High-temperature-resistant kitchen waste decomposing complex microbial inoculant and preparation method and application thereof

A technology of food waste and compound bacterial agents, applied in biochemical equipment and methods, methods based on microorganisms, bacteria, etc., can solve problems such as ignoring the cultivation process of the bacteria bed, incomplete consideration of antagonism, and complex components of food waste

Pending Publication Date: 2021-10-08
ZHEJIANG YANHANG INTELLIGENT TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0011] (1) The components of food waste are complex, and the microbial compound agent has a single function, which limits the scope of use and leads to poor treatment effects
[0012] (2) The compounding of microbial compound bacterial agents does not fully consider the appropriate ratio between strains and the antagonism between strains, resulting in unstable product quality and use effects, and related problems emerge in endlessly
[0013] (3) There is a high-temperature stage in the aerobic composting process of food waste, and the temperature can reach 60-70°C, so that harmful bacteria and insect eggs can be killed. Bacteria that cannot tolerate high temperatures cannot survive and reproduce, and ultimately affect the degradation effect
[0014] (4) During the actual use of microbial compound bacterial agents, most people will ignore the cultivation process of the bacterial bed, and rarely add bacterial agent attractants

Method used

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  • High-temperature-resistant kitchen waste decomposing complex microbial inoculant and preparation method and application thereof
  • High-temperature-resistant kitchen waste decomposing complex microbial inoculant and preparation method and application thereof
  • High-temperature-resistant kitchen waste decomposing complex microbial inoculant and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0092] The invention provides a high-temperature-resistant food waste decomposing composite bacterial agent, comprising:

[0093] Bacillus thuringiensis, Bacillus licheniformis, Geobacillus stearothermophilus, Bacillus subtilis, actinomycetes, Mold cinerea, Candida tropicalis; the volume ratio of each component is 6 parts of Bacillus thuringiensis, 2 parts of Bacillus licheniformis 1 part, 1 part of Geobacillus stearothermophilus, 6 parts of Bacillus subtilis, 1 part of Actinomycetes, 2 parts of Cinereus cinerea, 6 parts of Candida tropicalis Bacillus mycoides.

Embodiment 2

[0095] The invention provides a method for preparing a high-temperature-resistant food waste decomposing compound bacterial agent, which includes the following steps of S1 to S7

[0096] Before the present invention is prepared, various mediums need to be prepared earlier, and the formula of the medium is as follows:

[0097] LB medium formula: tryptone (Tryptone) 10g / L, yeast extract (Yeast extract) 5g / L, sodium chloride (NaCl) 10g / L, add 15g~20g agar powder, distilled water 1000ml, pH7.4, Sterilize at 121°C for 20 minutes.

[0098] Gao's No. 1 medium formula: soluble starch (20g), KNO3 (1g), K2HPO4 (0.5g), MgSO4 7H2O (0.5g), NaCl (0.5g), FeSO4 7H2O (0.01g), agar 20g, pH=7.4-7.6.

[0099] MEA medium: 30g malt extract powder, 15.0g agar, 3.0g soytone, pH5.4-5.8, sterilized at 121°C for 20min.

[0100] PDA medium: 200g potato, 20g glucose, 15-20g agar, sterilized at 115°C for 20min.

[0101] Degraded starch medium: soluble starch 20.0g, peptone 5.0g, sodium chloride (NaCI) ...

Embodiment 3

[0206] The present invention also provides an application of a high-temperature-resistant food waste decomposing compound bacterial agent, which includes putting the prepared high-temperature-resistant food waste decomposing compound bacterial agent in a liquid state or a solid state into the kitchen according to the above preparation method. Biochemical fermentation deodorization is carried out in the garbage biochemical fermentation bin, or the high-temperature resistant food waste decomposing compound bacterial agent is mixed into the compost after leaving the bin for biochemical fermentation deodorization. The application example of solid bacterial agent on kitchen waste is as follows

[0207] Screen three kinds of decomposing agents T1, T2 and T3 that are better sold in the market, among which T1 is Shuguo food waste degrading bacteria agent, T2 is Qiangxing compost starter, T3 is Gymboree fertilizer starter. The raw materials are processed according to the aerobic compos...

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Abstract

The invention discloses a high-temperature-resistant kitchen waste decomposing complex microbial inoculant and a preparation method and application thereof. The high-temperature-resistant kitchen waste decomposing complex microbial inoculant of the invention is prepared from the following components: 6 parts of bacillus thuringiensis, 2 parts of bacillus licheniformis, 1 part of geobacillus stearothermophilus, 6 parts of bacillus subtillis, 1 part of actinobacillus brumpt, 2 parts of taifanglania cinerea and 6 parts of candida tropicalis. What is finally prepared by the invention is the kitchen waste decomposed solid complex microbial inoculant resistant to the high temperature of 60-70 DEG C, the microbial inoculant can kill harmful germs and worm eggs without influencing the activity of strains at the high temperature, and meanwhile, a microbial inoculant attractant is added, so that the number of the strains is increased, namely the time spent is shortened under the same number of strains; the bacillus thuringiensis is also added, and crystal protein of the bacillus thuringiensis has a poisoning effect on insects such as mosquitoes and flies and does not have an inhibiting effect on bacteria; and the strains contained in the complex microbial inoculant are all screened from kitchen waste biochemical bin samples, so that the complex microbial inoculant does not cause harm to the surrounding environment and ecological balance.

Description

technical field [0001] The invention belongs to the technical field of organic waste treatment, and in particular relates to a high-temperature-resistant food waste decomposing composite bacterial agent and a preparation method and application thereof. Background technique [0002] Food waste is a typical solid waste, which has a high moisture content and poor dehydration performance. It is easy to rot at high temperature and breed germs and mosquitoes. and other organic matter, but has comprehensive and rich nutrients, so it has strong biodegradability. In view of this, the use of biological methods to treat food waste has gradually become one of the main development directions for resourceful treatment of food waste, which is effective and timely Proper treatment of kitchen waste can also greatly alleviate environmental pollution and improve people's living environment. Now, how to successfully use biomass resources as a daily energy source has become a research hotspot t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/16C12N1/14C12N1/02C12N11/14C12N11/02C05F9/04C12R1/07C12R1/10C12R1/125C12R1/04C12R1/645C12R1/74
CPCC12N1/20C12N1/16C12N1/14C12N1/02C12N11/14C12N11/02C05F9/04Y02A40/20
Inventor 付鹏睿张辉肖通奋徐沈利刘江涛
Owner ZHEJIANG YANHANG INTELLIGENT TECH CO LTD
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