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Ex-vivo propagation method for eucommia ulmoides mature embryos

A technology for in vitro reproduction and mature embryos, applied in horticultural methods, botanical equipment and methods, planting substrates, etc., to achieve the effects of increasing germination rate, accelerating the formation of true leaves, and uniform growth potential

Active Publication Date: 2021-10-08
河南龙源风景园林建设有限公司
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Problems solved by technology

[0004] For a long time, due to the destruction of the natural environment, unrestrained excavation, and the technical problems of cutting propagation have not been completely resolved, the supply of Eucommia seedlings in the market is in short supply, and there is a big gap for excellent plants. One of the effective methods to solve the rapid propagation of seedlings

Method used

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  • Ex-vivo propagation method for eucommia ulmoides mature embryos
  • Ex-vivo propagation method for eucommia ulmoides mature embryos
  • Ex-vivo propagation method for eucommia ulmoides mature embryos

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Embodiment 1

[0031] A method for in vitro propagation of eucommia mature embryos, comprising the following steps:

[0032] (1) Taking Eucommia ulmoides plants in Henan Institute of Science and Technology as the test material, take the mature seeds of the year from the female parent plant, wash them with clean water 3-5 times, then soak them in carbendazim for 10 minutes, sterilize them with 75% alcohol for 1 minute, and finally 0.1% HgCl 2 Disinfect for 10 minutes, wash with sterile water 3-5 times, peel off the seed coat, use mature embryos as explants, inoculate on MS medium (the MS medium contains 0.2mg / L 6-BA, 0.1mg / L L NAA, 0.5mg / L GA 3 , 3% sucrose, 0.8% agar, pH 5.8-6.0), placed at 20-24°C, light intensity 700-800lx, and light time 14h / d for cultivation (observation records during the period, the germination rate can reach 100%) , when growing to the first pair of true leaves fully unfolded, transfer to 1 / 2MS medium (the 1 / 2MS medium contains 3% sucrose, 0.8% agar, pH is 5.8-6.0) ...

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Abstract

The invention discloses an ex-vivo propagation method for eucommia ulmoides mature embryos, which is mainly applied to ex-vivo rapid propagation and germplasm innovation research of eucommia ulmoides. The method comprises the following steps: selecting current-year mature eucommia ulmoides seeds, disinfecting by using a proper method, peeling off seed coats, culturing by taking mature embryos as explants, performing rooting induction, and finally completing a series of domestication processes. According to the method, the advantage of uniform growth of ex-vivo culture is utilized, ex-vivo culture is carried out on the mature embryos of eucommia ulmoides, a systematic method suitable for ex-vivo culture of the mature embryos of eucommia ulmoides is screened out, then an effective way is provided for germplasm innovation, and a large number of aseptic seedlings can be provided as the explants for rapid propagation.

Description

technical field [0001] The invention belongs to the technical field of in vitro rapid propagation and germplasm innovation of eucommia ulmoides, in particular to a method for in vitro propagation of mature embryos of eucommia ulmoides. Background technique [0002] Eucommia ulmoides Oliv., also known as Bakelite, is a single species of Eucommia ulmoides. It is a unique economic tree species in my country and has unique medicinal value. It has a two-way regulating effect on blood pressure and is considered to be a highly effective natural antihypertensive drug in the world. . In addition, Eucommia ulmoides is also a kind of glue source plant. Eucommia ulmoides gum has good acid and alkali resistance, insulation, thermoplasticity and shape memory, and is one of the most promising glue source plants in the temperate zone. [0003] my country is the largest demand country for natural rubber in the world, but at present, the annual output of clover rubber, the main raw material o...

Claims

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Application Information

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IPC IPC(8): A01H4/00A01G31/00A01G24/15
CPCA01H4/008A01H4/002A01G31/00A01G24/15
Inventor 李捷王继海李鹏赵建团杜硕丰吴俊汤永军李霞黄一远张翔宇常珍惜
Owner 河南龙源风景园林建设有限公司
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