Tissue culture and rapid propagation method for watermelons

A technology for tissue culture rapid propagation and watermelon, applied in horticultural methods, botanical equipment and methods, cultivation and other directions, can solve the problem of low survival rate of grafting, low survival rate of tissue culture seedling transplanting, and immature development of tissue culture propagation technology. and other problems, to achieve the effect of stable genetic inheritance and high reproduction rate

Inactive Publication Date: 2021-10-19
优奈尔生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Early spring red jade watermelon is a popular fruit with high economic value, but at present domestic early spring red jade watermelon is mainly propagated by seeds, and the development of tissue culture propagation technology is still immature
[0005] The early spring red jade watermelon tissue culture propagation technology has the following technical defects: the transplanting survival rate of tissue culture seedlings is not high, and the grafting survival rate is low

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Embodiment 1 A kind of watermelon tissue culture rapid propagation method

[0038] Including the following steps:

[0039] (1) Selection of explants

[0040]Grow early spring ruby ​​watermelons by planting from seed. Select robust, disease-free watermelon plants with a main vine length of 1.8m, and cut the tip of the stem at 1.5cm as an explant.

[0041] (2) After-treatment in vitro

[0042] Transfer the explants to a tissue culture laboratory for ex vivo post-processing. The in vitro post-treatment includes disinfection treatment and immersion treatment. The disinfection treatment: put the explants in a jar, add 0.08% mercuric chloride for disinfection for 40 seconds, then rinse with sterile water for 3 times, then add 75% alcohol for disinfection 20s, then rinse 3 times with sterile water. The soaking treatment: mix magnesium sulfate, potassium dihydrogen phosphate, calcium chloride and sterile water according to the mass ratio of 2:0.5:1:200 to obtain a mixed s...

Embodiment 2

[0065] Embodiment 2 A kind of watermelon tissue culture rapid propagation method

[0066] Including the following steps:

[0067] (1) Selection of explants

[0068] Grow early spring ruby ​​watermelons by planting from seed. Select robust, disease-free watermelon plants with a main vine length of 1.6m, and cut the tip of the stem at 1.3cm as an explant.

[0069] (2) After-treatment in vitro

[0070] Transfer the explants to a tissue culture laboratory for ex vivo post-processing. The in vitro post-treatment includes disinfection treatment and immersion treatment. The disinfection treatment: put the explants in a jar, add 0.08% mercuric chloride for disinfection for 45 seconds, then rinse with sterile water for 3 times, then add 75% alcohol for disinfection 20s, then rinse 3 times with sterile water. The soaking treatment: mix magnesium sulfate, potassium dihydrogen phosphate, calcium chloride and sterile water according to the mass ratio of 2:0.5:1:200 to obtain a mixed ...

Embodiment 3

[0092] Embodiment 3 A kind of watermelon tissue culture rapid propagation method

[0093] Including the following steps:

[0094] (1) Selection of explants

[0095] Grow early spring ruby ​​watermelon by planting from seed. Select strong, disease-free watermelon plants with a main vine length of 1.5m, and cut the tip of the stem 1cm as an explant.

[0096] (2) After-treatment in vitro

[0097] Transfer the explants to a tissue culture laboratory for ex vivo post-processing. The in vitro post-treatment includes disinfection treatment and immersion treatment. The disinfection treatment: put the explants in a jar, add 0.08% mercuric chloride for disinfection for 50 seconds, then rinse with sterile water for 3 times, then add 75% alcohol for disinfection 20s, then rinse 3 times with sterile water. The soaking treatment: mix magnesium sulfate, potassium dihydrogen phosphate, calcium chloride and sterile water according to the mass ratio of 2:0.5:1:200 to obtain a mixed soluti...

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Abstract

The invention provides a tissue culture and rapid propagation method for watermelons. The tissue culture and rapid propagation method comprises the steps of explant selection, in-vitro post-treatment, dedifferentiation, differentiation culture, strong seedling culture, grafting and seedling hardening. In the step of explant selection, an explant is a stem tip, and the length of the explant is 1-1.5 cm; in the differentiation culture, the culture time is 20-24 days; the seedling hardening comprises the steps of transplanting tissue culture grafted seedlings into a culture medium, covering a film and hardening the seedlings for 20 days, and hardening the seedlings in the first ten days: the illumination intensity is 2800-2900 Lx, the illumination time is 12-14 hours, the temperature is controlled to be 27-28 DEG C in the daytime and 17-20 DEG C at night, and the humidity is controlled to be 67-70%; and the seedling hardening in the last ten days: illumination intensity is 3000-3100 Lx, the illumination time is 10-11 h, the temperature is controlled to be 31-32 DEG C in the daytime and 12-15 DEG C at night, and the humidity is controlled to be 60%-63%. The transplanting survival rate and the grafting survival rate of the tissue culture seedlings are greatly increased.

Description

technical field [0001] The invention belongs to the field of plant tissue culture propagation, and in particular relates to a watermelon tissue culture rapid propagation method. Background technique [0002] Watermelon, monoecious. Both male and female flowers are solitary in leaf axils. Male flowers: Pedicels 3-4 cm long, densely covered with yellow-brown villous hairs; calyx tube broadly campanulate, densely villous, calyx lobes narrowly lanceolate, nearly as long as calyx tube, 2-3 mm long; corolla Pale yellow, 2.5-3 cm in diameter, greenish outside, villous, lobes ovate-oblong, 1-1.5 cm long, 0.5-0.8 cm wide, blunt or slightly pointed apex, yellowish-brown veins, hairy; Stamens 3, nearly free, 1 with 1 cell, 2 with 2 cells, filaments are short, and the cells are curved. Female flowers: calyx and corolla are the same as androgynous flowers; ovary ovate, 0.5-0.8 cm long, 0.4 cm wide, densely villous, styles 4-5 mm long, stigmas 3, reniform. [0003] Tissue culture prop...

Claims

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Application Information

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IPC IPC(8): A01H4/00A01G2/30A01G31/00A01G22/05
CPCA01H4/005A01G2/30A01G31/00A01G22/05
Inventor 金炳奎由守昌王昌盛杨猛李宁刘金宝
Owner 优奈尔生物科技有限公司
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