Instant collagen microneedle and preparation method thereof

A technology of collagen and recombinant collagen, which is applied in the field of skin care products, can solve the problems of poor solubility of animal collagen, poor formability of recombinant collagen, and difficulty in preparing soluble collagen microneedles, so as to achieve excellent mechanical properties of the needle body and excellent shape The effect of good sex and simple method

Pending Publication Date: 2021-10-22
胶原蛋白(武汉)生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] First of all, aiming at the defects of poor formability of recombinant collagen and poor solubility of animal collagen in the prior art, it is difficult to prepare soluble collagen microneedles, the present invention combines recombinant collagen / animal collagen with transparent Hyaluronic acid was mixed in a certain proportion to successfully prepare collagen microneedles with good formability and fast dissolution

Method used

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  • Instant collagen microneedle and preparation method thereof
  • Instant collagen microneedle and preparation method thereof
  • Instant collagen microneedle and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0052] Weigh hyaluronic acid, add quantitative water, and prepare hyaluronic acid solutions with concentrations of 1, 5, 10, 15, 20, 25, and 30 mg / ml; stir the solution until there are no powder agglomerates visible to the naked eye, and then put Put it into a vacuum drying oven and heat it to 80°C, and keep the temperature constant for 30 minutes; take it out and stir it evenly again, put it in a vacuum drying oven, keep a vacuum degree of -50kPa at 55°C, and take it out after drying and degassing for 12 hours; after cooling, add recombinant collagen to Its final concentration is: 0.5mg / ml, after stirring evenly, put it into a vacuum drying oven again, at a vacuum degree of -50kPa, dry and defoam at a constant temperature of 25°C for 12h to obtain a microneedle raw material solution.

[0053] Take a certain amount of microneedle raw material solution and add it to the PDMS mold, put it in a vacuum drying oven, dry at a constant temperature of -50kPa vacuum at 25°C for degassin...

Embodiment 2

[0059] Weigh hyaluronic acid, add quantitative water, and make a hyaluronic acid solution with a concentration of 15 mg / ml; stir the solution until there is no powder agglomeration visible to the naked eye, then put it into a vacuum drying oven and heat it to 65°C, and keep the temperature for 30min Stir evenly after taking it out, put it in a vacuum drying oven, keep the vacuum degree of -50kPa, 55°C, dry and defoam for 12 hours, then take it out; after cooling, add recombinant collagen to make the final concentration of recombinant collagen: 0.01, 0.1, 0.3, 0.5, 0.7, 1.0, 5.0, 10.0, 20.0, 30.0mg / ml, stirred evenly, put it into a vacuum drying oven again, with a vacuum degree of -50KPa, and dried at a constant temperature of 20°C for 12 hours to obtain a microneedle raw material solution.

[0060] Take a certain amount of microneedle raw material solution and add it to the PDMS mold, put it into a vacuum drying oven, and dry at a constant temperature of -50kPa vacuum at 20°C. ...

Embodiment 3

[0066] According to the experimental results of Example 1 and Example 2, in this example, the formation and dissolution of microneedles under the conditions of extreme hyaluronic acid concentration and extreme concentration of recombinant collagen were investigated. Weigh hyaluronic acid, add quantitative water, and make hyaluronic acid solutions with a concentration of 5mg / ml and 30mg / ml; stir the solution until there is no powder agglomeration visible to the naked eye, then put it in a vacuum drying oven and heat it to 65 ℃, constant temperature for 30min; after taking it out, stir again evenly, put it in a vacuum drying oven, keep the vacuum degree of -50kPa, 55℃, take it out after drying and defoaming for 12h; add recombinant collagen to the 30mg / ml hyaluronic acid group after cooling The concentration is 30mg / ml, add recombinant collagen to the 5mg / ml hyaluronic acid group until the concentration is 0.01mg / ml, stir evenly, then put it into the vacuum drying oven again, wit...

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Abstract

The invention belongs to the field of skin care products, and particularly relates to an instant collagen microneedle and a preparation method thereof. The collagen microneedle comprises recombinant collagen / animal collagen and hyaluronic acid, and the collagen microneedle prepared from a mixture solution of the recombinant collagen / animal collagen and hyaluronic acid is good in formability and solubility; the collagen microneedle also has a good microneedle shape, the mechanical property of the needle body is excellent, and the collagen microneedle can pierce the cuticle of the skin; the needle body can be quickly dissolved after being inserted into the skin, so that efficient delivery of collagen is realized, absorption of the collagen is promoted, and damaged skin is helped to be repaired; and the preparation method of the collagen microneedle is simple, the biological activity of the collagen is well maintained in the preparation process, and the preparation process is suitable for large-scale production.

Description

technical field [0001] The invention belongs to the field of skin care products, and in particular relates to an instant collagen microneedle and a preparation method thereof. Background technique [0002] Collagen is the most abundant protein in the human body, accounting for about 30% of the total protein in the human body. As the major structural protein, collagen forms a molecular scaffold that provides the body with mechanical strength and structural integrity, supporting and protecting soft tissues and internal organs. Collagen also has high biological activity, participates in the interaction of a large number of extracellular matrix, and has a very close relationship with cell regeneration and wound healing. Collagen has the advantages of biocompatibility, biodegradability and bioabsorption, so it is widely used in cosmetics, medical devices and other fields. [0003] Collagen is the main structural component of the skin. The degradation, breakage, collapse, and lo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K8/73A61K8/65A61K8/49A61K8/368A61K8/67A61K8/9789A61K8/9794A61K8/02A61Q19/00
CPCA61K8/735A61K8/65A61K8/4946A61K8/368A61K8/675A61K8/9789A61K8/9794A61K8/0204A61Q19/00A61K2800/91
Inventor 肖建喜李文华
Owner 胶原蛋白(武汉)生物科技有限公司
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