...

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Abstract

The invention discloses a method for simultaneously extracting arabinogalactan and dihydroquercetin from larch. Low-temperature operation is adopted in the whole process, and extraction is conducted in an alcohol extraction and water extraction combined extraction mode. On the basis of previous experimental research, the arabinogalactan and the dihydroquercetin are separated and purified by adopting macroporous adsorption resin column chromatography. The method comprises the steps of precipitating polysaccharide by using an alcohol precipitation method, detecting the impurity content of the purified substance by using a method of combining column chromatography with an ultraviolet and visible spectrophotometer, and determining the purity of the purified polysaccharide by using a phenol-sulfuric acid colorimetric method to determine the content of the active ingredients. The arabinogalactan is detected and quantitatively analyzed by fully utilizing a modern analysis instrument, so that high-purity arabinogalactan and dihydroquercetin products are obtained.

Description

technical field [0001] The invention relates to a method for simultaneously extracting arabinogalactan and dihydroquercetin from larch. The invention belongs to the technical field of natural product separation and purification. Background technique [0002] Larch is one of the main afforestation tree species in my country. In view of the current status of extraction and utilization of various bioactive components contained in the residue after felling and processing, we can recycle and reuse larch wood waste. . Optimizing the extraction process and developing the corresponding technologies for the effective utilization of active substances in wood are important measures to utilize larch plant resources, improve the industrial structure of forest areas, achieve the reuse of waste wood chips, and reduce production costs. [0003] A large amount of arabinogalactan exists in the xylem of larch, and its content can reach 5-30%. It is a neutral polysaccharide composed of arabino...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00C07D311/32C07D311/40
CPCC08B37/006C08B37/0003C07D311/32C07D311/40
Inventor 孙景波明月张雪
Owner BAISHENG BLUEBERRY TECH EXPLOITURE
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