Anti-cyclic citrullinated peptide antibody detection kit and method

Abstract

The invention discloses an anti-cyclic citrullinated peptide antibody detection kit, which comprises a pre-coating solution, and the pre-coating solution is provided with a streptavidin and carbon nanotube combination body. The method comprises the following steps: firstly, adding a pre-coating solution into a coating hole for pre-coating, and then adding a coating solution into a hole of a coating plate; obtaining the coated plate which is coated with the anti-cyclic citrullinated peptide antigen-streptavidin-carbon nano tube; then adding a horse radish peroxidase goat anti-rabbit I gG reagent; then adding an o-phenylenediamine display substrate reagent, adding a sulfuric acid termination reagent, and finally recording readings by using an enzyme-linked immunosorbent assay instrument. By introducing streptavidin, the loading effect of the pre-coating liquid on a coated plate is enhanced, so that the pre-coating liquid is loaded on a plate body in a more uniform and dispersed manner, the binding rate and effect of the pre-coating liquid on the coated plate are improved, and the time of the subsequent coating step is shortened, and moreover, the sensitivity is correspondingly improved.

Description

technical field [0001] The invention specifically relates to an anti-cyclic citrullinated peptide antibody detection kit and method. Background technique [0002] Anti-cyclic citrullinated peptide antibody is a biological antibody capable of reacting and synthesizing cyclic citrullinated polypeptide (CCP) as an antigen. This antibody can be a good indicator of the specificity of rheumatoid arthritis. Therefore, It can be used as one of the important indicator substances for detecting and diagnosing the disease at an early stage. In clinical practice, enzyme-linked immunosorbent assay (ELISA) is generally used to detect this antibody. [0003] When actually using enzyme-linked immunosorbent assay (ELISA) to detect anti-cyclic citrullinated peptide antibodies, one of the problems needs special attention, that is, the detection sensitivity is insufficient, and some positive samples are prone to problems that cannot be detected. In addition, It is just that the time required f...

AI Technical Summary

Problems solved by technology

[0003] When actually using enzyme-linked immunosorbent assay (ELISA) to detect anti-cyclic citrullinated peptide antibodies, one of the problems needs special attention, that is, the detection sensitivity is insufficient, and some positive samples are prone to problems that cannot be detected. In addition, It is the process of coating, that is, coating the anti-cyclic citrullinated peptide antigen on the polystyrene plate, which takes too long, usually up to 20 hours, which obviously reduces the work efficiency

The reason is that the molecular weight of the antigen is insufficient, its binding rate with the polystyrene carrier is slow and the dispersion is not enough, resulting in the inability to complete the binding or difficult binding at some sites, thus causing the existence of the above-mentioned technical problems