Preparation of double-index immunochromatography kit for detecting Adipo and SHBG

An immunochromatography test paper and immunochromatography technology, applied in the field of immunology, can solve the problem of not predicting the biological indicators of diabetes, etc., and achieve the effect of meeting the requirements of clinical testing and simple operation

Pending Publication Date: 2021-10-19
武汉市朗典精医生物科技有限公司
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Problems solved by technology

Traditional diabetes diagnosis uses fasting blood glucose, postprandial blood glucose and oral glucose tolerance test to detect glycosylated hemoglobin and glycosylated albumin indicators, but there are no biological indicators to predict the occurrence of diabetes, and biological indicators to predict the occurrence of diabetes need to be developed

Method used

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  • Preparation of double-index immunochromatography kit for detecting Adipo and SHBG
  • Preparation of double-index immunochromatography kit for detecting Adipo and SHBG
  • Preparation of double-index immunochromatography kit for detecting Adipo and SHBG

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Embodiment Construction

[0036] In conjunction with specific experiments, the principles and results of the present invention are further described, and the multi-step experimental process of the present invention is listed below.

[0037] 1. Preparation of Adipo / SHBG immunochromatographic test strips

[0038] (1) Adipo antibody coupled to europium chelate fluorescent microspheres

[0039] In the embodiment of the present invention, europium chelate (Europium Chelate) fluorescently labeled carboxyl-modified microparticles (Europium Chelate PS-COOH) with a diameter of 100-300 nm were selected from Bangs Laboratories Inc.

[0040] In the embodiment of the present invention, EDC and Sulfo-NHS were used to couple the Adipo monoclonal antibody purchased from MedixBiochemical to Europium Chelate PS-COOH, the steps are as follows:

[0041] 1. Take 0.1ml Europium Chelate PS-COOH with a concentration of 10mg / ml and a diameter of 200nm, add it to a 1.5ml centrifuge tube, centrifuge at 15000rpm for 15 minutes, ...

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Abstract

The invention belongs to the field of immunodetection, and particularly relates to a double-index immunochromatography kit for detecting adiponectin (Adipo) and sex hormone binding globulin (SHBG). The main component is an immunochromatography test strip, and the immunochromatography test strip is formed by sequentially pasting a sample pad, a binding pad, a nitrocellulose membrane and absorbent paper on a PVC base plate. Adipo antibody-microspheres and SHBG antibody-microspheres are adsorbed on the binding pad, an Adipo antibody is fixed on the nitrocellulose membrane to form a detection line 1 (T1 line), an SHBG antibody is fixed on the nitrocellulose membrane to form a detection line 2 (T2 line), and an anti-IgG antibody is fixed to form a quality control line (C line). The invention also discloses a process for coupling the Adipo antibody and the SHBG antibody with the microspheres. The Adipo/SHBG detection immunochromatography kit is used to determine the Adipo and SHBG concentrations in blood or other body fluids, and is used for early prediction of insulin resistance, type 2 diabetes mellitus and gestational diabetes mellitus occurrence risks.

Description

technical field [0001] The invention belongs to the field of immunology, and specifically relates to the process of preparing Adipo antibody-fluorescent microspheres and SHBG antibody-fluorescent microspheres and establishing a detection kit for Adipo / SHBG fluorescent immunochromatography. Background technique [0002] By 2030, the number of people with diabetes worldwide is expected to reach 360 million, and the vast majority of these cases are type 2 diabetes (Type 2 Diabetes Mellitus, T2DM). Diabetes is often associated with two metabolic defects, insulin resistance and impaired pancreatic β-cell function. However, factors such as genetic predisposition, obesity, pregnancy, and estrogenism contribute to the development of diabetes. Traditional diabetes diagnosis uses fasting blood glucose, postprandial blood glucose and oral glucose tolerance test to detect glycosylated hemoglobin and glycosylated albumin indicators, but there are no biological indicators to predict the ...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/558G01N33/543G01N33/532
CPCG01N33/558G01N33/54313G01N33/532G01N2800/042G01N2800/52
Inventor 乔洋洋
Owner 武汉市朗典精医生物科技有限公司
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