Soybean origin traceability identification method based on combination of MALDI-TOF/TOF and multi-element analysis technology

A technology of origin traceability and origin, which is applied in the direction of analyzing materials, material separation, instruments, etc., can solve the problems such as the inability to trace the origin of soybeans, and achieve the effect of improving the accuracy of traceability, improving accuracy, and improving accuracy

Pending Publication Date: 2021-10-19
TECH CENT OF GUANGZHOU CUSTOMS
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  • Claims
  • Application Information

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Problems solved by technology

This method cannot be used to establish a traceability model of soybean origin based on lipidomics

Method used

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  • Soybean origin traceability identification method based on combination of MALDI-TOF/TOF and multi-element analysis technology
  • Soybean origin traceability identification method based on combination of MALDI-TOF/TOF and multi-element analysis technology
  • Soybean origin traceability identification method based on combination of MALDI-TOF/TOF and multi-element analysis technology

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Experimental program
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Effect test

Embodiment 1

[0076] Soybean samples from different origins with definite regions were selected, and the soybean samples from each origin were physically pressed to obtain a standard soybean oil sample and ground to obtain a standard soybean flour sample.

[0077] The process of collecting mass spectrometry data of standard soybean oil samples is as follows: the standard soybean oil samples of different origins with definite regions are used as the matrix, and the standard soybean oil samples are printed on the target plate containing the matrix with a cotton swab Above, every six standard soybean oil samples were calibrated by PEG, and each standard soybean oil sample was measured three times in parallel. The target plate is placed in the matrix-assisted laser desorption ionization time-of-flight mass spectrometer. The working conditions of the matrix-assisted laser desorption ionization time-of-flight mass spectrometer are: the mass spectrometer adopts positive ion mode to collect data, th...

Embodiment 2

[0098]This embodiment only describes the difference from the above-mentioned embodiment. In the present embodiment, the mineral element content data collection method of the standard soybean meal sample is: the mineral element content data collection method of the standard soybean meal sample in step S2 is: Get 0.5g of standard soybean powder sample and place it in a polyethylene digestion tube, weigh 0.5g of standard soybean powder sample to an accuracy of 0.001g, add 4ml of nitric acid, cover the sealing cap, and soak for 6 to 12 hours; after soaking, put The sealed polyethylene digestion tube is placed in the digestion instrument, and inert gas is pre-filled in the inner tank of the digestion instrument. After cooling, take out the inner tank and catch the acid on the electric heating plate. The temperature set by the electric heating plate is 150°C. After acid removal, transfer the digestate in the inner tank to a 50mL polypropylene centrifuge tube, and wash the inner tank...

Embodiment 3

[0107] This embodiment only describes the differences from the above-mentioned embodiments. In this embodiment, the high-resolution mass spectrum data of triglyceride compounds in the standard soybean oil sample are sequentially normalized and averaged, and the partial least squares method -Discriminant analysis method, and the mineral elements in the soybean flour samples were processed by partial least squares method-discriminant analysis method, and the PLS-DA soybean origin traceability identification model based on MALDI-TOF / TOF and mineral element content analysis was constructed. Such as image 3 MALDI-TOF / TOF based models and such as Figure 9 The PLS-DA model based on mineral element content analysis shows that the PLS-DA origin traceability identification model can significantly distinguish soybean samples from Brazil, Argentina, Canada, and the United States. The characteristic contribution elements in American soybeans based on the mineral element content analysis ...

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Abstract

The invention discloses a soybean origin traceability identification method based on combination of MALDI-TOF / TOF and a multi-element analysis technology, and the soybean origin traceability identification method based on the fusion of MALDI-TOF / TOF and mineral element content analysis is established through the MALDI-TOF / TOF which is a novel soft ionization biomass spectrometry technology and in combination with a mineral element content analysis technology. By referring to the triglyceride compound high-resolution mass spectrum data of the soybean oil sample to be detected and the mineral element content in the soybean meal sample, the two kinds of data are fused and then imported into the soybean origin traceability identification model, the origin traceability prediction of the soybean to be detected is carried out, and the soybean origin traceability accuracy is improved.

Description

technical field [0001] The invention belongs to the technical field of origin traceability of crops, and in particular relates to a soybean origin traceability identification method based on the combination of MALDI-TOF / TOF and multi-element analysis technology. Background technique [0002] In recent years, with the trade war between China and the United States, China has begun to impose 25% tariffs on agricultural products such as soybeans from the United States, punishing and sanctioning agricultural products such as soybeans and soybean oil from the United States. During the trade dispute, some U.S. trade and export companies may transship U.S. soybeans and soybean oil to the Chinese market through Brazil, Argentina, Canada and other countries, pretending to be soybeans or soybean oil from these countries to avoid high tariffs in the United States. Statistics show that before the Sino-US trade dispute, China imported 96 million tons of soybeans in 2017, Brazil soybeans a...

Claims

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Application Information

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IPC IPC(8): G01N30/06G01N30/72
CPCG01N30/06G01N30/72
Inventor 王志元谢建军卢丽王璐李菊丁博侯颖烨陈文锐
Owner TECH CENT OF GUANGZHOU CUSTOMS
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