Preparation method and application of skin cleaning composition containing yeast fermentation filtrate and natural plant extract
A technology of yeast fermentation and natural plants, which is applied in the field of preparation of skin cleansing compositions, can solve the problems of poor combination effect and incomplete effect, and achieve the effect of enhancing the cleaning effect
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Embodiment 1
[0030] Such as figure 1 Shown, a kind of preparation method of the skin cleansing composition of yeast fermentation filtrate and natural plant extract, comprises the following steps:
[0031] Step S1, activation of strains: thaw the frozen yeast strains and place them in the culture medium, and carry out activation culture under the fermentation conditions of pH range 5.5, fermentation temperature 28°C, shaker speed 210rpm, and fermentation time 24 hours .
[0032] Step S2, strain optimization: dilute the activated bacterial solution in step S1, and select the yeast with better growth condition on the wort medium to obtain a single colony.
[0033] Step S3, strain expansion: Inoculate the high-quality colony screened in step S2 into the liquid medium for expansion culture to obtain fermented yeast liquid.
[0034] Step S4, the formal fermentation stage: prepare a mixed solution with a concentration of 20g / L glucose, 20g / L peptone, 10g / L yeast extract, and 20g / L agar, and ins...
Embodiment 2
[0047] Such as figure 1 Shown, a kind of preparation method of the skin cleansing composition of yeast fermentation filtrate and natural plant extract, comprises the following steps:
[0048] Step S1, strain activation: thaw the frozen yeast strains and place them in the culture medium, and carry out activation culture under the fermentation conditions of pH range 5.8, fermentation temperature 34°C, shaker speed 240rpm, and fermentation time 36 hours .
[0049] Step S2, strain optimization: dilute the activated bacterial solution in step S1, and select the yeast with better growth condition on the wort medium to obtain a single colony.
[0050] Step S3, strain expansion: Inoculate the high-quality colony screened in step S2 into the liquid medium for expansion culture to obtain fermented yeast liquid.
[0051] Step S4, the formal fermentation stage: prepare a mixed solution with a concentration of 20g / L glucose, 20g / L peptone, 10g / L yeast extract, and 20g / L agar, and insert ...
Embodiment 3
[0064] Such as figure 1 Shown, a kind of preparation method of the skin cleansing composition of yeast fermentation filtrate and natural plant extract, comprises the following steps:
[0065] Step S1, strain activation: the frozen yeast strains were thawed and placed in the culture medium, and activated under the fermentation conditions of pH 6.0, fermentation temperature 25° C., shaker speed 220 rpm, and fermentation time 24 hours.
[0066] Step S2, strain optimization: dilute the activated bacterial solution in step S1, and select the yeast with better growth condition on the wort medium to obtain a single colony.
[0067] Step S3, strain expansion: Inoculate the high-quality colony screened in step S2 into the liquid medium for expansion culture to obtain fermented yeast liquid.
[0068] Step S4, the formal fermentation stage: prepare a mixed solution with a concentration of 20g / L glucose, 20g / L peptone, 10g / L yeast extract, and 20g / L agar, and insert 5% of the yeast solut...
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