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Speckle fluorescence microscopy method and system based on cell rotation active light control technology

A technology of active light and light manipulation, applied in the field of biological optical tweezers and biological imaging, which can solve the problems of inconvenient operation and so on.

Pending Publication Date: 2021-10-19
GUILIN UNIV OF ELECTRONIC TECH
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0008] Dynamic speckle microscopic imaging technology has the ability of tomographic resolution. In order to obtain the three-dimensional structure of the object to be measured, it is necessary to obtain multi-layer tomographic images, which requires constant adjustment of the focus position of the microscope, which is inconvenient to operate.

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  • Speckle fluorescence microscopy method and system based on cell rotation active light control technology
  • Speckle fluorescence microscopy method and system based on cell rotation active light control technology
  • Speckle fluorescence microscopy method and system based on cell rotation active light control technology

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Embodiment Construction

[0023] The present invention will be further described in detail below in conjunction with examples, so that those skilled in the art can implement it with reference to the description.

[0024] It should be understood that terms such as "having", "comprising" and "including" used herein do not exclude the presence or addition of one or more other elements or combinations thereof.

[0025] A speckle fluorescence microscopy method and system based on cell rotation active light manipulation technology. It is characterized in that: the system is composed of two parts: a dynamic speckle illumination micro-imaging system and a light manipulation system. It consists of laser light sources 1, 13, 18; lenses 2, 3, 6, 7, 14, 15, 20, 21; micro displacement stage 4; scattering body 5; CMOS camera 8; imaging lens 9; optical filter 10; mirrors 11, 16; apochromatic microscope objectives 12, 17; cells to be measured 23; mirrors 22; acousto-optic deflector 19. In the system, the laser beam ...

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Abstract

The invention provides a speckle fluorescence microscopy method and a speckle fluorescence microscopy system based on a cell rotation active light control technology. The device is characterized in that a light control part of the device consists of three lasers. One laser beam completes stable capture of cells, and the other laser generates different deflection angles through an acousto-optic modulator and is focused by a microscope objective to alternately irradiate the two ends of the captured cells, so that accurate active light control of the rotation angle of the cells is realized. And after the cells rotate to different angles and reach a stable state, a third laser generates dynamic speckles to excite fluorophores in an illumination layer, a chromatographic image of the cells is obtained, and finally, a three-dimensional structure image of the cells is reconstructed. The system constructed by the invention can realize high-time and high-spatial-resolution three-dimensional structure imaging of living single cells, has the characteristics of simple structure, low cost, easiness in operation, non-invasion and the like, and has wide application prospects in many research fields such as biology, medicine, life science and the like.

Description

(1) Technical field [0001] The invention relates to a speckle fluorescence microscopy method and system based on cell rotation active light manipulation technology, which can be used for the capture and control of living single cells and the imaging of three-dimensional structures with high temporal resolution and high spatial resolution, belonging to biological Optical tweezers, bio-imaging fields. (2) Background technology [0002] Cell is the basic unit of the structure and function of organisms, and also the basic unit of life activities. The ability of cells to proliferate through division is the basis for the ontogeny and phylogeny of organisms. A cell is either an independent unit of life, or multiple cells form a cell population or tissue, or an organ, system or whole; a cell is the basic unit of heredity and has genetic totipotency. All living things, except viruses, are made up of cells. [0003] With the gradual and in-depth exploration of the nature of life ac...

Claims

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Application Information

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IPC IPC(8): G01N21/64G02B21/00G02B21/32G02B21/36
CPCG01N21/6458G01N21/6428G01N21/6402G01N21/645G02B21/0032G02B21/0076G02B21/32G02B21/361G01N2021/6439G01N2021/6471G01N2021/6478
Inventor 尹君王少飞于凌尧陈宏宇胡徐锦贾源苑立波
Owner GUILIN UNIV OF ELECTRONIC TECH