Systematic reaction liquid, quantitative detection kit for 25-hydroxyvitamin D and use method of the kit
A technology for the quantitative detection of hydroxyvitamins, which is applied in the field of immunological detection, and can solve problems such as wrong sample test results, abnormal binding proteins, and interference with competitive reactions.
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[0028] A system reaction liquid, including EDTA, sodium dodecyl sulfate, dimethyl sulfoxide and ethanol.
[0029] The system reaction liquid, EDTA is EDTA-2K, and each liter contains 5.8-7g of EDTA-2K, 1.5-2.5g of sodium dodecylsulfonate, 90ml-110ml of dimethyl sulfoxide and 45ml-55ml of ethanol.
[0030] The system reaction solution also includes a buffer and a preservative.
[0031] For the system reaction solution, the buffer is Tris-NaCl buffer or PBS buffer, and the preservative is at least one of Proclin300, NaN3, and Cathone.
[0032] A 25-hydroxyvitamin D quantitative detection kit, comprising the system reaction solution described in the claims, also comprising a coated plate coated with 25(OH)VD-BSA, an enzyme containing an enzyme-labeled 25(OH)VD monoclonal antibody Calibrators for conjugates and 25(OH)VD.
[0033] The dilution of the enzyme conjugate contains casein.
[0034] The diluent of the enzyme conjugate includes a buffer and a preservative, the buffer is...
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