Pediococcus pentosaceus HG-9 and application thereof

A technology of Pediococcus pentosaceus and HG-9, applied in the field of microorganisms, can solve problems such as side effects, increased risk of gallstones, renal dysfunction, etc.

Inactive Publication Date: 2021-09-14
BGI INST OF APPLIED AGRI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Statins and fibrates are commonly used clinically. The curative effect of the drugs is relatively significant at the initial stage, but with long-term use, side effects will occur to varying degrees, which will aggravate the health problems of patients. For example, long-term use of statins can easily induce myopathy , rhabdomyolysis, abnormal blood sugar, hepatitis and renal dysfunction, etc. Long-term use of fibrates can lead to serious liver damage an

Method used

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  • Pediococcus pentosaceus HG-9 and application thereof
  • Pediococcus pentosaceus HG-9 and application thereof
  • Pediococcus pentosaceus HG-9 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 3

[0066] The bacterial safety test of embodiment 3 Pediococcus pentosaceae HG-9

[0067] (1) Antibiotic susceptibility test

[0068] Antibiotic resistance of HG-9 was detected by microdilution method. Antibacterial drugs (Gentamicin, Kanamycin, Tetracycline, Erythromycin, Clindamycin, Chloramphenicol, Amplicilin) ​​will be tested at initial concentrations of 1024ug / mL, 256ug / mL, 64ug / mL, 8ug / mL, 16ug / mL, 64ug / mL, 16ug / mL / mL) do a series of 2-fold dilutions for a total of 12 times and add them to the corresponding wells of the 96-well plate, then add 2mL of the diluted test bacteria solution to the corresponding wells, observe after 48 hours of incubation, and use the minimum inhibitory concentration (MIC) of antibacterial drugs , which is the sensitivity of the test strain. The experimental results are shown in Table 1:

[0069] Table 1 The results of the resistance test of Pediococcus pentosaceae HG-9 to antibiotics

[0070] Antibiotics (mg / mL) HG-9 EFSA safe...

Embodiment 4

[0081] The in vitro acid resistance and bile resistance test of embodiment 4 Pediococcus pentosaceae HG-9

[0082] Prepare simulated gastric juice SGF (2.0g NaCl + 3.2g pepsin + appropriate amount of concentrated hydrochloric acid to adjust the pH value to 3.0), and filter it with a 0.2μm microporous membrane before use. Pick the strains from the slant and culture them in MRS liquid medium for 16-18 hours at 37°C. Centrifuge the bacterial suspension at 4000r / min for 15min, remove the supernatant, weigh the wet weight of the bacterial cells, resuspend the bacterial cells in normal saline at a ratio of 0.1g / mL, and add them to the SGF solution at a ratio of 1:10. After mixing well, place in a constant temperature incubator at 37°C and incubate for 2 hours to count the cells. See the experimental results figure 2 , the number of live bacteria remained at an order of magnitude, indicating that Pediococcus pentosaceae HG-9 has a strong acid resistance in vitro.

[0083] Insert ...

Embodiment 6

[0099] Preparation of a compound probiotic solid powder for regulating blood lipid. It consists of the following components in percentage by weight: 30% of lemon fruit powder, 22% of fructooligosaccharide, 20% of xylooligosaccharide, 20% of inulin, and 8% of Pediococcus pentosaceae HG-9 bacteria powder.

[0100] Animal experiments have proved that Pediococcus pentosaceae HG-9 can effectively reduce the levels of cholesterol and triglycerides in serum. Fructose-oligosaccharides, xylo-oligosaccharides and inulin are prebiotics, which can promote the proliferation of probiotics. In addition, many studies at home and abroad have shown that fructo-oligosaccharides, xylo-oligosaccharides and inulin can reduce the concentration of triglycerides and cholesterol in serum, thereby improving lipid metabolism. The formula also has the advantages of natural components, scientific formula, balanced nutrition, convenient taking, good solubility and non-sticky teeth.

[0101] The compound p...

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PUM

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Abstract

The invention relates to a pediococcus pentosaceus HG-9 and application thereof. The pediococcus pentosaceus HG-9 has been deposited in China Center for Type Culture Collection on April 6, 2021 under a deposit number of CCTCC No.2021329. The pediococcus pentosaceus HG-9 disclosed by the invention can be used for preparing food for reducing blood fat or medicines for reducing blood fat. The pediococcus pentosaceus HG-9 screened by the invention is relatively good in safety, can tolerate gastric acid and cholate, can survive and colonize in intestinal tracts, regulates and improves the intestinal microecological balance of a host, exerts beneficial effects of the host, and generates an exact healthy effect. In-vitro and animal experiments show that the pediococcus pentosaceus HG-9 has a relatively strong function of reducing triglyceride and cholesterol in blood.

Description

technical field [0001] The invention belongs to the technical field of microbes, and in particular relates to a strain of Pediococcus pentosaceae HG-9 and its application in blood lipid regulation. Background technique [0002] Hypercholesterolemia, also known as high and low-density lipoprotein cholesterolemia, is the primary risk factor leading to atherosclerotic cardiovascular disease. The latest research shows that for every 1mmol / L reduction in low-density lipoprotein in people with various risk of cardiovascular disease, major vascular events can be reduced by 21%. According to statistics in 2013, there are about 200 million people with borderline hypercholesterolemia and 88 million hypercholesterolemia patients in China. [0003] At present, drug therapy and dietary intervention are mainly used to control and reduce blood lipid levels. Statins and fibrates are commonly used clinically. The curative effect of the drugs is relatively significant at the initial stage, ...

Claims

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Application Information

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IPC IPC(8): C12N1/20A61K35/744A61P3/06A61P1/00A23L33/135C12R1/01
CPCA61K35/744A61P3/06A61P1/00A23L33/135A23V2002/00A23V2200/3262A23V2200/3204
Inventor 王明明代晓霜宋颀任军丽方晓东
Owner BGI INST OF APPLIED AGRI
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