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Alcaligenes of iron-producing carrier and application of alcaligenes

A technology of Alcaligenes faecalis and Alcaligenes faecalis, applied in the agricultural field, can solve problems such as reduction of plant resistance

Active Publication Date: 2021-09-21
NORTHWEST A & F UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Deficiency of soluble iron leads to reduced plant resistance

Method used

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  • Alcaligenes of iron-producing carrier and application of alcaligenes
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  • Alcaligenes of iron-producing carrier and application of alcaligenes

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Separating the soybean PGPR, producing siderophores ability to identify

[0017] 1, the source of soil samples

[0018] Rhizosphere experimental field of soybeans isolated from Zhengzhou, Henan Province.

[0019] 2, medium formula

[0020] (1) NA Medium

[0021] 3. Preparation of soil suspension

[0022] First shake off the dirt on a laboratory roots with sterile tweezers all nodules full off, rinsed with sterile water nodule 3 times, each time 3min, will not be washed away tightly adhered nodule bacterial cell surface . After all of the nodules into shake flasks containing glass beads and a 0.7% NaCl, 150rpm, shaken 1h 28 ℃. The suspension was diluted appropriately, applied on beef extract peptone medium.

[0023] 4, strain Purification

[0024] After the coating medium, the culture is placed under conditions 2-7d 28 ℃. During the incubation, the growth of colonies was observed, picked different forms, colors colonies were isolated and purified to obtain a pure culture. Le...

Embodiment 2

[0029] Strain identification soybean growth-promoting

[0030] This strain was cultured in a liquid medium 140rpm shaking for 2 days to extract the DNA, for 16s rDNA sequencing, sequences such as SEQ ID NO: 1.

[0031] The sequencing results BLAST alignment on NCBI, construct phylogenetic trees. Such as Figure 4 , The degree of similarity with the strain Alcaligenes faecalis subsp.Faecalis IAM12369 99%, belonging to the genus Alcaligenes lever (Alcaligenes sp.) Was named Alcaligenes sp.XPC26.

[0032] The strain of the genus Alcaligenes Alcaligenes sp.XPC26 lever 2020 December 28 deposited at the Chinese Culture Collection Committee General Microbiology Center (CGMCC), under the accession number CGMCC No: 21557, Category name: Alcaligenes Alcaligenes faecalis. Collection address: No. 3 Institute of microbial Beichen West Road, Chaoyang District, Beijing No. 1 Hospital.

Embodiment 3

[0034] 1, the strains tested

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Abstract

The invention discloses an alcaligenes strain of an iron-producing carrier and application of the alcaligenes strain, the alcaligenes strain is in China General Microbiological Culture Collection Center (CGMCC), the preservation number of the alcaligenes strain is CGMCC No: 21557, and the alcaligenes strain is classified and named as alcaligenes faecalis. The strain enables plants to better utilize the iron element to promote plant growth mainly through the iron-producing carrier, has important significance in improving the crop yield and reducing the use of chemical fertilizers and pesticides, and greatly promotes the development of green agriculture.

Description

Technical field [0001] The present invention relates to the field of agriculture, in particular, it relates to a production of siderophores Alcaligenes Its Applications. Background technique [0002] As the world population continues to grow rapidly increasing demand for food, in order to improve crop yields, frequently used in the production process of synthetic fertilizers and pesticides, causing serious environmental pollution, a serious impediment to the sustainable development of the ecosystem. Rhizosphere promoting bacteria (of PGPR) is one of the bacteria present in the soil and promote crop growth stimulation effect has, can partially substitute fertilizers and fertilizer and nicely complement each other in number and performance, to produce green, ensure food security. By studying rhizosphere promoting bacteria found PGPR often called biological fertilizer, comprising from Pseudomonas, Serratia, Bacillus, onion, Enterobacter, Alcaligenes, Arthrobacter spp species strain ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/20A01P21/00C12R1/05
CPCA01N63/20
Inventor 韦革宏李欣乐史鹏
Owner NORTHWEST A & F UNIV
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