Tissue culture and rapid propagation method for watermelons

Abstract

The invention provides a tissue culture and rapid propagation method for watermelons. The tissue culture and rapid propagation method comprises the steps of explant selection, in-vitro post-treatment, dedifferentiation, differentiation culture, strong seedling culture, grafting and seedling hardening. In the step of explant selection, an explant is a stem tip, and the length of the explant is 1-1.5 cm; in the differentiation culture, the culture time is 20-24 days; the seedling hardening comprises the steps of transplanting tissue culture grafted seedlings into a culture medium, covering a film and hardening the seedlings for 20 days, and hardening the seedlings in the first ten days: the illumination intensity is 2800-2900 Lx, the illumination time is 12-14 hours, the temperature is controlled to be 27-28 DEG C in the daytime and 17-20 DEG C at night, and the humidity is controlled to be 67-70%; and the seedling hardening in the last ten days: illumination intensity is 3000-3100 Lx, the illumination time is 10-11 h, the temperature is controlled to be 31-32 DEG C in the daytime and 12-15 DEG C at night, and the humidity is controlled to be 60%-63%. The transplanting survival rate and the grafting survival rate of the tissue culture seedlings are greatly increased.

Description

technical field [0001] The invention belongs to the field of plant tissue culture propagation, and in particular relates to a watermelon tissue culture rapid propagation method. Background technique [0002] Watermelon, monoecious. Both male and female flowers are solitary in leaf axils. Male flowers: Pedicels 3-4 cm long, densely covered with yellow-brown villous hairs; calyx tube broadly campanulate, densely villous, calyx lobes narrowly lanceolate, nearly as long as calyx tube, 2-3 mm long; corolla Pale yellow, 2.5-3 cm in diameter, greenish outside, villous, lobes ovate-oblong, 1-1.5 cm long, 0.5-0.8 cm wide, blunt or slightly pointed apex, yellowish-brown veins, hairy; Stamens 3, nearly free, 1 with 1 cell, 2 with 2 cells, filaments are short, and the cells are curved. Female flowers: calyx and corolla are the same as androgynous flowers; ovary ovate, 0.5-0.8 cm long, 0.4 cm wide, densely villous, styles 4-5 mm long, stigmas 3, reniform. [0003] Tissue culture prop...

AI Technical Summary

Problems solved by technology

Early spring red jade watermelon is a popular fruit with high economic value, but at present domestic early spring red jade watermelon is mainly propagated by seeds, and the development of tissue culture propagation technology is still immature

[0005] The early spring red jade watermelon tissue culture propagation technology has the following technical defects: the transplanting survival rate of tissue culture seedlings is not high, and the grafting survival rate is low