Systematic reaction liquid, quantitative detection kit for 25-hydroxyvitamin D and use method of the kit

Abstract

The invention provides a systematic reaction liquid, a quantitative detection kit for 25-hydroxyvitamin D and a use method of the kit, and the systematic reaction liquid comprises EDTA, sodium dodecyl sulfate, dimethyl sulfoxide and ethanol. The quantitative detection kit for the 25-hydroxyvitamin D comprises the system reaction liquid as described in the claims, and further comprises a coating plate coated with 25 (OH)VD-BSA, an enzyme conjugate containing an enzyme-labeled 25(OH)VD monoclonal antibody and a 25(OH)VD calibrator. In the detection process, different effective components of new and old samples are eliminated, and effective components interfered by HAMA samples are eliminated, so that the quantitative detection kit for the 25-hydroxyvitamin D provided by the invention achieves quicker and more convenient operation and obtains more accurate detection results.

Description

technical field [0001] The invention relates to the technical field of immunological detection, in particular to a system reaction solution, a 25-hydroxyvitamin D quantitative detection kit and a use method thereof. Background technique [0002] Vitamin D (vitamin D) is a steroid hormone with anti-rickets effect, also known as anti-rickets vitamin. Vitamin D is a derivative of different provitamin D after ultraviolet irradiation. Vitamin D is a fat-soluble vitamin with five compounds, among which vitamin D2 (ergocalciferol) and vitamin D3 (cholecalciferol) are more closely related to health. It exists in some natural foods, and 7-dehydrocholesterol produced from cholesterol is stored under the skin of the human body, which can be converted into vitamin D after being irradiated by ultraviolet rays. When vitamin D is transported to the liver, it acts on the monooxygenase system in microsomes, and its 25-position is hydroxylated to form 25(OH)VD. Other tissues outside the liv...

AI Technical Summary

Problems solved by technology

For example, companies such as Beijing Bohui Innovative Biotech, Guangzhou Feikang Biology, Beijing Meikang Biology, and Beijing Zhengdan International Technology all use this method. This method requires a separate step of sample pretreatment. The important thing is that this method is easy to cause A certain percentage of samples with abnormal protein binding results are falsely detected

The reason is that the binding protein of these samples has the opportunity to form a new combination with the enzyme-labeled 25-hydroxyvitamin D antigen after dissociation, resulting in the consequence of interfering with the competition reaction

Images