Enzyme conjugate diluent, total thyroxine quantitative detection kit and use method of total thyroxine quantitative detection kit
An enzyme conjugate, thyroxine technology, applied in the field of immunological detection, can solve the problems of interference competition reaction, wrong detection results of binding protein samples, etc.
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[0041] A method for preparing a coated plate coated with thyroxine, comprising the following steps: (1) adding T4-BSA to tris-HCl buffer solution to prepare a coating solution, and fully mixing;
[0042](2) Add the prepared coating solution into a blank microtiter plate and place it at 2-8°C for 16-24hr;
[0043] (3) Shake off the coating solution in the microplate, and wash the plate at least twice with washing solution after there is no liquid on the surface of the microplate;
[0044] (4) Seal within 2 minutes after washing the plate, then add blocking solution to each well, and then place it at 2-8°C for 16-24 hours;
[0045] (5) Shake off the blocking solution of the microplate, and then place the microplate in a drying device to dry (humidity ≤ 30%) for 4-8 hours.
[0046] The preparation method of thyroxine calibrator comprises the following steps: (1) using HEPES buffer solution, BSA, nystatin and Proclin300 with pH of 6.8-7.5 to prepare calibrator dilution;
[0047]...
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