Sample detection device and method

Abstract

The invention relates to a sample detection device and method. The sample detection device comprises a reagent tube, a tube cover, a reaction container and a magnetic sleeve. One end of the reagent tube is provided with an opening, and the reagent tube is internally provided with at least a cracking cavity and an elution cavity. The tube cover is mounted at the opening end of the reagent tube and is rotatably connected with the reagent tube. The tube cover is provided with a first sample adding hole and a penetrating hole. The magnetic sleeve is inserted into the insertion hole, can move in the direction close to or away from the reagent tube, and is used for being matched with the magnetic rod to attract and release the magnetic beads. The reaction container is provided with a reaction cavity, and liquid in the elution cavity can flow into the reaction cavity. Therefore, the pretreatment of a to-be-detected sample and the separation, extraction and detection of the target substance are integrated in the reagent tube, seamless joint of the whole process is realized, and the reagent tube is convenient to use. In addition, the to-be-detected sample is prepared and detected in a closed space, so that the probability of pollution of the to-be-detected sample is reduced, the detection reliability is ensured, and the biological hazard is reduced.

Description

technical field [0001] The invention relates to the technical field of biological detection, in particular to a sample detection device and method. Background technique [0002] In medical testing, molecular detection is mainly used in the detection of pathogenic microorganism infectivity and so on. The mainstream technology of molecular detection is fluorescent quantitative PCR (polymerase chain reaction) technology. Due to the characteristics of PCR technology exponentially amplifying templates, the factors such as aerosol pollution and complicated operation have limited the further application of fluorescent quantitative PCR technology. in clinical medicine. [0003] Routine experimental operations are mostly carried out in an open experimental environment. The samples to be tested can be in direct contact with the outside world through the air, but there are often some samples that are harmful to the operator, easily disturbed by the outside world, or interfere with the...

AI Technical Summary

Problems solved by technology

[0003] Routine experimental operations are mostly carried out in an open experimental environment. The samples to be tested can be in direct contact with the outside world through the air, but there are often some samples that are harmful to the operator, easily disturbed by the outside world, or interfere with the outside world. diseased biological samples

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