Eureka AIR delivers breakthrough ideas for toughest innovation challenges, trusted by R&D personnel around the world.

Enzyme conjugate diluent, total thyroxine quantitative detection kit and use method of total thyroxine quantitative detection kit

An enzyme conjugate, thyroxine technology, applied in the field of immunological detection, can solve the problems of interference competition reaction, wrong detection results of binding protein samples, etc.

Pending Publication Date: 2021-10-12
郑州达诺生物技术有限公司
View PDF14 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For example: Beijing Kemei Dongya Company, Beijing Yuande Biology, Beijing Meikang Biology, Zhengzhou Antu Biology and other companies all use this method. This method requires a separate step of sample pretreatment. The important thing is that this method is easy Causes a certain percentage of samples with abnormal binding proteins to have false detection results
The reason is that the binding proteins of these samples have the opportunity to form new combinations with the enzyme-labeled thyroxine (T4) antigen after dissociation, resulting in interference with the competition reaction

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Enzyme conjugate diluent, total thyroxine quantitative detection kit and use method of total thyroxine quantitative detection kit
  • Enzyme conjugate diluent, total thyroxine quantitative detection kit and use method of total thyroxine quantitative detection kit
  • Enzyme conjugate diluent, total thyroxine quantitative detection kit and use method of total thyroxine quantitative detection kit

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0041] A method for preparing a coated plate coated with thyroxine, comprising the following steps: (1) adding T4-BSA to tris-HCl buffer solution to prepare a coating solution, and fully mixing;

[0042](2) Add the prepared coating solution into a blank microtiter plate and place it at 2-8°C for 16-24hr;

[0043] (3) Shake off the coating solution in the microplate, and wash the plate at least twice with washing solution after there is no liquid on the surface of the microplate;

[0044] (4) Seal within 2 minutes after washing the plate, then add blocking solution to each well, and then place it at 2-8°C for 16-24 hours;

[0045] (5) Shake off the blocking solution of the microplate, and then place the microplate in a drying device to dry (humidity ≤ 30%) for 4-8 hours.

[0046] The preparation method of thyroxine calibrator comprises the following steps: (1) using HEPES buffer solution, BSA, nystatin and Proclin300 with pH of 6.8-7.5 to prepare calibrator dilution;

[0047]...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The present invention provides an enzyme conjugate diluent, a total thyroxine quantitative detection kit and a use method of a total thyroxine quantitative detection kit, the enzyme conjugate diluent provided by the invention can enhance the stability of an enzyme conjugate and inhibit the destructive effect of a dissociation agent on the enzyme conjugate, so that the enzyme conjugate and the dissociation agent can coexist in the same reagent component, in total thyroxine detection, total thyroxine dissociation and reduction of interference of new and old samples and HAMA interference can be met at the same time, so that the total thyroxine quantitative detection kit provided by the invention achieves quicker and more convenient operation and obtains more accurate detection results.

Description

technical field [0001] The invention relates to the technical field of immunological detection, in particular to an enzyme conjugate diluent, a total thyroxine quantitative detection kit and a use method thereof. Background technique [0002] T4 (L-thyroxine) is a hormone synthesized and stored in the thyroid gland. Proteases hydrolyze follicular thyroglobulin, releasing T4 into the blood. 99.97% of T4 in human blood is reversibly bound to three plasma proteins - thyroid-binding globulin (TBG) binds about 70% of T4, and the remaining about 30% of T4 binds to thyroid-binding prealbumin (TBPA) and albumin, any At all times, about 0.03% of T4 is in a free unbound state in the blood. [0003] The existing detection methods for total thyroxine (TT4) include liquid chromatography-tandem mass spectrometry and immunological methods. Among them, the immunological method is the mainstream method in clinical application because of its fast detection and low cost. On the platform, c...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): G01N33/78G01N33/577
CPCG01N33/78G01N33/577
Inventor 王玲玲普鹏
Owner 郑州达诺生物技术有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com